Icam1 fitc

Manufactured by R&D Systems

ICAM1-FITC is a fluorescently-labeled antibody that binds to the Intercellular Adhesion Molecule 1 (ICAM1) protein. ICAM1 is a cell surface protein involved in cell-cell adhesion and immune response. The FITC label allows for detection and visualization of ICAM1-expressing cells.

Automatically generated - may contain errors

Lab products found in correlation

2 protocols using icam1 fitc

Flow Cytometric Analysis of Cell Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

For flow cytometry analysis cells were washed with PBS and detached with TrypLE Express (Gibco). TrypLE neutralized with complete media and cells were centrifuged for 5 min at 300g. Cells were resuspended in blocking buffer (PBS without calcium and magnesium, 2.5 mM EDTA, 5% FBS) for 15min, centrifuged and subsequently stained for 45 min at 4C with antibodies CD31-BV421 (BD-biosciences), ICAM1-FITC (R&D) and respective isotype controls according to manufacturer’s indications. Samples were acquired with a BD LSR Fortessa 4L (BD-biosciences) flow cytometer and analyzed with FlowJo 10.8.0 software.

Carracedo M., Ericson E., Ågren R., Forslöw A., Madeyski-Bengtson K., Svensson A., Riddle R., Christoffersson J., González-King Garibotti H., Lazovic B., Hicks R., Buvall L., Fornoni A., Greasley P.J, & Lal M. (2023). APOL1 promotes endothelial cell activation beyond the glomerulus. iScience, 26(6), 106830.

+ Open protocol

+ Expand

Multiparametric Flow Cytometry for Cell Surface and Intracellular Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Anti-ICOSL (MIH12), ICOSL-APC, CD16-PE, anti–VCAM-1-PE, anti–E-Selectin-PE, CD27-PE, CD19-APC, CD45-PerCP/Cy5.5, goat anti-rabbit Alexa Fluor 488, goat anti-mouse Alexa Fluor 633, and Alexa Fluor 568 were from Thermo Fisher Scientific. CD19-FITC, IgD-FITC, FoxP3-A647, CD14-FITC, and CD45RA-PE were from BD Biosciences. Antibodies against RCAS1 and β-actin were from Cell Signaling Technology. HLA-DR-PerCP, CD11c-Pacific Blue, CXCR5-BV421, CCR7-APC/Cy7, PD1-PE/Cy7, and ICOS-APC were from BioLegend. BDCA1 (CD1c)-APC, BDCA3 (CD141)-PE, CD4-FITC, and ICAM-1-FITC were from R&D Systems.

Roussel L., Landekic M., Golizeh M., Gavino C., Zhong M.C., Chen J., Faubert D., Blanchet-Cohen A., Dansereau L., Parent M.A., Marin S., Luo J., Le C., Ford B.R., Langelier M., King I.L., Divangahi M., Foulkes W.D., Veillette A, & Vinh D.C. (2018). Loss of human ICOSL results in combined immunodeficiency. The Journal of Experimental Medicine, 215(12), 3151-3164.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!