Cell extracts were prepared as described previously89 (link). Western blotting was carried out using anti-NICD (4147, Cell Signaling) and anti-β-ACTIN antibodies (3G4-F9; AbFrontier), respectively.
Anti nicd 4147
Manufactured by Cell Signaling Technology
Anti-NICD (4147) is a primary antibody product offered by Cell Signaling Technology. It is designed to detect the Notch Intracellular Domain (NICD), a critical component involved in Notch signaling pathways.
Automatically generated - may contain errors
2 protocols using anti nicd 4147
1
Western Blot Analysis of NICD
2
Chromatin Immunoprecipitation and Real-time PCR
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ChIP was performed as described previously89 (link). Anti-JMJD3 antibody (AP1022a, Abgent), anti-NICD (4147, Cell Signaling), anti-trimethyl H3K27 antibody (07–449, Millipore), anti-monomethyl H3K27 antibody (07–448, Millipore), anti-phosphorylated RNA polymerase II antibody (ab5131, Abcam), anti-p50 antibody (ab7971, Abcam), anti-p65 antibody (ab7970, Abcam), or normal IgG (SC-2027, Santa Cruz Biotechnology) were used. Real-time PCR was performed with a Stratagene Mx3000P using primers (see Supplementary Table S2 ).
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