Oleic acid and 1-octadecene (90%) were purchased from Sigma-Aldrich. NHS–PEG2000–cholestrol were purchased from Ponsure Biological (Shanghai, China). NaOH, OA and NH4F were obtained from Damas-beta (Shanghai, China). Cyclohexane and chloroform (CHCl3) were purchased from General-reagent company. All reagents were of analytical grade and used without further purification. RPMI 1640 medium, penicillin–streptomycin solution, fetal bovine serum (FBS) and phosphate buffer saline (PBS) were acquired from Gibco Company (USA). The cell cultivation mixture consisted of RPMI 1640 medium, FBS (10%), and penicillin–streptomycin solution (1%). Sulfo-Cyanine5.5 NH2 was obtained from Shanghai Maokang Biotechnology Co., Ltd. Bevacizumab was purchased from MedChemExpress (Shanghai, China). Cell Counting Kit 8 assay was purchased from 7Sea Biotech Company (Shanghai, China). Deionized water (DI water, 18.2 MΩ) was acquired from a Milli-Q Millipore Water System (Millipore, MA, USA) and used in all experiments. An AniView 100 living body imaging system (BLT, China) was used to observe Cy5.5 fluorescence from NPBCNs, using the Cy5.5 filter sets (excitation: 673 nm and emission: 707 nm). The near-infrared fluorescent dye Cy5.5 has an emission wavelength beyond the visible range, and the fluorescence pseudocolor assigned to Cy5.5 was red.
Cell counting kit 8 assay
Manufactured by 7Sea Biotech
Sourced in China
The Cell Counting Kit-8 (CCK-8) assay is a colorimetric method for determining the number of viable cells in a cell proliferation or cytotoxicity assay. The assay uses a water-soluble tetrazolium salt to measure the metabolic activity of cells, which is proportional to the number of living cells.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin streptomycin solution, by Thermo Fisher Scientific (1 mentions)
Bevacizumab, by MedChemExpress (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Oleic acid, by Merck Group (1 mentions)
1 octadecene, by Merck Group (1 mentions)
Prism 8, by GraphPad (1 mentions)
6 protocols using cell counting kit 8 assay
1
Nano-Bioconjugate Imaging Protocol
2
Epirubicin Cytotoxicity Evaluation
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The viability of the cells treated with various concentrations of epirubicin (0.5, 1.0, 1.5, and 2.0 μM) was determined by the Cell Counting Kit-8 assay (CCK-8, 7Sea Biotech, Shanghai, China) as previously described [14 (link)]. Cells (5,000/well) were seeded into 96-well plates for 24 h and treated with epirubicin for 48 h. The half-maximum inhibitory concentration (IC50) was calculated by nonlinear regression analysis using GraphPad Prism 8.0 software (GraphPad Software, La Jolla, CA, USA).
3
Cell Viability Assay Protocol
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Cell viability was measured using the Cell Counting Kit-8 assay (CCK-8; 7Sea Biotech, Shanghai, China). Transfected cells (A549 and H1299) were seeded into 96-well plates at a density of 2000 cells/well (n = 5 for each time point) in a final volume of 100 μl. CCK-8 solution (10 μl) was added to each well at 24-, 48-, 72-, 96-, and 120-h time points. The absorbance at 450 nm was measured after incubation for 2 h at 37 °C to calculate the number of viable cells.
4
Measuring Cell Viability with CCK-8 Assay
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Cell viability was measured using the Cell Counting Kit-8 assay (CCK-8; 7Sea Biotech, Shanghai, China). Transfected cells (A549 and H1299) were seeded into 96-well plates at a density of 2000 cells/well (n = 5 for each time point) in a final volume of 100 μl. CCK-8 solution (10 μl) was added to each well at 24-, 48-, 72-, 96-, and 120-h time points. The absorbance at 450 nm was measured after incubation for 2 hours at 37°C to calculate the number of viable cells.
5
Cell Viability Assay Protocol
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Cell viability was measured using the Cell Counting Kit-8 assay (CCK-8; 7Sea Biotech, Shanghai, China).
Transfected cells (A549 and H1299) were seeded into 96-well plates at a density of 2000 cells/well (n = 5 for each time point) in a nal volume of 100 μl. CCK-8 solution (10 μl) was added to each well at 24-, 48-, 72-, 96-, and 120-h time points. The absorbance at 450 nm was measured after incubation for 2 hours at 37°C to calculate the number of viable cells.
Transfected cells (A549 and H1299) were seeded into 96-well plates at a density of 2000 cells/well (n = 5 for each time point) in a nal volume of 100 μl. CCK-8 solution (10 μl) was added to each well at 24-, 48-, 72-, 96-, and 120-h time points. The absorbance at 450 nm was measured after incubation for 2 hours at 37°C to calculate the number of viable cells.
6
Cell Viability Assay Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell viability was measured using the Cell Counting Kit-8 assay (CCK-8; 7Sea Biotech, Shanghai, China).
Transfected cells (A549 and H1299) were seeded into 96-well plates at a density of 2000 cells/well (n = 5 for each time point) in a nal volume of 100 μl. CCK-8 solution (10 μl) was added to each well at 24-, 48-, 72-, 96-, and 120-h time points. The absorbance at 450 nm was measured after incubation for 2 hours at 37°C to calculate the number of viable cells.
Transfected cells (A549 and H1299) were seeded into 96-well plates at a density of 2000 cells/well (n = 5 for each time point) in a nal volume of 100 μl. CCK-8 solution (10 μl) was added to each well at 24-, 48-, 72-, 96-, and 120-h time points. The absorbance at 450 nm was measured after incubation for 2 hours at 37°C to calculate the number of viable cells.
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