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2 protocols using trim33

1

Antibody Characterization and Applications

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The following antibodies were from Sigma: anti-hemagglutinin (HA)-tag (H3663; 1:1,000 IB), anti- FLAG-tag (F1804; 1:1,000 IB, 2 μg IP), and anti-Myc-tag (M4439; 1:1,000 IB, 2 μg IP). The following antibodies were from Cell Signaling Technology: non-phosphor (active) β-catenin (#8814; 1:100 IHC), Axin2 (#2151; 1:1,000 IB), β-TrCP (#4394; 1:1,000 IB), PKCδ (#2058; 1:1,000 IB), and phosphor-PKCδ (Ser643/676) (#9376; 1:1,000 IB). The following antibodies were from Santa Cruz Biotechnology: β-catenin (sc-7963; 1:1,000 IB, 2 μg IP), PKCδ (sc-937; 1:100 IF), Lamin B (sc-6216; 1:1,000 IB), tubulin (sc-8035; 1:1,000 IB), normal rabbit IgG (sc-2345; 2 μg IP), normal mouse IgG (sc-2025; 2 μg IP), β-actin (sc-47778; 1:10,000 IB), GST (sc-138; 1:1,000 IB), GSK-3β (sc-9166; 1:1,000 IB) and Cyclin D1 (sc-753; 1:1,000 IB). The following antibodies were from Bethyl Laboratories: TRIM33 (A301-060A; 1:1,000 IB, 2 μg IP), TRIM33 (IHC-00216; 1:100 IHC). The following antibody was from BD Transduction Laboratories: β-catenin (610153; 1:100 IF). The following antibody was from Abcam: Na-K-ATPase (ab76020; 1:1,000 IB). The phosphor-Ser715 β-catenin antibody (1:1,000 IB; 1:100 IHC) was produced by Signalway Antibody (College Park, MD).
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2

Antibody Characterization and Applications

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were from Sigma: anti-hemagglutinin (HA)-tag (H3663; 1:1,000 IB), anti- FLAG-tag (F1804; 1:1,000 IB, 2 μg IP), and anti-Myc-tag (M4439; 1:1,000 IB, 2 μg IP). The following antibodies were from Cell Signaling Technology: non-phosphor (active) β-catenin (#8814; 1:100 IHC), Axin2 (#2151; 1:1,000 IB), β-TrCP (#4394; 1:1,000 IB), PKCδ (#2058; 1:1,000 IB), and phosphor-PKCδ (Ser643/676) (#9376; 1:1,000 IB). The following antibodies were from Santa Cruz Biotechnology: β-catenin (sc-7963; 1:1,000 IB, 2 μg IP), PKCδ (sc-937; 1:100 IF), Lamin B (sc-6216; 1:1,000 IB), tubulin (sc-8035; 1:1,000 IB), normal rabbit IgG (sc-2345; 2 μg IP), normal mouse IgG (sc-2025; 2 μg IP), β-actin (sc-47778; 1:10,000 IB), GST (sc-138; 1:1,000 IB), GSK-3β (sc-9166; 1:1,000 IB) and Cyclin D1 (sc-753; 1:1,000 IB). The following antibodies were from Bethyl Laboratories: TRIM33 (A301-060A; 1:1,000 IB, 2 μg IP), TRIM33 (IHC-00216; 1:100 IHC). The following antibody was from BD Transduction Laboratories: β-catenin (610153; 1:100 IF). The following antibody was from Abcam: Na-K-ATPase (ab76020; 1:1,000 IB). The phosphor-Ser715 β-catenin antibody (1:1,000 IB; 1:100 IHC) was produced by Signalway Antibody (College Park, MD).
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