Ixon 3 860

Manufactured by Oxford Instruments

Sourced in Canada

The IXon 3 860 is a scientific-grade back-illuminated EMCCD camera designed for low-light imaging applications. It features a high quantum efficiency sensor and advanced EMCCD technology to enable single-photon detection. The core function of the IXon 3 860 is to provide high-sensitivity, high-resolution imaging for various scientific research and industrial applications.

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Lab products found in correlation

Ixon ultra, by Oxford Instruments (2 mentions) Uplansapo 100 xo, by Olympus (2 mentions) Fpl 1055 t, by Thorlabs (2 mentions) Inug2 onics, by Tokai Hit (2 mentions) Rhod 2, by Thermo Fisher Scientific (1 mentions)

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IXon 860 (2 citations)

4 protocols using ixon 3 860

High-speed Cardiac Myocyte Imaging

Cited 1 time

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The optical system used was similar to that used in our previous work, with some modifications^{17 (link),18 (link)}. Briefly, an inverted microscope (IX-70, Olympus) was equipped with a high-sensitivity camera (iXon 3 860, iXon Ultra, Andor Technology) and an oil immersion objective (UPlanSapo 100 XO, Olympus). The vibration noise of the microscope was reduced by making the microscope stage rigid and optimizing the performance of a vibration-free table. The size of a pixel on the camera corresponded to 150 nm in the sample. The frame rate was adjusted to 500 fps for high-temporal-resolution analysis. The temperatures of the glass slides seeded with cardiomyocytes were controlled to 37.0 ± 0.2 °C by a thermostatically controlled incubator on the sample stage (INUG 2—ONICS, Tokai Hit Co.). A 1550 nm laser (FPL 1055 T, Thorlabs) was used as a heat source to enable rapid alteration of temperature in the vicinity of the cardiac myocytes being monitored. A 488 nm laser (FITEL HPU50211 (Blue), Furukawa Electric) was used as an excitation light source for fluorescence observation of AcGFP-actinin.

Shintani S.A., Washio T, & Higuchi H. (2020). Mechanism of contraction rhythm homeostasis for hyperthermal sarcomeric oscillations of neonatal cardiomyocytes. Scientific Reports, 10, 20468.

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Characterizing Calcium Dynamics in Cardiac Cells

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NRVMs in 3-D constructs and 2-D monolayers were stained with 5μM Ca²⁺-sensitive dye Rhod-2 (Invitrogen, Carlsbad, CA), excited by green light (520±33 nm), and imaged using CMOS (Ultima-L, SciMedia) or EMCCD (iXon3 860, Andor) cameras and custom-designed optics. Bipolar point or line electrodes were used to locally stimulate NRVMs and initiate action potential propagation. Conduction velocities were estimated from mapped Ca²⁺ waves with activation times assigned as times of maximum upstroke of Ca²⁺ transients. The shape of Ca²⁺ transient was characterized by applying field shock to simultaneously excite the cells in the patch followed by measurement of time-to-peak (TPT, from activation time to peak of the transient), 50% relaxation time (RT₅₀, the time from the peak to 50% recovery), and 80% relaxation time (RT₈₀, the time from the peak to 80% recovery) [26 (link)].

Bian W., Badie N., Himel HD I.V, & Bursac N. (2014). Robust T-Tubulation and Maturation of Cardiomyocytes Using Tissue-Engineered Epicardial Mimetics. Biomaterials, 35(12), 3819-3828.

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Cardiomyocyte Heat-Induced Spontaneous Oscillations

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We used previous data [8 (link)] acquired as briefly described below. An inverted microscope (IX-70, Olympus) was equipped with a high-sensitivity camera (iXon 3 860, iXon Ultra, Andor Technology) and an oil-immersed objective lens (UPlanSapo 100 XO, Olympus). The pixel size of the camera corresponded to the cell length of 150 nm, and a frame rate of 500 fps was used for high-resolution temporal analysis. The temperature of the glass slide seeded with cardiomyocytes was maintained at (37.0±0.2)°C using a thermostat-controlled incubator (INUG 2-ONICS, Tokai Hit) on the sample stage. A 1550 nm laser (FPL 1055 T, Thorlabs) was used as a heat source to rapidly change the temperature near the cardiomyocytes to be monitored. HSOs were induced by application of heat in the vicinity of cardiomyocytes. A 488 nm laser (FITEL HPU50211, Furukawa Electric Co., Ltd.) was used as an excitation light source for observing the fluorescence of AcGFP-α-actinin.
The observation of HSOs with simultaneous measurement of changes in Fluo4 fluorescence intensity used previous data [7 (link)]. In this observation, HSOs were observed in cases where there was a change in intracellular calcium concentration (HSOs with beating) and when the intracellular calcium concentration was maintained constant in the same manner as during Cell-SPOCs observation (HSOs without beating).

, & Shintani S.A. (2024). Observation of sarcomere chaos induced by changes in calcium concentration in cardiomyocytes. Biophysics and Physicobiology, 21(1), e210006.

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Two-Color Single-Molecule ALEX Microscopy

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The optical part of the custom-built setup was an inverted fluorescence microscope with wide-field illumination and two-colour alternating laser excitation (ALEX). For a detailed schematic and for filter information, refer to Supplementary Section 4. The excitation source consists of two diode-pumped solid-state (DPSS) lasers. The green laser is centred at 532 nm (Laser Quantum gem, power range: 50 – 500 mW). The red laser is centred at 638 nm (Coherent CUBE, 25 mW, maximum power 50 mW).
Images were recorded using an electron multiplying charge-coupled device (EMCCD) camera (Andor iXon3 860), operated with an EM gain of 250, temperature −60 °C and frame rate 20 fps. The EMCCD chip hosts a two dimensional array of 128 × 128 light-sensitive pixels. The combination of objective and tube lens results in a lateral magnification of ~100×, corresponding to ~0.24 μm per pixel.

Hemmig E.A., Fitzgerald C., Maffeo C., Hecker L., Ochmann S.E., Aksimentiev A., Tinnefeld P, & Keyser U.F. (2018). Optical Voltage Sensing Using DNA Origami. Nano Letters, 18(3), 1962-1971.

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