The tissues were fixed with 10% formaldehyde and embedded in paraffin followed by incubation of target primary antibody for overnight and goat anti-rabbit IgG-biotin secondary antibody (1:300, Abcam) for 1 h. After extensive washings with PBS, the samples were stained with diaminobenzidine peroxidase substrate and photographed.
Goat anti rabbit igg biotin secondary antibody
Manufactured by Abcam
Sourced in United States
Goat anti-rabbit IgG-biotin secondary antibody is a reagent used in immunoassays and other immunological techniques. It is designed to detect and bind to rabbit primary antibodies, allowing for further detection or signal amplification.
Automatically generated - may contain errors
Lab products found in correlation
Aperio software, by Leica (1 mentions)
Anti hif 1α, by Santa Cruz Biotechnology (1 mentions)
Anti vegf, by Santa Cruz Biotechnology (1 mentions)
Anti phospho mtor, by Cell Signaling Technology (1 mentions)
Anti akt, by Cell Signaling Technology (1 mentions)
Anti β actin, by Santa Cruz Biotechnology (1 mentions)
Anti α tubulin, by Santa Cruz Biotechnology (1 mentions)
Anti vegfr2, by Cell Signaling Technology (1 mentions)
Anti phospho vegfr2, by Cell Signaling Technology (1 mentions)
Anti mtor, by Cell Signaling Technology (1 mentions)
Anti phospho akt, by Cell Signaling Technology (1 mentions)
Z ligustilide, by Merck Group (1 mentions)
Horseradish peroxidase hrp labeled secondary antibody, by Abcam (1 mentions)
3 protocols using goat anti rabbit igg biotin secondary antibody
1
Immunohistochemistry Protocol for Paraffin-Embedded Tissues
2
Immunohistochemical Analysis of Tumor Tissues
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Tumor tissues were fixed with 10% formaldehyde and embedded in paraffin followed by incubation of target primary antibody for overnight and goat anti-rabbit IgG-biotin secondary antibody (1:300, Abcam, MA, USA) for 1 h. After extensive washings with PBS, the samples were stained with diaminobenzidine peroxidase substrate and photographed and quantitated using Aperio® software (Leica Biosystems, Vista, CA, USA).
3
Antibody Reagents for Cell Signaling
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The antibodies including anti-HIF-1α, anti-VEGF, anti-CD31 (PECAM-1), anti-VHL, anti-WSB-1, anti-β-actin, anti-α-tubulin and fluorescein isothiocyanate (FITC)-coupled secondary antibody were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The anti-VEGFR2, anti-phospho-VEGFR2, anti-AKT, anti-phospho-AKT, anti-mTOR, and anti-phospho-mTOR were purchased from Cell Signaling Technology (Danvers, MA, USA). Horseradish peroxidase (HRP)-labeled secondary antibody and goat anti-rabbit IgG-biotin secondary antibody were obtained from Abcam (Cambridge, MA, USA). The z-ligustilide, n-butylidenephthalide, and other chemical reagents used in this study were purchased from Sigma (St. Louis, MO, USA)
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