B6.129p2 leprtm1rck j

To generate ALKO mice, we used a cre recombinase transgenic mouse under the control of a mouse glial fibrillary acidic protein (GFAP) promoter. The GFAP-cre mice were B6.Cg-Tg(Gfap-cre)73.12Mvs/J (Stock number 012886, Jackson Laboratory, Bar Harbor, MN) that contain a mouse GFAP promoter sequence directing expression of Cre recombinase (Garcia et al., 2004 (link)). The LepR-floxed mice were B6.129P2-Lepr^tm1Rck/J (Stock number 008327, Jackson Laboratory), with loxP sites on either side of exon 1 (Cohen et al., 2001 (link)). GFAP^cre/+ hemizygous transgenic mice and LepR^loxP/loxP homozygote mice were bred for two generations. The hemizygotes of the F1 generation were backcrossed with LepR-floxed mice, to select the F2 generation of ALKO-Δ1 mice that express both GFAP-cre and LepR-floxed alleles on tail genotyping. The agarose gel electrophoresis of PCR products and genotyping primers are shown in figure 1A and table 1.