Hrp conjugated goat anti mouse

Manufactured by Thermo Fisher Scientific

Sourced in United States

HRP-conjugated goat anti-mouse is a secondary antibody that binds to mouse primary antibodies. It is conjugated with horseradish peroxidase (HRP), which enables detection and visualization of target proteins in various immunoassays and immunohistochemical applications.

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Lab products found in correlation

Hrp conjugated goat anti rabbit, by Thermo Fisher Scientific (3 mentions) Monomeric avidin agarose, by Thermo Fisher Scientific (2 mentions) Image lab 6, by Bio-Rad (2 mentions) Chemidoc xrs system, by Bio-Rad (2 mentions) Clarity western ecl substrate, by Bio-Rad (2 mentions) Ecl plus substrate, by PerkinElmer (1 mentions) Tgx tris glycine gels, by Bio-Rad (1 mentions) Microbca kit, by Thermo Fisher Scientific (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Pierce ecl western blotting substrate kit, by Thermo Fisher Scientific (1 mentions) Colchicine, by Merck Group (1 mentions) Propidium iodide, by Thermo Fisher Scientific (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Tamoxifen, by Merck Group (1 mentions) 4 hydroxytamoxifen, by Merck Group (1 mentions) 5 5 dithiobis 2 nitrobenzoic acid, by Merck Group (1 mentions) Incucyte, by Sartorius (1 mentions) Valspodar psc 833, by Merck Group (1 mentions) Tween 20 p9416, by Merck Group (1 mentions) 2 7 dichlorofluorescin diacetate, by Merck Group (1 mentions)

Spelling variants of this product

Goat anti-mouse HRP (78 citations) HRP-conjugated goat anti-mouse IgG (H + L) secondary antibody (9 citations) HRP-conjugated goat-anti mouse or rabbit IgG (7 citations) HRP-anti-mouse (6 citations) HRP)-conjugated Goat anti-mouse IgG1 (5 citations) HRP-conjugated goat anti-mouse or goat anti-rabbit secondary antibodies (4 citations) Poly horseradish peroxidase (poly HRP)-conjugated goat anti-mouse antibody (2 citations) HRP-conjugated goat anti-mouse and anti-rabbit IgG (2 citations) HRP-conjugated goat anti-mouse IgG2c (2 citations) Goat anti-mouse IgG (H+L) secondary antibody conjugated to HRP (2 citations)

18 protocols using hrp conjugated goat anti mouse

Alcian Blue Staining and Immunoblotting

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Samples were separated on 4–20% BioRad TGX Tris-glycine gels and stained overnight with alcian blue as in [40 (link)]. Gels were imaged with a G-box QX4 with white-light converter (Syngene). Band intensity was quantified with GeneQuant (Syngene) via the manual band quantification method, using inter-lane spaces as background. For detection of phosphotyrosines, lysates were blotted onto PVDF membranes, incubated with 4G10 monoclonal antibodies (Millipore; 1:1000 dilution) followed by HRP-conjugated goat anti-mouse (Invitrogen), and developed with ECL-plus substrate (Perkin Elmer). Blots were stripped and re-probed with antiserum raised against Bacillus subtilis isocitrate dehydrogenase (ICDH).

Geisinger E, & Isberg R.R. (2015). Antibiotic Modulation of Capsular Exopolysaccharide and Virulence in Acinetobacter baumannii. PLoS Pathogens, 11(2), e1004691.

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Investigating Cellular Stress Responses

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Tamoxifen, 4-hydroxy-Tamoxifen, Dimethylsulfoxide, 2’,7’-dichlorofluorescin diacetate, 5,5’-dithio-bis (2-nitrobenzoic acid), colchicine, valspodar (PSC-833) and Tween-20 (P9416) were all purchased from Sigma Aldrich. Lipofectamine 2000, the microBCA kit and the Pierce™ ECL Western Blotting Substrate Kit were all products of Thermo Fisher. Propidium iodide and the HRP-conjugated goat anti-mouse were purchased from Invitrogen, and the IncuCyte® is a product of Essen BioScience.

Bakadlag R., Limniatis G., Georges G, & Georges E. (2023). The anti-estrogen receptor drug, tamoxifen, is selectively Lethal to P-glycoprotein-expressing Multidrug resistant tumor cells. BMC Cancer, 23, 24.

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Antibody Validation for Nephrin Signaling

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Primary antibodies used for these experiments were as follows: mouse anti-Myc (Cell Signalling, 9B11), mouse anti-phosphotyrosine 4G10 (#16–101; Upstate Biotechnology), rabbit anti-podocin (Sigma Aldrich, P0372) and mouse anti-β-actin (Sigma Aldrich, A1978). Rabbit anti-nephrin antibodies were provided by Dr. Tomoko Takano (McGill University) [10 (link)]. Phospho-specific anti-nephrin antibodies (Y1176/Y1193 and Y1217) were generated and validated previously [15 (link)]. Secondary antibodies (all Invitrogen) included horseradish peroxidase (HRP)-conjugated goat anti-rabbit (#A11008) and HRP-conjugated goat anti-mouse (#A11001), as well as goat anti-mouse Alexa Fluor-conjugated 594 (#A11005).

Cooper C.J., Dutta N.T., Martin C.E., Piscione T.D., Thorner P.S, & Jones N. (2018). Characterization of a novel disease-associated mutation within NPHS1 and its effects on nephrin phosphorylation and signaling. PLoS ONE, 13(9), e0203905.

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Optimizing Antibody Selection for Western Blotting and Immunofluorescence

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Culture media, FBS, dimethyloxalylglycine (DMOG), and antibodies against FLAG-tag (F7425, 1:5000 dilution), and HA-tag (11867423, 1:10,000) were purchased from Sigma-Aldrich (St. Louis, MO). Antibodies against FIH (sc-271780, 1:5000 for Western blotting; sc-21219, 1:200 for Immunofluorescence), GST (sc-138, 1:10,000), β-tubulin (sc-9104, 1:5000), Lamin-B (sc-6216, 1:1000), VHL (sc-55506, 1:500) and HRP-conjugated goat anti-rat secondary antibody (sc-2006, 1:5000) were from Santa Cruz Biotechnology (Santa Cruz, CA). HRP-conjugated goat anti-rabbit (G21234, 1:5000), HRP-conjugated goat anti-mouse (G21040, 1:5000), Alexa Fluor 488 rabbit anti-goat (A11078, 1:1000) and Alexa Fluor 594 goat anti-rat (A11007, 1:1000) secondary antibodies were purchased from Invitrogen (Carlsbad, CA); anti-acetylated lysine antibody (9441, 1:4000) from Cell Signaling Technology (Danvers, MA); anti-His(6)-tag antibody (PM032, 1:1000) from MBL (Nagoya, Japan). Anti-HIF-1α (1:1000) and anti-NAA10 antibodies (1:1000 for Western blotting; 1:200 for Immunofluorescence) were generated in rabbits, as previously described [8] (link), [20] (link). The antibody against N803-hydroxylated HIF-1α (1:1000) was a generous gift from Dr. Myung-Kyu Lee (Korea Research Institute of Bioscience & Biotechnology, Korea) [21] (link).

Kang J., Chun Y.S., Huh J, & Park J.W. (2018). FIH permits NAA10 to catalyze the oxygen-dependent lysyl-acetylation of HIF-1α. Redox Biology, 19, 364-374.

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Antibody Binding Assay for CHO Cells

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CHO-PNAd (2×10⁴) cells were coated onto 96-well plates and incubated overnight at 37°C. After five washes with PBS buffer, the plates were fixed with 4% paraformaldehyde and blocked with 3% BSA. The antibody dilutions starting at 1ng/ml to 300μg/ml were then added to the wells. After five washes in PBST, secondary antibodies, HRP–conjugated goat anti-mouse (31430, Invitrogen) and anti-rat (31470, Invitrogen) were added at a 1:10,000 dilution and incubated for 1 h at room temperature. Binding was detected with the addition of TMB substrate (N301, Thermo Scientific), and the reaction was stopped by adding TMB Stop Solution (N600, Thermo Scientific). Absorbance signals were read at 450 nm. Isotyping ELISA was performed using Rapid ELISA Mouse mAb Isotyping Kit (37503, Thermo Scientific).

Jiang L., Jung S., Zhao J., Kasinath V., Ichimura T., Joseph J., Fiorina P., Liss A.S., Shah K., Annabi N., Joshi N., Akama T.O., Bromberg J.S., Kobayashi M., Uchimura K, & Abdi R. (2020). Simultaneous targeting of primary tumor, draining lymph node, and distant metastases through high endothelial venule-targeted delivery. Nano today, 36, 101045.

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Western Blot Analysis of Fascin-1 Expression

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Cells were harvested 72 h post transfection and lysed in RIPA buffer. Cell lysates were separated by electrophoresis on 12% SDS–polyacrylamide gels, transferred to PVDF membranes (Millipore, Billerica, MA, USA), and probed with primary antibodies: mouse to Fascin-1 (1 : 1000) (Abcam, Cambridge, MA, USA) and mouse to anti-β-actin (1 : 2000) (Cell Signaling Technology, Danvers, MA, USA). After washing, the membranes were incubated with secondary antibody HRP-conjugated goat anti-mouse (1 : 5000 dilution; Invitrogen) and visualised by enhanced chemiluminescence.

Feng Y., Zhu J., Ou C., Deng Z., Chen M., Huang W, & Li L. (2014). MicroRNA-145 inhibits tumour growth and metastasis in colorectal cancer by targeting fascin-1. British Journal of Cancer, 110(9), 2300-2309.

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Quantifying Protein Expression via Western Blot

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Protein extraction and Western blotting were carried out as described previously, with some modifications (38 (link)). Total protein was extracted from a confluent 15 cm dish of cells and suspended in 50 mM Tris, 10 mM EGTA, with protease inhibitors (Roche). Lysates were diluted in Laemmli buffer at a ratio of 4:1 and heated at 80 °C for 10 min. Protein (30–100 μg) was separated by SDS-PAGE (12% acrylamide, 120 V, 2 h) and transferred onto a nitrocellulose (1.3 A, 25 V, 10 min) or a PVDF (2.5 A, 25 V, 3 min) membrane by semi-dry transfer. Primary antibodies were rabbit anti-GFP (ab6556, 1:2500, Abcam) or mouse anti-α-tubulin (clone DM1A, 1:10,000, Sigma). Secondary antibodies were HRP-conjugated goat anti-mouse (1:1000, Thermo Scientific) or goat anti-rabbit (1:1000, Thermo Scientific). Chemiluminescence was detected using an iBRIGHT imaging system (Invitrogen) or X-ray film (Fujifilm) following West Dura application (5 min, Thermo Scientific). Densitometry was performed on Western blot bands to estimate protein quantity using ImageJ 1.51i (81 ).

Haworth A.S., Hodges S.L., Capatina A.L., Isom L.L., Baumann C.G, & Brackenbury W.J. (2022). Subcellular dynamics and functional activity of the cleaved intracellular domain of the Na+ channel β1 subunit. The Journal of Biological Chemistry, 298(8), 102174.

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CocH5-Fc(M6) Fc Fusion Protein Characterization

Cited 2 times

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CocH5-Fc(M6) is an Fc fusion protein with CocH5 (which is the A199S/F227A/P285A/S287G/A328W/Y332G mutant of human butyrylcholinesterase) fused with a mutant (A1V/M38Y/S40T/T42E/D142E/L144M) of Fc region of human IgG1, and it was developed in our previous studies.^{18 (link), 34 (link), 36 (link)} Cocaine was provided by the National Institute on Drug Abuse (NIDA) Drug Supply Program (Bethesda, MD). Anti-DAT antibody (mouse monoclonal antibody, catalog #MA524796), and HRP-conjugated goat anti-mouse (catalog #G21040) polyclonal antibody, protease and phosphatase inhibitor cocktail (100-X), Sulfo-NHS-SS-biotin (sulfosuccinimidyl-2-[biotinamido]ethyl-1,3-dithiopropionate), and monomeric avidin agarose were purchased from Thermo Fisher Scientific (Waltham, MA). Antibodies for rat Na+/K+ ATPase (mouse monoclonal antibody, catalog #sc-48345), β-actin (Horseradish peroxidase (HRP)-conjugated mouse polyclonal antibody, catalog #sc-47778), and protein phosphatase 2A (PP2A, mouse monoclonal antibody, catalog #sc-13601) were all ordered from Santa Cruz Biotechnology (Santa Cruz, CA). All other chemicals and surgery tools were purchased from either VWR international (Radnor, PA), Thermo Fisher Scientific (Waltham, MA), or Sigma-Aldrich (St. Louis, MO).

Deng J., Zheng X., Shang L., Zhan C.G, & Zheng F. (2022). Gender differences in cocaine-induced hyperactivity and dopamine transporter trafficking to the plasma membrane. Addiction biology, 27(6), e13236.

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Profiling Antigen Presentation Machinery

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The protein concentration of each fraction was measured by a Bradford protein assay. An amount of 5 µg protein of each fraction was separated on 10% polyacrylamide gels at 40 mA per gel for 2 h and were blotted onto nitrocellulose membranes by semi-dry Western blot at 180 mA per membrane for 1 h. Membranes were blocked in 5% skim milk powder in TBS-T, incubated with the indicated primary antibody solution (in TBS-T supplemented with 5% BSA and 0.1% sodium azide) at 4 °C overnight followed by incubation with corresponding HRP-conjugated secondary antibody solution (in 3% BSA in TBS-T) at RT for 1 h. Proteins were visualized with ECL Detection Reagents (GE Healthcare, Chicago, IL, USA). The antibodies used were anti-beta Actin (MP, Irvine, CA, USA), anti-Tapasin, anti-beta2-Microglobulin, anti-ERp57, anti-Calreticulin (all Abcam, Cambridge, UK), anti-Yes (BD Bioscience), anti-Flotillin1, anti-Caveolin1 (Sigma-Aldrich, Seelze, Germany), anti-TAP1 (Novus Biologicals, Cambridge, UK), anti-TAP2 (MBL, Woburn, MA, USA), HRP-conjugated goat anti mouse and HRP-conjugated goat anti-rabbit (Thermo Scientific, Bonn, Germany).

Hoppmann N., Heinig N., Distler U., Kim E., Lennerz V., Krauß Y., Schumann U., Giese A., Tenzer S., Bitar L, & Schmidt M.H. (2022). Gamma Irradiation Triggers Immune Escape in Glioma-Propagating Cells. Cancers, 14(11), 2728.

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Reagents for P2X7 Receptor Signaling

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Carbenoxolone (CBX), BAPTA-AM and thapsigargin (TG) were purchased from Tocris Bioscience (Ellisville, MO). APT102, the recombinant form of soluble CD39, was kindly provided by Dr. Ridong Chen at APT Therapeutics Inc. (St. Louis, MO). All other chemicals and cell culture media were from Sigma-Aldrich (St. Louis, MO) and other culture reagents from Life Technologies (Carlsbad, CA), unless otherwise stated.
Rabbit anti-mouse P2X7 antibody (#APR-004) was obtained from Alomone labs (Jerusalem, Israel); beta-actin (AC-15, #ab6276) from Abcam (Cambridge, MA); Flotillin-2 (B6, #sc-28320) from Santa Cruz Biotechnology, Inc. (Dallas, TX); Caveolin-1 (7C8, #NB100-615) from Novus Biologicals (Littleton, CO); phospho-AKT (S473) (#9271), phospho-PRAS40 (T246) (#2997), phospho-S6K (T389) (#9205), phospho-S6 (S235/236) (#2211) from Cell Signaling Technology (Danvers, MA); HRP-conjugated goat anti-mouse (#31438), donkey anti-rabbit (#31458) and mouse anti-goat IgG (#31400) and the SuperSignal West Femto Maximum Sensitivity Substrate reagents (#PI-34096) were from Thermo Scientific (Rockford, IL).

de Andrade Mello P., Bian S., Savio L.E., Zhang H., Zhang J., Junger W., Wink M.R., Lenz G., Buffon A., Wu Y, & Robson S.C. (2017). Hyperthermia and associated changes in membrane fluidity potentiate P2X7 activation to promote tumor cell death. Oncotarget, 8(40), 67254-67268.

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