Spodoptera frugiperda sf9 insect cells

Manufactured by American Type Culture Collection

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Spodoptera frugiperda Sf9 insect cells are a common cell line used in protein expression studies. These cells are derived from the ovarian tissue of the fall armyworm, Spodoptera frugiperda, and are widely used for the production of recombinant proteins. Sf9 cells provide a eukaryotic expression system that is capable of post-translational modifications, making them a valuable tool for researchers in various fields.

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Lab products found in correlation

Minimal eagles medium mem, by Corning (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Vero e6 cell, by American Type Culture Collection (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Gemini Bio (1 mentions) Sf900ii medium, by Thermo Fisher Scientific (1 mentions)

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Sf9 cells (32 citations) Sf9 insect cells (9 citations)

2 protocols using spodoptera frugiperda sf9 insect cells

Vero E6 and Sf9 Cell Culture for SARS-CoV and MERS-CoV

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Vero E6 cells were originally obtained from the American Type Culture Collection (ATCC) and maintained in Minimal Eagles Medium (MEM; Corning) supplemented with 10% heat-inactivated Fetal Bovine Serum (FBS; SAFC), 1% l-Glutamine (Gibco) and 1% Penicillin/Streptomycin (Gemini Bio-Products). Spodoptera frugiperda Sf9 insect cells (ATCC CRL-1711) were maintained as suspension cultures in HyQ-SFX insect serum free medium (HyClone, Logan, UT) at 27 ± 2 °C.
Mouse adapted SARS-CoV (MA15) has been previously described [38] (link) and was grown in Vero E6 cells and stored at −80 °C. MERS-CoV (Jordan) was obtained from the NIH in conjunction with AFHSC-GEIS and NAMRU-3, with special assistance from Dr. Mohareb. All experiments with live virus were performed under biosafety level 3 conditions at the University of Maryland, Baltimore.
MERS-CoV (Jordan) Spike protein is 99.8% identical to the MERS-CoV (Al Hassa1) Spike protein sequence used in the nanoparticle cloning. We consider MERS-CoV (Al Hassa1) and MERS-CoV (Jordan) to be homologous in their Spike proteins. SARS-CoV (Urbani) Spike protein is 99.9% identical to the SARS-CoV (MA15) Spike protein sequence used in the nanoparticle cloning. We consider SARS-CoV (Urbani) and SARS-CoV (MA15) Spike proteins to be homologous.

Coleman C.M., Liu Y.V., Mu H., Taylor J.K., Massare M., Flyer D.C., Glenn G.M., Smith G.E, & Frieman M.B. (2014). Purified coronavirus spike protein nanoparticles induce coronavirus neutralizing antibodies in mice. Vaccine, 32(26), 3169-3174.

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Recombinant H5 Influenza Vaccine Evaluation

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Spodoptera frugiperda Sf9 insect cells (CRL-1711; ATCC, Manassas, VA, USA) were maintained in serum-free SF900II medium (Invitrogen, Carlsbad, CA, USA) at 27°C and used for production of recombinant baculoviruses (rBVs) and VLPs. Two HPAI strains (ZJ and DT) used as challenge virus were originated from clade 2.3.4.6 (A/Chicken/Zhejiang/2011, ZJ, H5N2) and clade 7 (A/Chicken/Huadong/4/2008, DT, H5N1). In this study, the recombinant Re6 commercial vaccines, and chicken sera against monovalent Re5, Re6, Re7, and Re8 antigens were purchased from Weike Biotechnology Co., Ltd., Harbin, China. Four commercial recombinant vaccines of Re5, Re6, Re7, and Re8 were inactivated whole-virus-based mineral oil emulsion vaccines (Weike). The six inner genes of four recombinant vaccine strains are derived from A/Puerto Rico/8/1934 virus (PR8, H1N1). The HA and NA genes of Re5–Re8 are derived from different subclades of H5 wild-type viruses, Re5 from strain of A/duck/Anhui/1/2006 (clade 2.3.4) (8 (link)), Re6 from virus of A/duck/Guangdong/S1322/2006 (clade 2.3.2) (8 (link)), Re7 from strain of A/Chicken/Liaoning/S4092/2011 (clade 7.2) (13 (link)), and Re8 from A/chicken/Guizhou/4/13 (clade 2.3.4.4) (14 (link)).

Wu P., Lu J., Zhang X., Mei M., Feng L., Peng D., Hou J., Kang S.M., Liu X, & Tang Y. (2017). Single Dose of Consensus Hemagglutinin-Based Virus-Like Particles Vaccine Protects Chickens against Divergent H5 Subtype Influenza Viruses. Frontiers in Immunology, 8, 1649.

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