In this study, three different brain cancer cell lines and two different pancreas cancer cell lines were used. Glioblastoma brain cancer cell lines U87-GBM (ATCC, #HTB-14), LN229-GBM (ATCC, #CRL-2611), T98G-GBM (ATCC, #CRL-1690), and pancreas cancer cell lines PANC-1 (ATCC, #CRL-1469), ASPC-1 (ATCC, #CRL-1682) cell lines were purchased from ATCC (U.S.A.). Then the cells were grown and expanded in DMEM (Gibco) medium with 10% fetal bovine serum (Gibco), 1% antibiotics (penicillin/streptomycin) at 37°C in 5% CO2 incubator. The cells were then removed from the flask with Trypsin/EDTA 0.25% (Gibco) and seeded at a density of 5x103 cells/well into 96 black well plates (Corning) for cell viability assays that measures metabolically active cells.
Ln229 gbm
Manufactured by American Type Culture Collection
Sourced in Japan
The LN229-GBM is a cell line derived from a human glioblastoma multiforme (GBM) tumor. It is a commonly used model for studying glioblastoma biology and evaluating potential therapeutic approaches.
Automatically generated - may contain errors
Lab products found in correlation
U87 gbm, by American Type Culture Collection (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Panc 1, by American Type Culture Collection (1 mentions)
Aspc 1, by American Type Culture Collection (1 mentions)
Htb 14, by American Type Culture Collection (1 mentions)
Skov3, by American Type Culture Collection (1 mentions)
Mcf 7, by American Type Culture Collection (1 mentions)
2 protocols using ln229 gbm
1
Comparative Analysis of Brain and Pancreas Cancer Cell Lines
2
Cytotoxicity Assay of Human Cell Lines
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LN229-GBM (ATCC, CRL-2611), T98G (ATTC, CRL-1690), U87 (ATCC, HTB-14), MCF7 (ATCC, HTB-22), SKOV3 (ATCC, HTB-77), A549 (ATCC, CCL-185) and Primary Human Mesenchymal Stem (hMSC) (UE7T-13 cells no. RBRC-RCB2161; RIKEN, Japan) cells were available in our laboratory. In vitro experiments were conducted using Gibco brand fetal bovine serum (FBS), high and low glucose Dulbecco’s Modified Eagle Medium (DMEM), Penicillin-Streptomycin, L-Glutamine, and Trypsin/EDTA 0.25%. Cytotoxicity assays were performed using the Promega brand CellTiter-Glo® Luminescent Cell Viability Assay (Cat. no. #G7572) and the Corning 96-black plate (Cat. no. #3603).
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