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P 97 puller

Manufactured by Drummond
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The P-97 puller is a laboratory instrument designed for pulling and forming glass micropipettes. It utilizes a heating element and precise control mechanisms to heat and then pull a glass capillary tube, resulting in the creation of fine-tipped glass micropipettes. The core function of the P-97 puller is to produce customized micropipettes for various scientific and experimental applications.

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Lab products found in correlation

Glass micropipette, by Drummond (2 mentions) Smiths medical pm, by Smith & Nephew (2 mentions) Cunningham adaptor, by Harvard Apparatus (2 mentions)

2 protocols using p 97 puller

1

Stereotaxic AAV delivery of fluorescent biosensors

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
PCR amplified roGFP, mitoroGFP, truncated MAO-B lacking C-terminus tail sequence required for anchoring (Amino acids 492–520), Perceval HR (Addgene plasmid #49082) 12 (link), or roGFP with the C-terminal anchoring sequence of MAO-B were subcloned into EcoRI and SalI restriction sites of the pFB-TH-SV40 vector and packaged into rAAVs using serotype 9 with titers 2.1-x1013 viral genome copies/ml (Virovek). Mice were anesthetized using an isoflurane precision vaporizer (Smiths Medical PM) and placed in a stereotaxic frame (David Kopf Instruments) with a Cunningham adaptor (Harvard Apparatus) to maintain anesthesia delivery throughout surgery. After exposing the skull, a small hole was drilled and 350 nL of viral vector delivered via a glass micropipette (Drummond Scientific Company) pulled on a Sutter P-97 puller. The substantia nigra pars compacta (SNc) was targeted at the following coordinates: AP: −3.05, ML: 1.20, and DV −4.30. All surgeries were performed in wild-type or MAO-A/B knockout mice. Experiments in animals with stereotaxic delivery of AAV viral vectors were performed after at least 10 days post-op.
Graves S.M., Xie Z., Stout K.A., Zampese E., Burbulla L.F., Shih J.C., Kondapalli J., Patriarchi T., Tian L., Brichta L., Greengard P., Krainc D., Schumacker P.T, & Surmeier D.J. (2019). Dopamine metabolism by a monoamine oxidase mitochondrial shuttle activates the electron transport chain. Nature neuroscience, 23(1), 15-20.
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2

Stereotaxic AAV delivery of fluorescent biosensors

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
PCR amplified roGFP, mitoroGFP, truncated MAO-B lacking C-terminus tail sequence required for anchoring (Amino acids 492–520), Perceval HR (Addgene plasmid #49082) 12 (link), or roGFP with the C-terminal anchoring sequence of MAO-B were subcloned into EcoRI and SalI restriction sites of the pFB-TH-SV40 vector and packaged into rAAVs using serotype 9 with titers 2.1-x1013 viral genome copies/ml (Virovek). Mice were anesthetized using an isoflurane precision vaporizer (Smiths Medical PM) and placed in a stereotaxic frame (David Kopf Instruments) with a Cunningham adaptor (Harvard Apparatus) to maintain anesthesia delivery throughout surgery. After exposing the skull, a small hole was drilled and 350 nL of viral vector delivered via a glass micropipette (Drummond Scientific Company) pulled on a Sutter P-97 puller. The substantia nigra pars compacta (SNc) was targeted at the following coordinates: AP: −3.05, ML: 1.20, and DV −4.30. All surgeries were performed in wild-type or MAO-A/B knockout mice. Experiments in animals with stereotaxic delivery of AAV viral vectors were performed after at least 10 days post-op.
Graves S.M., Xie Z., Stout K.A., Zampese E., Burbulla L.F., Shih J.C., Kondapalli J., Patriarchi T., Tian L., Brichta L., Greengard P., Krainc D., Schumacker P.T, & Surmeier D.J. (2019). Dopamine metabolism by a monoamine oxidase mitochondrial shuttle activates the electron transport chain. Nature neuroscience, 23(1), 15-20.
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