T4 gt7 phage dna

Plasmid DNA (luciferase T7 control DNA, 4331 bp) containing a gene encoding luciferase and a T7 RNA polymerase promoter sequence was purchased from Promega (Madison, WI, USA). T4 GT7 phage DNA (166 kbp; contour length, 57 µm) was purchased from Nippon Gene (Toyama, Japan). The fluorescent cyanine dye YOYO-1 (1,10-(4,4,7,7-tetramethyl-4,7-diazaundecamethylene)-bis-4-(3-methyl-2,3-dihydro-(benzo-1,3-oxazole)-2-methylidene)-quinolinium tetraiodide) was purchased from Molecular Probes Inc. (Eugene, OR, USA). The antioxidant 2-mercaptoethanol was purchased from Wako Pure Chemical Industries (Osaka, Japan). Cisplatin was synthesized according to a previous report [70 (link)]. Transplatin was purchased from Sigma Aldrich (Saint Louis, MO, USA). Other chemicals were of analytical grade.

Spermidine trihydrochloride (SPD) was purchased from Nacalai Tesque (Kyoto, Japan). Bis(3-aminopropyl)amine (NSPD) was purchased from Sigma-Aldrich (St. Louis, MO, USA). The fluorescent cyanine dye YOYO-1 (1,10-(4,4,8,8-tetramethyl-4,8-diazaundecamethylene)bis[4-[(3-methylbenzo-1,3-oxazol-2-yl)methylidene]-l,4-dihydroquinolinium] tetraiodide) was purchased from Molecular Probes, Inc. (Eugene, OR, USA). The antioxidant 2-mercaptoethanol (2-ME) and calf thymus DNA (CT DNA: 8–15 kbp) were purchased from Wako Pure Chemical Industries (Osaka, Japan). T4 GT7 phage DNA (contour length: 57 μm, 166 kbp) was purchased from Nippon Gene (Toyama, Japan). Plasmid DNA (Luciferase T7 Control DNA, 4331 bp) containing a T7 RNA polymerase promotor sequence was purchased from Promega (Madison, WI, USA). Other chemicals obtained from commercial sources were of analytical grade.