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Gentle macs agitator

Manufactured by Miltenyi Biotec
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The Gentle MACS Agitator is a laboratory equipment designed for gentle cell dissociation and homogenization. It provides a controlled and uniform mixing motion to facilitate the processing of biological samples.

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Lab products found in correlation

Lsrfortessa sorp 1 flow cytometer, by BD (4 mentions) Collagenase type 4, by Merck Group (2 mentions) Live dead fixable blue dead cell stain kit, by Thermo Fisher Scientific (2 mentions) Tumor dissociation kit, by Miltenyi Biotec (2 mentions)

4 protocols using gentle macs agitator

1

Isolation and Analysis of Pancreatic Tumor Cells

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Harvested pancreatic tumors were rinsed with PBS, minced with a scalpel, and digested using 1mg/ml of type IV collagenase (Sigma-Aldrich, San Jose, CA) and a Gentle Macs Agitator (Miltenyi Biotec, San Diego, CA). Tumor digests were passed through a 70μm filter, washed in PBS, and stained for flow cytometry analysis with antibodies listed in Supplemental Table S1. Stained cells were washed with FACS buffer prior to sample acquisition with the BD LSRFortessa SORP I flow cytometer (BD Bioscience). Flow cytometry data was analyzed using FlowJo software (TreeStar, Ashland, OR).
Li D., Schaub N., Guerin T.M., Bapiro T.E., Richards F.M., Chen V., Talsania K., Kumar P., Gilbert D.J., Schlomer J.J., Kim S.J., Sorber R., Teper Y., Bautista W., Palena C., Ock C.Y., Jodrell D.I., Pate N., Mehta M., Zhao Y., Kozlov S, & Rudloff U. (2021). T Cell–Mediated Antitumor Immunity Cooperatively Induced By TGFβR1 Antagonism and Gemcitabine Counteracts Reformation of the Stromal Barrier in Pancreatic Cancer. Molecular Cancer Therapeutics, 20(10), 1926-1940.
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2

Tumor Dissociation and Flow Cytometry

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Pancreatic tumors were harvested, washed with PBS, minced with a scalpel, and digested using Tumor Dissociation Kit, mouse with a Gentle Macs Agitator (Miltenyi Biotec). Tumor lysates were passed through a 70-μm filter, washed in PBS, and stained with the Live/Dead Fixable Blue Dead Cell Stain Kit (Thermo Fisher Scientific) and antibodies listed in Supplementary Table S1. Stained cells were washed with FACS buffer before sample acquisition with the BD LSRFortessa SORP I flow cytometer (BD Biosciences). Flow cytometry data were analyzed using FlowJo software (TreeStar).
Li D., Miermont A.M., Sable R., Quadri H.S., Mathews Griner L.A., Martin S.E., Odzorig T., De S., Ferrer M., Powers A.S., Hewitt S.M, & Rudloff U. (2023). Scaffolding Protein Connector Enhancer of Kinase Suppressor of Ras 1 (CNKSR1) Regulates MAPK Inhibition Responsiveness in Pancreas Cancer via Crosstalk with AKT Signaling. Molecular Cancer Research, 21(4), 316-331.
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3

Isolation and Characterization of Pancreatic Tumor Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Pancreatic tumors were harvested, washed with PBS, minced with a scalpel, and digested using Tumor Dissociation Kit, mouse with a Gentle Macs Agitator (Miltenyi Biotec, San Diego, CA). Tumor lysates were passed through a 70μm filter, washed in PBS, and stained with the Live/Dead Fixable Blue Dead Cell Stain Kit (ThermoFisher Scientific) and antibodies listed in Supplementary Table 1. Stained cells were washed with FACS buffer prior to sample acquisition with the BD LSRFortessa SORP I flow cytometer (BD Bioscience). Flow cytometry data was analyzed using FlowJo software (TreeStar, Ashland, OR).
Li D., Miermont A.M., Sable R., Quadri H.S., Mathews Griner L.A., Martin S.E., Odzorig T., De S., Ferrer M., Powers A.S., Hewitt S.M, & Rudloff U. (2023). Scaffolding Protein Connector Enhancer of Kinase Suppressor of Ras 1 (CNKSR1) Regulates MAPK Inhibition Responsiveness in Pancreas Cancer via Crosstalk with AKT Signaling. Molecular Cancer Research, 21(4), 316-331.
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4

Dissociation and Analysis of Pancreatic Tumors

Check if the same lab product or an alternative is used in the 5 most similar protocols
Harvested pancreatic tumors were rinsed with PBS, minced with a scalpel, and digested using 1 mg/mL of type IV collagenase (Sigma-Aldrich) and a Gentle Macs Agitator (Miltenyi Biotec). Tumor digests were passed through a 70-μm filter, washed in PBS, and stained for flow cytometry analysis with antibodies listed in Supplementary Table S1. Stained cells were washed with FACS buffer prior to sample acquisition with the BD LSRFortessa SORP I flow cytometer (BD Biosciences). Flow cytometry data was analyzed using FlowJo software (TreeStar).
Li D., Schaub N., Guerin T.M., Bapiro T.E., Richards F.M., Chen V., Talsania K., Kumar P., Gilbert D.J., Schlomer J.J., Kim S.J., Sorber R., Teper Y., Bautista W., Palena C., Ock C.Y., Jodrell D.I., Pate N., Mehta M., Zhao Y., Kozlov S, & Rudloff U. (2021). T Cell–Mediated Antitumor Immunity Cooperatively Induced By TGFβR1 Antagonism and Gemcitabine Counteracts Reformation of the Stromal Barrier in Pancreatic Cancer. Molecular Cancer Therapeutics, 20(10), 1926-1940.
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