Vacuette serum gel tubes

Manufactured by Greiner

Sourced in Austria

Vacuette serum gel-tubes are laboratory equipment used for the collection and processing of blood samples. They contain a gel barrier that separates the serum from the cellular components after centrifugation.

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Lab products found in correlation

Xp 300 analyzer, by Sysmex (2 mentions) Edta tube, by Greiner (2 mentions) Cxcl10, by Thermo Fisher Scientific (1 mentions) Tnf α, by Thermo Fisher Scientific (1 mentions) Immulite 2000 xpi analyzer, by Siemens (1 mentions) Immulite 2000 xpi, by Siemens (1 mentions)

Spelling variants of this product

Vacuette (368 citations) Vacuette tubes (90 citations) Vacuette® serum tubes (4 citations) Vacuette gel tubes (2 citations)

6 protocols using vacuette serum gel tubes

Microbial Exposure and Serum Analysis

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The research material consisted of 70 serum samples from building users, who resided in microbe-damaged buildings, and 89 serum samples from building users from non-damaged buildings (Table 1).
The blood samples were collected from the building users in 8 ml Vacuette serum gel-tubes (Greiner Bio-One, Kremsmünster, Austria). Serum was prepared by centrifugation at 540 g for 10 min and then distributed into 2 ml Eppendorf tubes and stored at −80°C. Samples were collected while the individuals worked or lived in the buildings.

Atosuo J., Karhuvaara O., Suominen E., Vilén L., Nuutila J, & Putus T. (2020). Indoor-related microbe damage induces complement system activation in building users. Innate Immunity, 27(1), 15-22.

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Cytokine and Chemokine Profiling

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PB samples were collected from patients and healthy individuals into VACUETTE Serum Gel tubes (Greiner-Bio-One, Kremsmünster, Austria) and were centrifuged for 10 min at 2000 g. Subsequently, serum samples were subdivided into small aliquots to be stored at − 80 °C until tested for cytokine and chemokines levels. ELISA kits were used to determine monocyte chemotactic protein-1 (MCP-1 or CCL2, Thermo Scientific), MIP-3a (CCL20, Thermo Scientific), IP-10 (CXCL10, Thermo Scientific) and TNFα (Thermo Scientific) serum levels, according to the manufacturer’s instructions.

Martín-Sierra C., Martins R., Coucelo M., Abrantes A.M., Oliveira R.C., Tralhão J.G., Botelho M.F., Furtado E., Domingues M.R., Paiva A, & Laranjeira P. (2020). Elevated soluble TNFα levels and upregulated TNFα mRNA expression in purified peripheral blood monocyte subsets associated with high-grade hepatocellular carcinoma. Journal of Inflammation (London, England), 17, 14.

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COVID-19 Sampling and Antibody Testing

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Nasopharyngeal sample collection kits (Copan Diagnostics, Murrieta, CA, USA), consisting of a flexible minitip flocked swab in a tube filled with 1 mL Universal Transport Medium (UTM), were used for COVID-19 sample collection in all 70 returning service members, immediately upon arrival in Belgium (D0) and two weeks later (D14). Blood samples were collected, using VACUETTE serum gel tubes (Greiner Bio-One, Vilvoorde, Belgium), for COVID-19 antibody testing in all returning personnel on D0 and D14. Blood was allowed to clot for a minimum of 30 min in a vertical position and then centrifuged at room temperature in a swinging bucket rotor for 10 min at 1500 g. Serum samples were tested immediately and stored at −80 °C ± 5 °C for possible re-testing.

Pirnay J.P., Selhorst P., Cochez C., Petrillo M., Claes V., Van der Beken Y., Verbeken G., Degueldre J., T’Sas F., Van den Eede G., Weuts W., Smets C., Mertens J., Geeraerts P., Ariën K.K., Neirinckx P, & Soentjens P. (2020). Study of a SARS-CoV-2 Outbreak in a Belgian Military Education and Training Center in Maradi, Niger. Viruses, 12(9), 949.

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Fasted Blood Analysis for Hemoglobin, Ferritin, and Vitamin D

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Morning fasted blood samples were obtained from the antecubital vein for the analyses of hemoglobin, ferritin, and serum total 25-hydroxyvitamin D, e.g., serum 25(OH)D. Blood for the hemoglobin analysis was drawn into EDTA tubes (Greiner-Bio-One GmbH, Kremsmünster, Austria) and immediately further analyzed with Sysmex XP300 analyzer (SysmexCo., Kobe, Japan). For serum ferritin, the blood was drawn into Vacuette gel serum tubes (Greiner-Bio-One GmbH, Kremsmünster, Austria) and centrifuged for 10 min with 3,600 rpm to collect serum, which was then frozen to −20°C for further analysis. The samples were analyzed with Siemens Immulite 2000 XPI analyzer (Siemens Healthcare Lianberis, United Kingdom), where the serum ferritin was determined by using immunometric chemiluminescence method. The sensitivity of the assay for ferritin was 0.4 µ/L and the precision (CV%) for the assay was 4.6%. The measurements of serum 25(OH)D were performed using electrochemiluminescence immunoassays (ECLIA).

Mishica C., Kyröläinen H., Valtonen M., Holmberg H.C, & Linnamo V. (2023). Performance-related physiological changes induced by one year of endurance training in young athletes. Frontiers in Sports and Active Living, 5, 1149968.

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Fasting Blood Hormone Analysis Protocol

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Fasting blood samples were obtained from the antecubital vein for analysis of serum hormone concentrations. M₁ and M₂ samples from each participant were collected at the same time of day between 7 a.m. and 9 a.m. Blood was drawn into Vacuette gel serum tubes (Greiner-Bio-One GmbH, Kremsmünster, Austria). The tubes were centrifuged at 3600 rpm for 10 min to collect serum, which was frozen at -20°C. Concentrations of insulin, cortisol, insulin-like growth factor 1 (IGF-1), free triiodothyronine (T3), free thyroxine (T4), thyroid-stimulating hormone (TSH), leptin, and testosterone were analyzed by an immunometric chemiluminescence method (Immulite 2000 ×Pi, Siemens Healthcare, United Kingdom). The assay sensitivities were 2.0 U·L⁻¹ for insulin, 5.5 nmol·L⁻¹ for cortisol, 2.6 nmol·L⁻¹ for IGF-1, 1.5 pmol·L⁻¹ for free T3, 1.4 for free T4, 0.004 mU·L⁻¹ for TSH, 0.2 ug·L⁻¹ for leptin, and 0.5 nmol·L⁻¹ for testosterone. Reliabilities expressed as a coefficient of variation were 8.8% for insulin, 7.7% for cortisol, 4.4% for IGF-1, 10.3% for free T3, 6.6% for free T4, 9.2% for TSH, 4.9% for leptin, and 10.9% for testosterone.

Kettunen O., Mikkonen R., Linnamo V., Mursu J., Kyröläinen H, & Ihalainen J.K. (2023). Nutritional intake and anthropometric characteristics are associated with endurance performance and markers of low energy availability in young female cross-country skiers. Journal of the International Society of Sports Nutrition, 20(1), 2226639.

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Fasting Blood Biomarkers Analysis

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Fasting blood samples were obtained from an antecubital vein for analysis of hemoglobin, cortisol, leptin, triiodothyronine (T3), insulin, insulin-like growth factor 1 (IGF-1), and glucose. PRE and POST samples from each subject were collected at the same time of day between 7am and 9am. Blood was drawn into EDTA tubes (Greiner-Bio-One GmbH, Kremsmünster, Austria) for analyses of hemoglobin that were completed immediately with Sysmex XP300 analyzer (SysmexCo., Kobe, Japan). For the determination of serum hormone concentrations, blood was drawn into Vacuette gel serum tubes (Greiner-Bio-One GmbH, Kremsmünster, Austria) and centrifuged at 3600 rpm for 10 min to collect serum, which was frozen at -20ºC. Concentrations of cortisol, leptin, T3, insulin, and IGF-1 were analyzed by an immunometric chemiluminescense method (Immulite 2000 XPi, Siemens Healthcare, United Kingdom). The assay sensitivities were 5.5 nmol • L -1 (cortisol), 8.2 ug • L -1 (leptin), 1.5 pmol • L -1 (T3), 2 U • L -1 (insulin), 1.7 nmol • L -1 (IGF-1), and 2.0 mmol

Kettunen O., Ihalainen J.K., Ohtonen O., Valtonen M., Mursu J., & Linnamo V. (2021). Energy availability during training camp is associated with signs of overreaching and changes in performance in young female cross-country skiers. Biomedical Human Kinetics, 13(1), 246-254.

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