Bond rx automated system

Manufactured by Leica Biosystems

Sourced in Germany

The Bond RX is an automated staining system designed for immunohistochemistry (IHC) and in situ hybridization (ISH) assays. It automates the entire process from slide preparation to staining and detection, providing consistent and reproducible results.

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Lab products found in correlation

Aperio image analysis software, by Leica Biosystems (1 mentions) Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions) Anti prb, by Cell Signaling Technology (1 mentions) Bond polymer refine kit, by Leica Biosystems (1 mentions) Sc 8018, by Santa Cruz Biotechnology (1 mentions) Er1 buffer, by Leica Biosystems (1 mentions) Anti cd3 antibody, by Agilent Technologies (1 mentions) Sodium citrate based er1 buffer, by Leica Biosystems (1 mentions) Anti iba1 antibody, by Fujifilm (1 mentions)

3 protocols using bond rx automated system

Biomarker Stability in Glioblastoma Progression

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To test the stability of proposed pharmacodynamic biomarkers (pRB, pFOXM1, MIB1, and cleaved caspase-3), we analyzed a historical cohort of 10 matched primary and recurrent glioblastoma patients who received standard-of-care Stupp regimen and were not enrolled in the study. FFPE tissues were stained with anti-pRB (Cell Signaling, #8516, 1:400), anti-pFOXM1 (Cell Signaling, #14655, 1:200), anti-MIB1 (DAKO, M724029, 1:100), and anti-cleaved caspase-3 (Cell Signaling, #9661, 1:300) using our standardized immunohistochemistry protocol with the BOND RX automated system (Leica Biosystems, Wetzlar, Germany). The stained slides were imaged and analyzed by a board-certified pathologist, and Aperio Image analysis software (Leica Biosystems) was used to assess differences in positivity for pRB, MIB1, and cleaved caspase-3 in primary vs. recurrent tumors. There were no significant differences between primary and recurrent tissues in the levels of the tested biomarkers.

Tien A.C., Li J., Bao X., Derogatis A., Kim S., Mehta S, & Sanai N. (2019). A Phase 0 Trial of Ribociclib in Recurrent Glioblastoma Patients Incorporating a Tumor Pharmacodynamic- and Pharmacokinetic-Guided Expansion Cohort. Clinical cancer research : an official journal of the American Association for Cancer Research, 25(19), 5777-5786.

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Immunostaining of SARS-CoV-2-Infected Lung Tissue

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The 10% neutral buffered formalin–fixed lung sections of SARS-CoV-2–infected and control animals were immunostained with anti–ionized calcium binding adaptor molecule-1 (Iba-1) antibody (1:1000; 019-19741; Wako, Richmond, VA), anti-CD3 antibody (1:200; reference A0452; Dako, Santa Clara, CA), or anti–pan-cytokeratin antibody (1:1000; sc-8018; Santa Cruz, Dallas, TX). Heat-induced epitope retrieval was conducted by heating slides to 95°C for 20 minutes in sodium citrate–based ER1 buffer (Leica Biosystems) before immunostaining. Dual immunostaining for the epithelial marker pan-cytokeratin and the macrophage marker Iba-1 was performed on lung sections of SARS-CoV-2–infected animals. For epitope retrieval, slides were heated to 100°C in sodium citrate–based ER1 buffer for 20 minutes (Leica Biosystems). Slides were then stained with anti–Iba-1 antibody (1:2000; 019-19741; Wako) using the Bond Polymer Refine Kit (catalog number DS9800; Leica Biosystems). The slides were stained using a pan-cytokeratin antibody (1:1000; sc-8018; Santa Cruz) with the Bond Polymer Refine Red Kit (catalog number DS9390; Leica Biosystems). Immunostaining was performed using the Bond RX automated system (Leica Biosystems). Positive immunostaining was visualized using diaminobenzidine and Bond Red detection kit, and slides were counterstained with hematoxylin.

Mulka K.R., Beck S.E., Solis C.V., Johanson A.L., Queen S.E., McCarron M.E., Richardson M.R., Zhou R., Marinho P., Jedlicka A., Guerrero-Martin S., Shirk E.N., Braxton A.M., Brockhurst J., Creisher P.S., Dhakal S., Brayton C.F., Veenhuis R.T., Metcalf Pate K.A., Karakousis P.C., Zahnow C.A., Klein S.L., Jain S.K., Tarwater P.M., Pekosz A.S., Villano J.S, & Mankowski J.L. (2022). Progression and Resolution of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Infection in Golden Syrian Hamsters. The American Journal of Pathology, 192(2), 195-207.

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TREM2 and IBA1 Expression in SIV-Infected Brain

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TREM2 ISH was combined with IBA1 immunostaining performed on frontal cortex sections from an SIV infected animal. TREM2 ISH was performed as described above. The first heat-induced epitope retrieval was conducted by heating slides to 95°C in EDTA-based ER2 buffer for 15 minutes (Leica Biosystems, Richmond, IL). Slides were then stained with anti-IBA1 antibody (cat. 01919741, Wako, Richmond, VA) at a dilution of 1:500. IHC was performed using the Bond RX automated system (Leica Biosystems, Richmond, IL). A second heat-induced epitope retrieval was conducted by heating slides to 95°C for 20 minutes in sodium citrate-based ER1 buffer (Leica Biosystems, Richmond, IL). Positive immunostaining was visualized using DAB, and slides were counterstained with hematoxylin.

Knight A.C., Brill S.A., Solis C.V., Richardson M.R., McCarron M.E., Queen S.E., Bailey C.C, & Mankowski J.L. (2020). Differential Regulation of TREM2 and CSF1R in CNS Macrophages in an SIV/Macaque Model of HIV CNS Disease. Journal of neurovirology, 26(4), 511-519.

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