Gsdmd mice

Manufactured by Jackson ImmunoResearch

Gsdmd−/− mice are genetically engineered mice with a deletion of the Gsdmd gene. The Gsdmd gene encodes the Gasdermin D protein, which is involved in a form of cell death called pyroptosis. These mice can be used to study the role of Gasdermin D and pyroptosis in various biological processes.

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4 protocols using gsdmd mice

Genetically Engineered Mouse Models

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C57BL/6J (RRID:IMSR_JAX:000664), Balb/cJ (RRID: IMSR_JAX:000651), Batf3^−/− (RRID: IMSR_JAX:013755), and Rag1^−/− (RRID: IMSR_JAX:002216), NSG (RRID: IMSR_JAX:005557), Sting^gt (RRID: IMSR_JAX:017537), IL10^−/− mice (RRID: IMSR_JAX:002251), IL10rb^−/− mice (RRID: IMSR_JAX:005027), Gsdmd^−/− mice (RRID: JAX032663) and Casp1/11^−/− mice (RRID: JAX016621) were obtained from The Jackson Laboratory. Havcr2^−/− mice were gifted by Dr. Binfeng Lu, and Timd4^−/− mice were gifted from Dr. Vijay K. Kuchroo. All mice were maintained and bred under in specific pathogen-free conditions animal facilities at UPMC Hillman Cancer center. All mice were used for experiments at 8-10 weeks of age (age and sex matched, both sexes used). All animal experiments were performed under protocols approved by University of Pittsburgh Animal Care and Use Committee. All mice were housed in specific-pathogen-free conditions at an ambient temperature of 20–26°C and humidity of 30–70% with a 12 h:12 h light:dark cycle before use.

Wang W., Wu S., Cen Z., Zhang Y., Chen Y., Huang Y., Cillo A.R., Prokopec J.S., Quarato G., Vignali D.A., Stewart-Ornstein J., Li S., Lu B, & Gong Y.N. (2022). Mobilizing phospholipids on tumor plasma membrane implicates phosphatidylserine externalization blockade for cancer immunotherapy. Cell reports, 41(5), 111582.

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Genetically Engineered Mouse Models

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Schistosome Infection Model in Mice

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Five- to six-week-old female C57BL/6, and GSDMD^−/− mice were purchased from The Jackson Laboratory and Swiss Webster mice from Taconic Biosciences. Caspase-1^−/−, RIP3^−/− and RIP3^−/−/Casp8^−/−, ASC^−/−, NLRP3^−/− and AIM2^−/− mice were obtained from Dr. Katherine Fitzgerald. All mice were maintained at the Pennsylvania State University’s and the Tufts University School of Medicine’s Animal Facilities in accordance with the Institutional Animal Care and Use Committee (IACUC) and the Association for Assessment and Accreditation of Laboratory Animal Care guidelines. Schistosome-infected Biomphalaria glabrata snails were provided by the NIAID Schistosomiasis Resource Center at the Biomedical Research Institute (Rockville, MD) through NIH-NIAID Contracts HHSN272201700014I (to PK) and HHSN272201000005I (to MS) for distribution through the BEI Resources Repository. Live schistosome eggs were isolated from livers of infected Swiss Webster mice under sterile conditions by a series of blending and straining techniques, as previously described (8 (link)).

Suresh Kumar Meena Kumari M., Liu P., Jump K., Morales Y., Miller E.A., Shecter I., Stadecker M.J, & Kalantari P. (2024). NLRP3 and AIM2 inflammasomes exacerbate the pathogenic Th17 cell response to eggs of the helminth Schistosoma mansoni. bioRxiv.

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Schistosoma mansoni Infection in Genetically Engineered Mice

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Five- to six-week-old female CBA/J, C57BL/6, TRIF^−/−, IFNAR1^−/−, STAT1^−/−, and GSDMD^−/− mice were purchased from The Jackson Laboratory and Swiss Webster mice from Taconic Biosciences. cGAS^−/− mice were from Katherine Fitzgerald and STING^−/− mice were from Alexander Poltorak. Breeding colonies were established for STING^−/−, IFNAR1^−/−, and cGAS^−/− mice. All mice were maintained at the Tufts University School of Medicine Animal Facility in accordance with the Association for Assessment and Accreditation of Laboratory Animal Care guidelines. The mice were infected by i.p. injection with 85 cercariae of S. mansoni (Puerto Rico strain) shed from infected Biomphalaria glabrata snails. The snails were provided by the NIAID Schistosomiasis Resource Center of the Biomedical Research Institute (Rockville, MD) through NIH-NIAID Contract HHSN272201000005I for distribution through BEI Resources. Live schistosome eggs were isolated from livers of infected Swiss Webster mice under sterile conditions by a series of blending and straining techniques, as previously described (84 (link)).

Kalantari P., Shecter I., Hopkins J., Pilotta Gois A., Morales Y., Harandi B.F., Sharma S, & Stadecker M.J. (2023). The balance between gasdermin D and STING signaling shapes the severity of schistosome immunopathology. Proceedings of the National Academy of Sciences of the United States of America, 120(13), e2211047120.

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