Monomers diluted 1:20,000 in methanol were separated on an Agilent 1100 LC system equipped with a Zorbax Eclipse Plus C18 column (Agilent) using a mobile phase consisting of (A) 0.3% formic acid in water and (B) 0.3% formic acid in acetonitrile. A linear gradient of 50% B to 95% B was performed over 10 min at 50 °C. Separated samples were ionized by ESI followed by MS analysis on an Agilent MSD ion trap mass spectrometer.
Msd ion trap mass spectrometer
Manufactured by Agilent Technologies
Sourced in United States, Germany
The MSD ion trap mass spectrometer is a laboratory instrument used for the identification and quantification of chemical compounds. It utilizes ion trap technology to capture, isolate, and analyze ionized molecules based on their mass-to-charge ratio. The core function of this product is to provide high-sensitivity and high-resolution mass analysis capabilities for various applications in analytical chemistry and related fields.
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Lab products found in correlation
1100 lc system, by Agilent Technologies (2 mentions)
Zorbax eclipse plus c18 column, by Agilent Technologies (2 mentions)
Trypsin, by Agilent Technologies (1 mentions)
Zorbax 300sb c18, by Agilent Technologies (1 mentions)
1200 series, by Agilent Technologies (1 mentions)
Hp 3d ce system, by Agilent Technologies (1 mentions)
Ts 100 thermo shaker, by Biosan (1 mentions)
2002 potentiometer, by Crison (1 mentions)
Electrode 52 03, by Crison (1 mentions)
4 protocols using msd ion trap mass spectrometer
1
Monomers Separation and Identification
2
Proteomic Analysis of C. d. cumanensis
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The isolated fractions of C. d. cumanensis were alkylated, reduced, and digested with 0.1 ng trypsin (Agilent Technologies, Santa Clara, CA, USA) at 30 °C overnight. The products of the digestion were then injected into an LC/MS/MS system (1200 series, Agilent Technologies, Santa Clara, CA, USA) on a nano C-18 column (Agilent Zorbax 300SB-C18; 150 × 0.075 mm, 3.5 μm) at a flow of 0.2 μL/min and coupled to an MSD IonTrap mass spectrometer (6310 series, Agilent Technologies, Santa Clara, CA, USA). MS/MS mass spectra were obtained under the following conditions: positive mode, dynamic range from 200 to 1200 Da, electrospray at 2 kV, 230 °C drying temperature, and actuator trap at 200 μs. The ChemStation program G2070-91126 (Agilent Technologies, Santa Clara, CA, USA) was used for deconvolution of the MS/MS spectra in the loaded state.
3
Monomers Separation and Identification
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Monomers diluted 1:20,000 in methanol were separated on an Agilent 1100 LC system equipped with a Zorbax Eclipse Plus C18 column (Agilent) using a mobile phase consisting of (A) 0.3% formic acid in water and (B) 0.3% formic acid in acetonitrile. A linear gradient of 50% B to 95% B was performed over 10 min at 50 °C. Separated samples were ionized by ESI followed by MS analysis on an Agilent MSD ion trap mass spectrometer.
4
CE-MS Analysis of Biomolecule Samples
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All experiments were performed on an Agilent Technologies HP 3D CE system (Waldbronn, Germany) coupled to an MSD Ion Trap mass spectrometer (Agilent Technologies). An electrospray G1603A Agilent Technologies sprayer was used as a sheath-flow-CE-ESI-MS interface. The sheath liquid was delivered by an infusion pump KD Scientific 100 Series (Holliston, MA, USA). The detailed conditions affecting CE-MS have been discussed elsewhere, as has the tuning of the IT mass spectrometer [33] (link). pH measurements were performed with a Crison 2002 potentiometer and a Crison electrode 52-03 (Crison Instruments, Barcelona, Spain). Sample incubation was performed with a Thermo-Shaker TS-100 (Biosan, Warren, USA). Centrifugation was performed in a thermostated Rotanta 460 centrifuge (Hettich Zentrifugen, Tuttlingen, Germany).
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