The TMPRSS2 enzyme assay was performed using a modification of a previously published protocol [51 (link)]. The fluorogenic substrate, Boc-Gln-Ala-Arg-AMC · HCl, (Bachem, Torrance, CA. USA) was dissolved in DMSO and diluted in reaction buffer (50 mM Tris pH 8, 150 mM NaCl) to a final concentration of 10 μM. The fluorogenic substrate was added to each well of a 96-well plate and the fluorescence intensity was measured at 340/440 nm over a 1 hr period at 37 °C using a Bio-Tek Synergy HTX (Agilent, Santa Clara, CA, USA) plate reader.
Boc gln ala arg amc hcl
Manufactured by Bachem
Boc-Gln-Ala-Arg-AMC · HCl is a chemical compound used in various laboratory applications. It serves as a substrate for the measurement of enzymatic activity. The compound is composed of a tetrapeptide sequence (Boc-Gln-Ala-Arg) linked to the fluorescent dye aminomethylcoumarin (AMC). The hydrochloride (HCl) salt is provided for improved solubility and stability.
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3 protocols using boc gln ala arg amc hcl
1
TMPRSS2 Enzyme Fluorometric Assay
2
TMPRSS2 Enzyme Activity Assay
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The TMPRSS2 enzyme assay was performed using a modification of a previously published protocol52 (link). The fluorogenic substrate, Boc-Gln-Ala-Arg-AMC · HCl, (Bachem, Torrance, CA. USA) was dissolved in DMSO and diluted in reaction buffer (50 mM Tris pH 8, 150 mM NaCl) to a final concentration of 10 µM. The fluorogenic substrate was added to each well of a 96-well plate and the fluorescence intensity was measured at 340/440 nm over a 1 h period at 37 °C using a Bio-Tek Synergy HTX (Agilent, Santa Clara, CA, USA) plate reader.
3
TMPRSS2 Enzyme Assay Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The TMPRSS2 enzyme assay was performed using a previously published protocol, with some modification [24] . The fluorogenic substrate, Boc-Gln-Ala-Arg-AMC•HCl (Bachem, Torrance, CA. USA) was dissolved in DMSO and diluted in reaction buffer (50 mM Tris pH 8, 150 mM NaCl) to a final concentration of 10 µM. The fluorogenic substrate was added to each well of a 96-well plate, and fluorescence intensity was measured at 340/440 nm over 1 hr at 37 °C using a BioTek Synergy HTX (Agilent, Santa Clara, CA, USA).
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