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13 protocols using ethanol c2h6o

1

Synthesis and Characterization of Iron Oxide Nanoparticles

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Ferric chloride hexahydrate (FeCl3·6H2O), ethylene glycol anhydrous (C2H6O2), polyethylene glycol (PEG—C2nH4n+2On+1) with two different molecular weights (Mw = 8000 g/mol and Mw = 20,000 g/mol), ethanol (C2H6O), and RA (C18H16O8) were purchased from Sigma-Aldrich Merck (Darmstadt, Germany). Sodium acetate trihydrate (NaAc—C2H3NaO2·3H2O) was purchased from Silal Trading (Bucharest, Romania). Phosphate buffer saline (PBS) was purchased from Carl Roth (Karlsruhe, Baden-Württemberg, Germany). All chemicals were of analytical purity and used with no further purification.
The BHK and HepG2 cell lines were used from the collection of the Institute for Diagnosis and Animal Health (I.D.A.H.)
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2

Synthesis of Multicomponent Nanoparticles

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The following reagents from Sigma–Aldrich Co. (St Louis, MO, USA) were used to synthesize the NPs: sodium tetrachloropalladate(II) (Na2PdCl4, Cat. No. 379808), copper(II) acetylacetonate (Cu(acac)2, Cat. No. 514365), rhodium(III) acetylacetonate (Rh(acac)3, Cat. No. 282774), rhodium(III) chloride hydrate (RhCl3, Cat. No. 206261), d‐(−)‐ribose (C5H10O5, Cat. No. R7500), silica (SiO2, Cat. No. S5505), sodium borohydride (NaBH4, Cat. No. 452882), acetone (C3H6O, Cat. No. 650101), 1‐octadecene (ODE, C18H36, Cat. No. O806), and oleylamine (OAm, C18H37N, Cat. No. O7805). The following solvents were used to wash the NPs: hexane (C6H14, Cat. No. 32293, Sigma–Aldrich Co., St Louis, MO, USA) and ethanol (C2H6O, Cat. No. 10437341, Fisher Scientific U.K. Ltd, Loughborough, UK). Deionized (DI) water with a resistivity of 18.2 MΩ cm was used to prepare all aqueous solutions used in this study.
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3

Chitosan-PVA Hydrogel for Biomedical Applications

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Medium weight chitosan (Sigma Aldrich), PVA (87%–89% hydrolyzed, Sigma Aldrich), glycidyl trimethyl ammonium chloride (GTMAC, Sigma Aldrich), acetic acid (CH3COOH, Riedel-de Haen), sodium acetate (CH3COONa, Merck), sodium hydroxide (NaOH, Merck), hydrochloric acid (HCl, 37%, Merck), perchloric acid (HClO4, Isolab), potassium chromate (K2CrO4, Merck), silver nitrate (AgNO3, Sigma Aldrich), ethanol (C2H6O, Sigma Aldrich), glutaraldehyde (C5H8O2, 25%, Sigma Aldrich), phosphate buffered saline tablet (PBS, Sigma Aldrich), and C. officinalis flowers were commercially supplied and used without further purification.
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4

Synthesis and Characterization of Potassium Permanganate Composite

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Potassium permanganate
(KMnO4), sodium sulphate (Na2SO4),
cobalt nitrate [Co (NO3)2], urea, ethanol (C2H6O), and so on were purchased from Sigma Aldrich,
carbon black was purchased from Alfa Aesar, and N-Methyl-2 pyrrolidone (NMP) was obtained using high-performance liquid
chromatography. All the solvents and reagents are of analytical grade,
and the solution was prepared in distilled water.
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5

Ginger Extraction and Characterization

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Ginger was obtained from a local Slovenian vendor (Mercator d.d., Ljubljana, Slovenia). The natural material was dried by a lyophilization process (Tlyophilization= −30 °C, tlyophilization= 72 h, p = 0.04 mbar vacuum) and then ground using a grinder before extraction procedures. The mean particle size has been determined by sieve analysis and was 0.5 mm.
Chemicals used in this study were ethanol (C2H6O) (Sigma-Aldrich, St. Louis, MO, USA, HPLC grade, ≥99.9%), carbon dioxide (CO2) (Messer; MG, Ruše, Slovenia, purity 2.5), 2,2-diphenyl-1-picrylhydrazyl (DPPH) (SigmaAldrich, Darmstadt, Germany, ≥97.0%), Folin-Ciocalten reagent (FC) (Sigma Aldrich), sodium(V)carbonate (Na2CO3) (Sigma Aldrich, ≥99.9%), gallic acid (C7H6O5) (Sigma Aldrich, 97.5–102.5%), and methanol (H3OH) (Honeywell, Charlotte, NC, USA, LC-MS CHROMASOLV®, ≥99.9%).
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6

Comprehensive Chemical Synthesis Protocol

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Urea (CH4N2O, Proquimsa, Coahuila, Mexico), phosphorus pentoxide (P2O5, Sigma-Aldrich, St. Louis, MO, USA), zinc powder (Chemical Dynamics, Florida, USA), ethanol (C2H6O, Sigma-Aldrich, St. Louis, MO, USA), methanol (CH4O, Sigma-Aldrich, St. Louis, MO, USA), acetone (C3H6O, Sigma-Aldrich, St. Louis, MO, USA), Toluene (C7H8, Sigma-Aldrich, St. Louis, MO, USA), Hexane (Sigma-Aldrich, St. Louis, MO, USA), Phosphoric Acid (H3PO4, Fermont, Nuevo León, Mexico), Ammonium Hydroxide 30% (NH4OH, Fermont, Nuevo León, Mexico), Hydrochloric Acid 30% (HCl, Fermont, Nuevo León, Mexico), porcine hematin (Sigma-Aldrich, St. Louis, MO, USA), aniline (Sigma-Aldrich, St. Louis, MO, USA), sodium carbonate (Na2CO3, Sigma-Aldrich, St. Louis, MO, USA), sodium bicarbonate (NaHCO3, Sigma-Aldrich St. Louis, MO, USA), p-toluene sulfonic acid (TSA, Sigma-Aldrich, St. Louis, MO, USA), hydrogen peroxide (H2O2, Sigma-Aldrich, St. Louis, MO, USA), Dimethyl sulfoxide (DMSO, Sigma-Aldrich, St. Louis, MO, USA), N-Methyl-2-pyrrolidone (NMP, Sigma-Aldrich, St. Louis, MO, USA), deuterated sulfuric acid (D2SO4, Sigma-Aldrich, St. Louis, MO, USA), deuterated DMSO (Sigma-Aldrich, St. Louis, MO, USA), deuterated acetone (Sigma-Aldrich, St. Louis, MO, USA) and deuterated water (D2O, Sigma-Aldrich, St. Louis, MO, USA).
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7

Quantitative Diclofenac Determination Assay

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Ammonium heptamolybdate ((NH4)6Mo7O24), hydrochloric acid (HCl 37%), sodium hydroxide (NaOH), ethanol (C2H6O) and diclofenac sodium (C14H10Cl2NNaO2) were purchased from Sigma Aldrich (St. Louis, MO, USA), Urea (CO(NH2)2) was bought from Merck (Darmstadt, Germany). All chemicals used were of high purity (i.e., 98–99%).
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8

Fabrication of ZnO Nanorods on Glass

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To fabricate the ZnO NRs on glass substrates (25 mm × 75 mm), analytical reagents such as zinc nitrate hexahydrate [Zn (NO3)2·6H2O; purity >99.8%] was obtained from Sigma Aldrich. Zinc acetate dihydrate (Zn(CH3OO)2·2H2O; purity >99% Merck) and hexamethylenetetramine (C6H12N4; purity >99% Merck) were purchased from Billerica, USA. The docosane (n-docosane, purity >99% Sigma Aldrich) was supplied by Supelco, Bellefonte, USA, and ethanol (C2H6O, purity >99%, Sigma Aldrich) and deionized water were used to prepare all aqueous solutions.24 Throughout the experiment, organic solvents with purity levels greater than analytical grade were utilised.
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9

Extraction and Characterization of Basil Leaves

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All chemicals were used as-received, and deionized water (D.H2O, 18.2 µΩ cm−1) was used to prepare all solutions / suspension. All phenol (99.0%), p-Nitrophenol (99%), and p-Methoxyphenol (99.0%), were purchased from Sigma Aldrich. Tin(IV) Chloride (SnCl4.5H2O, 99%) was purchased from Gainland Chemical Company. Ethanol (C2H6O, 99.5%) was obtained from Merck. The fresh leaves of O. basilicum were collected from the Al-Karak area, Jordan, and taxonomically identified by the Department of Biological Sciences, Faculty of Science, Mu'tah University, Al-Karak, Jordan.
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10

Polyamide 6 Polymer Characterization

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Polyamide 6 polymer, with the linear formula of [–NH(CH2)5CO–]n, used in this work, was purchased from Sigma-Aldrich with a density of 1.084 g/mL at the temperature of 25 °C and the glass transition temperature of 47 °C. DR1 dye, formic acid (HCOOH), and ethanol (C2H6O) as solvents and dimethylformamide (DMF) were obtained from Merck. The water used in this work was distilled water.
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