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Brdu bu20a pe

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BrdU (BU20A) PE is a fluorescently labeled antibody used to detect the incorporation of the synthetic nucleoside bromodeoxyuridine (BrdU) into cellular DNA. It can be used to measure cell proliferation and DNA synthesis.

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Lab products found in correlation

Brdu flow kit, by BD (2 mentions) Imject alum adjuvant, by Thermo Fisher Scientific (2 mentions) Np20 cgg, by Biosearch Technologies (2 mentions) Igm 2 41 apc, by Thermo Fisher Scientific (2 mentions) Igg1 a85 1 pe, by BD (2 mentions)

2 protocols using brdu bu20a pe

1

Immunization and BrdU Incorporation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mice were immunized i.p. or i.v. with 100μl of PBS-washed citrated SRBCs (Colorado Serum Company) or i.p. with 50μg NP20-CGG (Biosearch Technologies) plus Imject Alum Adjuvant (ThermoFisher Scientific) in 100μl PBS. For SRBC-specific antibody titers, serum was collected on indicated days and bound to SRBCs and detected with IgM (II/41) APC (Thermo Fisher Scientific) and IgG1 (A85–1) PE (BD Biosciences) by flow cytometry. For BrdU pulse experiments, mice were injected i.p. with 2mg BrdU in 200μl PBS and sacrificed 2 h later. Incorporation of BrdU was detected using the BrdU Flow Kit (BD Biosciences) according to manufacturer’s instructions and BrdU (BU20A) PE (Thermo Fisher Scientific).
Ramezani-Rad P., Chen C., Zhu Z, & Rickert R.C. (2020). Cyclin D3 Governs Clonal Expansion of Dark Zone Germinal Center B Cells. Cell reports, 33(7), 108403.
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2

Immunization and BrdU Incorporation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mice were immunized i.p. or i.v. with 100μl of PBS-washed citrated SRBCs (Colorado Serum Company) or i.p. with 50μg NP20-CGG (Biosearch Technologies) plus Imject Alum Adjuvant (ThermoFisher Scientific) in 100μl PBS. For SRBC-specific antibody titers, serum was collected on indicated days and bound to SRBCs and detected with IgM (II/41) APC (Thermo Fisher Scientific) and IgG1 (A85–1) PE (BD Biosciences) by flow cytometry. For BrdU pulse experiments, mice were injected i.p. with 2mg BrdU in 200μl PBS and sacrificed 2 h later. Incorporation of BrdU was detected using the BrdU Flow Kit (BD Biosciences) according to manufacturer’s instructions and BrdU (BU20A) PE (Thermo Fisher Scientific).
Ramezani-Rad P., Chen C., Zhu Z, & Rickert R.C. (2020). Cyclin D3 Governs Clonal Expansion of Dark Zone Germinal Center B Cells. Cell reports, 33(7), 108403.
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