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Fmoc lys boc wang resin

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Fmoc-Lys(Boc)-Wang Resin is a solid-phase peptide synthesis (SPPS) resin used for the production of peptides and proteins. It consists of a Wang resin matrix with the Fmoc-protected amino acid Fmoc-Lys(Boc) pre-loaded onto it. This resin is commonly used in the Fmoc-SPPS method to introduce the first lysine residue into the growing peptide chain.

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Lab products found in correlation

Q3 system, by Merck Group (3 mentions) Phenol, by Merck Group (3 mentions) Trifluoroacetic acid, by Merck Group (3 mentions) Methyl tert butyl ether (mtbe), by Merck Group (3 mentions) Dichloromethane, by Merck Group (3 mentions) Dimethylformamide, by Merck Group (3 mentions) N n diisopropylethylamine, by Merck Group (3 mentions) Acetic anhydride, by Merck Group (3 mentions) N n diisopropylcarbodiimide, by Merck Group (3 mentions) Hydroxybenzotriazole, by Merck Group (3 mentions) O 7 azabenzotriazol 1 yl n n n n tetramethyluronium hexafluorophosphate hatu, by Merck Group (3 mentions) Acetic acid, by Merck Group (3 mentions) Thioanisol, by Merck Group (3 mentions) N trityl 1 2 ethanediamine, by Merck Group (3 mentions) Lysotracker, by Thermo Fisher Scientific (2 mentions) Iodine, by Merck Group (2 mentions) Bacmam 2, by Thermo Fisher Scientific (2 mentions) Bodipy tr ceramide, by Thermo Fisher Scientific (2 mentions) Wang resin fmoc tyr tbu wang resin, by AAPPTec (2 mentions) Celllight peroxisome gfp, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

Wang resin (4 citations)

5 protocols using fmoc lys boc wang resin

1

Fluorenylmethyloxy carbonyl peptide synthesis

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The fluorenylmethyloxy carbonyl(Fmoc) amino acids and the Fmoc-Lys(Boc)-Wang Resin were purchased from AAPPTec (Louisville, KY, USA). All the other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA) and used without further purification. Chlorin e6 (Ce6) was purchased from Frontier-Scientific (Logan, UT, USA). Water was obtained from Millipore Q3 system (Billerica, MA, USA).
Dulbecco’s modified Eagle’s medium (DMEM) culture medium, fetal calf serum, and penicillin–streptomycin were purchased from Gibco (Life Technologies, NY, USA). Lysotracker green, mitotracker green, ER tracker green, HPF, Amplex Red assay, and Calcein AM were purchased from Thermo Fisher Scientific.
Gilson R.C., Tang R., Gautam K.S., Grabowska D, & Achilefu S. (2019). Trafficking of a Single Photosensitizing Molecule to Different Intracellular Organelles Demonstrates Effective Hydroxyl Radical-Mediated Photodynamic Therapy in the Endoplasmic Reticulum. Bioconjugate chemistry, 30(5), 1451-1458.
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2

Peptide Synthesis and Immunofluorescence Assay

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All the fluorenylmethyloxycarbonyl (Fmoc) amino acids and Fmoc-Lys (Boc)-Wang Resin were purchased from AAPPTec (Louisville, KY, USA). Dichloromethane (DCM), acetic acid, acetic anhydride, thioanisole, phenol, hydroxybenzotriazole (HOBt), N,N-diisopropylethylamine (DIEA), N-trityl-1,2-ethanediamine, phenol, thioanisol, dimethylformamide (DMF), N,N′-diisopropylcarbodiimide (DIC), trifluoroacetic acid (TFA), iodine, methyl tert-butyl ether (MTBE), O-(7-azabenzotriazol-1-yl)-N,N,N′,N′-tetramethyluronium hexafluorophosphate (HATU), and dexamethasone (DEX) were purchased from Sigma-Aldrich (St Louis, MO). Hematoxylin and eosin (H&E) stains were purchased from MilliporeSigma (St Louis, MO). Rabbit anti-pANXA2 (phospho-Tyr24) antibody was purchased from Signalway Antibody (College Park, MD). AlexaFluor 594-conjugated donkey anti-rabbit antibody was purchased from Thermo Fisher Scientific (Waltham, MA).
Tsen S.W., Springer L.E., Sharmah Gautam K., Tang R., Liang K., Sudlow G., Kucharski A., Pham C.T, & Achilefu S. (2021). Non-invasive monitoring of arthritis treatment response via targeting of tyrosine-phosphorylated annexin A2 in chondrocytes. Arthritis Research & Therapy, 23, 265.
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3

Peptide Synthesis and Plasmid DNA Purification

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Fmoc-amino acids, Fmoc-Lys (Boc)-Wang Resin, N-hydroxybenzotriazole (HOBt), and 1-[Bis(dimethylamino)methylene]-1H-1,2,3-triazolo[4,5-b]pyridinium 3-oxide hexafluorophosphate, N-[(Dimethylamino)-1H-1,2,3-triazolo-[4,5-b]pyridin-1-ylmethylene]-N-methylmethanaminium hexafluorophosphate N-oxide (HATU) were from AAPPTec (Louisville, KY, U.S.A.). Trifluoroacetic acid (TFA), acetonitrile, diisoproplyethylamine (DIPEA), 1,2-Ethane dithiol and piperidine were purchased from Sigma Chemical Co. (St Louis, MO, U.S.A.). ACS grade N,N dimethylformamide (DMF) and Dichloromethane (DCM) were supplied by BDH Chemicals ( PA, USA). Maleimide-mPEG (5kD) was from Laysan Bio (Arab, AL, U.S.A.). pGL3 control vector, a 5.3-kbp luciferase plasmid containing a SV40 promoter and enhancer, was obtained from Promega (Madison, WI, U.S.A.). Trypsin was from Worthington (Lakewood, NJ). Tris (1 M pH 8.0) phenol/chloroform/isoamyl alcohol was purchased from Fisher Scientific (Pittsburgh, PA, U.S.A.). Lithium borate electrophoresis buffer was from Faster Better Media (Hunt Valley, MD, USA).
Mathew B., Ramanathan R., Delvaux N., Poliskey J, & Rice K.G. (2020). Heat Shrinking DNA Nanoparticles for In Vivo Gene Delivery. Gene therapy, 27(5), 196-208.
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4

Peptide Synthesis and Cellular Assays

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All the fluorenylmethyloxycarbonyl (Fmoc) amino acids, Wang resin Fmoc-Tyr(tBu)-Wang resin and Fmoc-Lys(Boc)-Wang Resin were purchased from AAPPTec (Louisville, KY, USA). Dichloromethane (DCM), acetic acid, acetic anhydride, phenol, hydroxybenzotriazole (HOBt), N,N-diisopropylethylamine (DIEA), N-trityl-1,2-ethanediamine, phenol, thioanisol, dimethylformamide (DMF), N,N’-diisopropylcarbodiimide (DIC), trifluoroacetic acid (TFA), iodine, methyl tert-butyl ether (MTBE) and O-(7-azabenzotriazol-1-yl)-N,N,N’,N’-tetramethyluronium hexafluorophosphate (HATU) were purchased from Sigma-Aldrich (St. Louis, MO, USA). For a full list of chemicals used and their details, please refer to Supplementary Table 1. Water was obtained from a Millipore Q3 system. Lysotracker, BODIPY TR Ceramide, CellLight Peroxisome-GFP, BacMam 2.0, and Fluo-4 calcium probe were purchased from Thermo Fisher Scientific (Waltham, MA). Chlorpromazine hydrochloride, filipin complex, and amiloride hydrochloride were purchased from Sigma-Aldrich (St Louis, MO, USA). Reagents for organelle staining included VectaCell Rhodamine 123 (Vector Laboratories, Burlingame, CA, USA).
Shen D., Xu B., Liang K., Tang R., Sudlow G.P., Egbulefu C., Guo K., Som A., Gilson R., Maji D., Mondal S., Habimana-Griffin L., Akers W., Li S., Liu Y., Bloch S., Kurkure S., Nussinov Z., Seidel A., Tsen S.W, & Achilefu S. (2020). Selective imaging of solid tumours via the calcium-dependent high-affinity binding of a cyclic octapeptide to phosphorylated Annexin A2. Nature biomedical engineering, 4(3), 298-313.
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5

Peptide Synthesis and Cellular Assays

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All the fluorenylmethyloxycarbonyl (Fmoc) amino acids, Wang resin Fmoc-Tyr(tBu)-Wang resin and Fmoc-Lys(Boc)-Wang Resin were purchased from AAPPTec (Louisville, KY, USA). Dichloromethane (DCM), acetic acid, acetic anhydride, phenol, hydroxybenzotriazole (HOBt), N,N-diisopropylethylamine (DIEA), N-trityl-1,2-ethanediamine, phenol, thioanisol, dimethylformamide (DMF), N,N’-diisopropylcarbodiimide (DIC), trifluoroacetic acid (TFA), iodine, methyl tert-butyl ether (MTBE) and O-(7-azabenzotriazol-1-yl)-N,N,N’,N’-tetramethyluronium hexafluorophosphate (HATU) were purchased from Sigma-Aldrich (St. Louis, MO, USA). For a full list of chemicals used and their details, please refer to Supplementary Table 1. Water was obtained from a Millipore Q3 system. Lysotracker, BODIPY TR Ceramide, CellLight Peroxisome-GFP, BacMam 2.0, and Fluo-4 calcium probe were purchased from Thermo Fisher Scientific (Waltham, MA). Chlorpromazine hydrochloride, filipin complex, and amiloride hydrochloride were purchased from Sigma-Aldrich (St Louis, MO, USA). Reagents for organelle staining included VectaCell Rhodamine 123 (Vector Laboratories, Burlingame, CA, USA).
Shen D., Xu B., Liang K., Tang R., Sudlow G.P., Egbulefu C., Guo K., Som A., Gilson R., Maji D., Mondal S., Habimana-Griffin L., Akers W., Li S., Liu Y., Bloch S., Kurkure S., Nussinov Z., Seidel A., Tsen S.W, & Achilefu S. (2020). Selective imaging of solid tumours via the calcium-dependent high-affinity binding of a cyclic octapeptide to phosphorylated Annexin A2. Nature biomedical engineering, 4(3), 298-313.
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