4T1 murine breast tumor cells were seeded in 6-well plates with sterile coverslips (2.5 × 105 cells/well) at 24 h before treatment. Cells were exposed to 3μM of DOX, SpHL-DOX, and SpHL-DOX-Fol for 24 h. After, the treatments were withdrawn and wells were washed with PBS buffer. Cells were fixed with 3.7% (v/v) formaldehyde and permeabilized with 0.1% (v/v) Triton X-100 solution [26 (link)]. Cell membranes and nuclei were labeled with fluorescent probes Cholera toxin B subunit-Alexa Fluor® 647 (Invitrogen - Carlsbad, USA) and Hoechst 33258 (Thermo Fisher Scientific - Waltham, USA), respectively. The coverslips were washed with PBS and slides were assembled using Prolong Gold Antifade Reagent (Thermo Fisher Scientific - Waltham, USA). Cells were analyzed in “Centro de Aquisição e Processamento de Imagens da UFMG (CAPI/UFMG)” using the LSM 880 microscope with Airyscan detector (ZEISS - Oberkochen, Germany). For image acquisition, it was used 40x objective lens. The lasers used were: Diode 405 nm (excitation of Hoechst 33258), Argonium 488 nm (excitation of DOX) and HeNe 633 nm (excitation of Alexa Fluor®647). The images were processed using the ZEN Blue Edition software version 2.3 lite (ZEISS - Oberkochen, Germany).
Zen blue edition software version 2.3 lite
Manufactured by Zeiss
Sourced in Germany
ZEN Blue Edition software version 2.3 lite is a basic imaging and analysis software developed by Zeiss. It provides essential functionalities for image acquisition, processing, and visualization of microscopy data.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using zen blue edition software version 2.3 lite
1
Fluorescence Imaging of 4T1 Cells
2
Fluorescence Imaging of 4T1 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
4T1 murine breast tumor cells were seeded in 6-well plates with sterile coverslips (2.5 × 105 cells/well) at 24 h before treatment. Cells were exposed to 3μM of DOX, SpHL-DOX, and SpHL-DOX-Fol for 24 h. After, the treatments were withdrawn and wells were washed with PBS buffer. Cells were fixed with 3.7% (v/v) formaldehyde and permeabilized with 0.1% (v/v) Triton X-100 solution [26 (link)]. Cell membranes and nuclei were labeled with fluorescent probes Cholera toxin B subunit-Alexa Fluor® 647 (Invitrogen - Carlsbad, USA) and Hoechst 33258 (Thermo Fisher Scientific - Waltham, USA), respectively. The coverslips were washed with PBS and slides were assembled using Prolong Gold Antifade Reagent (Thermo Fisher Scientific - Waltham, USA). Cells were analyzed in “Centro de Aquisição e Processamento de Imagens da UFMG (CAPI/UFMG)” using the LSM 880 microscope with Airyscan detector (ZEISS - Oberkochen, Germany). For image acquisition, it was used 40x objective lens. The lasers used were: Diode 405 nm (excitation of Hoechst 33258), Argonium 488 nm (excitation of DOX) and HeNe 633 nm (excitation of Alexa Fluor®647). The images were processed using the ZEN Blue Edition software version 2.3 lite (ZEISS - Oberkochen, Germany).
3
Cellular Internalization of Novel Irinotecan Formulations
4
Cellular Internalization of Novel Irinotecan Formulations
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CT26 murine colon cancer tumor cells were seeded in 6-well plates with sterile coverslips (2.5 × 105 cells/well) at 24 h before treatment. Cells were exposed to a solution of IRN, SpHL-IRN, or SpHL-IRN-Fol, at a drug concentration of 5 μM, for 12 h. After washing with PBS, cells were fixed with 3.7% (v/v) formaldehyde solution, slides were assembled using Prolong Gold Antifade Reagent (Thermo Fisher Scientific - Waltham, USA). Cells were analyzed in “Centro de Aquisição e Processamento de Imagens da UFMG (CAPI/UFMG)” using the LSM 880 microscope with Airyscan detector (ZEISS - Oberkochen, Germany). For image acquisition, a 63x objective lens and a Diode 370 nm (excitation of IRN) laser were used. The images were processed using the ZEN Blue Edition software version 2.3 lite (ZEISS -Oberkochen, Germany).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!