Ventana autostaining platform

Manufactured by Roche

The Ventana autostaining platform is a laboratory equipment product designed to automate the immunohistochemistry (IHC) and in situ hybridization (ISH) staining process. It is used to prepare and stain tissue samples for analysis in pathology and diagnostic laboratories.

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Lab products found in correlation

Optiview dab ihc detection kit, by Roche (1 mentions) Ventana discovery, by Roche (1 mentions)

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Ventana platform (12 citations)

2 protocols using ventana autostaining platform

Comprehensive Immunohistochemistry Profiling

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Immunohistochemistry for CD8 (Cell Marque 760-4250), PD-1 (Cell Marque 315M-96), CD163 (Novacastra NCL-CD163), MLH-1 (Ventana 790-5091), PMS2 (Ventana 790-5094), MSH2 (Ventana 790-5093), and MSH6 (Ventana 790-5092) were performed according to previous validated protocols in the Johns Hopkins CLIA-certified laboratory using the Ventana autostaining platform (Roche). Immunohistochemistry for FoxP3 (Abcam 20034, dilution 1:100) was performed with the OptiView DAB IHC Detection Kit (Roche) on a Ventana Discovery autostaining platform (Roche). Immunohistochemistry for NF-κB p65 Rabbit mAb (Cell Signaling 8242T, dilution 1:400) was performed with the OptiView DAB IHC Detection Kit (Roche) on a Ventana Discovery autostaining platform (Roche). A paraffin-embedded LNCaP cell line tissue microarray was used to validate the p65 immunostain. The immunohistochemistry stains were evaluated by two trained pathologists (D.C.S. and T.L.L.).

Sena L.A., Salles D.C., Engle E.L., Zhu Q., Tukachinsky H., Lotan T.L, & Antonarakis E.S. (2021). Mismatch repair–deficient prostate cancer with parenchymal brain metastases treated with immune checkpoint blockade. Cold Spring Harbor Molecular Case Studies, 7(4), a006094.

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Immunohistochemistry for MMR Proteins

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IHC for four MMR proteins (MLH1, PMS2, MSH2 and MSH6) was performed on formalin‐fixed, paraffin‐embedded tissue obtained from representative sections of the resection specimens. MLH-1 (Ventana 790–5091), PMS2 (Ventana 790–5094), MSH2 (Ventana 790–5093), and MSH6 (Ventana 790–5092) were performed according to previously validated protocols using the Ventana auto-staining platform (Roche). Normal colonic crypt epithelium adjacent to the tumor, lymphoid cells and stromal cells served as internal positive controls. The cases with loss of any of MMR protein expression were defined as dMMR, and the cases without loss of MMR protein expression were defined as pMMR (Supplement Fig).

Wang Y., Zheng R., Zhang Y., Guo Y., Hui Z., Wang P, & Sun Y. (2022). Galectin-9 expression clinically associated with mature dendritic cells infiltration and T cell immune response in colorectal cancer. BMC Cancer, 22, 1319.

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