HBV core and PF DNAs were extracted as previously described and analyzed by Southern blotting [11 (link)]. Whole-cell extracts from a portion of transfected cells were fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and analyzed by western blotting for Tdp2 using polyclonal rabbit anti-human Tdp2 [20 (link)] or a commercial anti-Tdp2 antibody (Bethyl Labs) and HBV core protein using a mouse monoclonal antibody specific for the N-terminal end of core protein [41 (link)] respectively. To control for loading, western blotting for flap endonuclease 1 (FEN-1) or β- actin was carried out using monoclonal mouse anti-FEN-1 (BD Transduction Laboratories) or β- actin (Cell Signaling Technology). Southern and western blotting data were quantified by phosphorimaging and densitometry.
Anti tdp2 antibody
Manufactured by Fortis Life Sciences
The Anti-TDP2 antibody is a laboratory research tool that can be used to detect and quantify the presence of the TDP2 protein in biological samples. TDP2 is an enzyme involved in DNA repair processes. The antibody can be utilized in various analytical techniques, such as Western blotting, immunohistochemistry, and ELISA, to study the expression and localization of TDP2 in different cell types and tissues.
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Lab products found in correlation
3 protocols using anti tdp2 antibody
1
HBV DNA and Protein Analysis
2
Plasmid Construction and Mutant Cell Isolation
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The following oligonucleotides (IDT, IA) were used for constructing plasmids that express PolQ or TDP2 gRNAs (PAM sequences are in parenthesis and not part of oligos): TTCATATAGGAGTTCATCA(TGG)/TGATGAACTCCTATATGAA(PolQ); ATATAACTGTAGCT GACTC(TGG)/GAGTCAGCTACAGTTATAT (TDP2).
Mutant cells were isolated by the HPRT co-targeting method as previously described (Liao et al., 2015 (link)). PolQ status was determined by amplifying the target region from wild-type and mutant cells and then Sanger sequencing (Genewiz, NJ). TPD2 status was determined by western blot with an anti-TDP2 antibody (Bethyl Laboratories, TX) against samples prepared from wild-type and mutant cells. Primary murine bone marrow cells were isolated from Polq+/+ and Polq−/− mice, which were obtained by breeding Polq+/− mice (JAX #006194). Lin-cKIT+ primary cells were isolated by magnetic sorting using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (StemCell) followed by EasySep Mouse CD117 (cKIT) Positive Selection Kit (StemCell), and were subsequently cultured in IMDM + 10% FBS supplemented with a cocktail of growth factors (3 ng/mL IL3, 3 ng/mL IL6, 5 ng/mL SCF). Polq+/+ and Polq−/− mESCs and iPSCs were generated in prior studies as described (Mateos-Gomez et al., 2015 (link), 2017 (link)).
Mutant cells were isolated by the HPRT co-targeting method as previously described (Liao et al., 2015 (link)). PolQ status was determined by amplifying the target region from wild-type and mutant cells and then Sanger sequencing (Genewiz, NJ). TPD2 status was determined by western blot with an anti-TDP2 antibody (Bethyl Laboratories, TX) against samples prepared from wild-type and mutant cells. Primary murine bone marrow cells were isolated from Polq+/+ and Polq−/− mice, which were obtained by breeding Polq+/− mice (JAX #006194). Lin-cKIT+ primary cells were isolated by magnetic sorting using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (StemCell) followed by EasySep Mouse CD117 (cKIT) Positive Selection Kit (StemCell), and were subsequently cultured in IMDM + 10% FBS supplemented with a cocktail of growth factors (3 ng/mL IL3, 3 ng/mL IL6, 5 ng/mL SCF). Polq+/+ and Polq−/− mESCs and iPSCs were generated in prior studies as described (Mateos-Gomez et al., 2015 (link), 2017 (link)).
3
Plasmid Construction and Mutant Cell Isolation
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