Clarus 500 chromatograph

Analyses were performed on a Clarus 500 PerkinElmer Chromatograph (PerkinElmer, Courtaboeuf, France), equipped with flame ionization detector (FID) and two fused-silica capillary columns (50 m × 0.22 mm, film thickness 0.25 µm), BP-1 (polydimethylsiloxane), and BP-20 (polyethylene glycol). The oven temperature was programmed from 60 °C to 220 °C at 2 °C/min and then held isothermal at 220 °C for 20 min; injector temperature: 250 °C; detector temperature: 250 °C; carrier gas: hydrogen (0.8 mL/min); split: 1/60; injected volume: 0.5 µL. Retention indices (RI) were calculated relative to the retention times of a series of n-alkanes (C8–C29) with linear interpolation (“Target Compounds” software from PerkinElmer, Courtaboeuf, France). The quantification of volatile compounds was obtained using Relative Response Factor (RFF), calculated according to the International Organization of the Flavor Industry (IOFI) [32 (link)]. The relative proportion of each compound (expressed in g/100 g) was calculated using the amount of EO and reference (Methyl octanoate), peak area and relative response factors.

Analyses were performed on a Clarus 500 PerkinElmer Chromatograph (PerkinElmer, Courtaboeuf, France), equipped with a flame ionization detector (FID) and two fused-silica capillary columns (50 m × 0.22 mm, film thickness 0.25 µm), BP-1 (polydimethylsiloxane) and BP-20 (polyethylene glycol). The oven temperature was programmed from 60 °C to 220 °C at 2 °C/min and then held isothermal at 220 °C for 20 min; injector temperature: 250 °C; detector temperature: 250 °C; carrier gas: hydrogen (0.8 mL/min); split: 1/60; injected volume: 0.5 µL. Retention indices (RI) were determined relative to the retention times of a series of n-alkanes (C8–C29) with a linear interpolation (« Target Compounds » software from PerkinElmer). The relative response factor (RFF) of each compound was calculated according to the International Organization of the Flavor Industry (IOFI)-recommended practice for the use of predicted relative response factors for the rapid quantification of volatile flavoring compounds by GC(FID) [36 (link)]. Methyl octanoate was used as an internal reference, and the relative proportion of each constituent (expressed in g/100 g) was calculated using the weight of the essential oil and reference, peak area and relative response factors (RRF).

Analyses were performed on a Clarus 500 PerkinElmer Chromatograph (PerkinElmer, Courtaboeuf, France), equipped with flame ionization detector (FID) and two fused-silica capillary columns (50 m × 0.22 mm, film thickness 0.25 µm), BP-1 (polydimethylsiloxane) and BP-20 (polyethylene glycol). The oven temperature was programmed from 60 °C to 220 °C at 2 °C/min and then held isothermal at 220 °C for 20 min; injector temperature: 250 °C; detector temperature: 250 °C; carrier gas: helium (0.8 mL/min); split: 1/60; injected volume: 0.5 µL. Retention indices (RI) were determined relative to the retention times of a series of n-alkanes (C8–C29) with linear interpolation (« Target Compounds » software from PerkinElmer). The relative response factor (RFF) of each compound was calculated according to the IOFI recommended practice for the use of predicted relative response factors for the rapid quantification of volatile flavoring compounds by GC(FID) [23 (link)]. Methyl octanoate was used as an internal reference and the relative proportion of each constituent (expressed in g/100 g) was calculated using the weight of essential oil and reference, peak area and relative response factors (RRF).