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Truseq dna preparation kit

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The TruSeq DNA Preparation Kit is a laboratory equipment product designed for the preparation of DNA samples for sequencing. The kit provides the necessary reagents and protocols to enable the construction of DNA libraries from a variety of input DNA sources.

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Lab products found in correlation

Hiseq 2000, by Illumina (3 mentions) Hiseq 2000 sequencing platform, by Illumina (2 mentions) Hiseq platform, by Illumina (1 mentions) Hiseq 2500 platform, by Illumina (1 mentions) Genomic tip 100 g, by Qiagen (1 mentions) Truseq exome enrichment kit, by Illumina (1 mentions) Paired end library preparation kit, by Illumina (1 mentions) Agilent bioanalyzer, by Agilent Technologies (1 mentions) 480 2 light cycler, by Roche (1 mentions) Casava software suite, by Illumina (1 mentions)

Spelling variants of this product

TruSeq kits (44 citations) TruSeq PCR-free DNA sample preparation kits (4 citations) TruSeq DNA Methylation library preparation kits (2 citations) TruSeq DNA Sample Preparation Kits V2 (2 citations)

6 protocols using truseq dna preparation kit

1

Whole-Genome Resequencing of Brassica napus

Cited 1 time
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Libraries from high‐quality genomic DNA from both parental lines, BC1329, and BC9102, were constructed using the Illumina TruSeq DNA Preparation Kit, following the manufacturer's instructions (Illumina). Whole‐genome resequencing (2 × 150 bp) was performed at the Novogene facility (Novogene Co., Ltd.) using the Illumina HiSeq 2000 sequencing platform. The coverage of the parental lines ranged from 77.6 (BC1329, 102.6 Gb) to 83.8× (BC 9102, 112.4 Gb). Read mapping to the “Darmor‐bzh” reference assembly (version 4.1, http://www.genoscope.cns.fr/brassicanapus/data/), single nucleotide polymorphisms (SNP) and InDel (<50 bp) calling, structural variation (SV, ≥50 bp) detection, and identification of HE event (≥10‐kb windows) were performed as described in Raman et al. (2021 (link)). Details of HE analysis are described in Supporting Information: Method S1.
Raman H., Raman R., Pirathiban R., McVittie B., Sharma N., Liu S., Qiu Y., Zhu A., Kilian A., Cullis B., Farquhar G.D., Stuart‐Williams H., White R., Tabah D., Easton A, & Zhang Y. (2022). Multienvironment QTL analysis delineates a major locus associated with homoeologous exchanges for water‐use efficiency and seed yield in canola. Plant, Cell & Environment, 45(7), 2019-2036.
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2

Whole-Genome Sequencing of P. cuspidatum

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P. cuspidatum plants were cultivated in a field in Hubei Province, China (25°20ʹ5.496ʹʹ N, 114?57ʹ52.459ʹʹE). The fresh leaves of 1-year-old plants were collected for DNA extraction. To construct sequencing libraries, 5 μg of DNA was used. Libraries were constructed using an Illumina TruSeq DNA Preparation Kit following the manufacturer’s recommendations. The four sequencing libraries, with insert sizes of 550 bp, 2 to 3 kb, 5 to 7 kb, and 10 to 15 kb, were sequenced on the Illumina HiSeq platform (150-bp paired-end reads, PE150). In addition, 250-bp paired-end reads were generated on the Illumina HiSeq 2500 platform.
Zhang Y., Zheng L., Zheng Y., Zhou C., Huang P., Xiao X., Zhao Y., Hao X., Hu Z., Chen Q., Li H., Wang X., Fukushima K., Wang G, & Li C. (2019). Assembly and Annotation of a Draft Genome of the Medicinal Plant Polygonum cuspidatum. Frontiers in Plant Science, 10, 1274.
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3

Genome Sequencing of Vibrio campbellii 1114GL

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The V. campbellii 1114GL strain, provided by Timothy William Flegel, Centex Shrimp, Faculty of Science, Mahidol University, Bangkok, Thailand, was named VH1114GL in a previous study28 (link). It was cultured from a glycerol stock in MHB + 3% ASW at 30 °C with shaking at 200 rpm for 16 hours. The bacterial cells were pelleted by centrifugation at 5,000 × g for 10 min. Genomic DNA was extracted using Qiagen Genomic-tip 100/G according to the manufacturer’s instructions. For genome sequencing, Illumina and Roche 454 platforms were used. Two paired-end libraries (insert size = ~320 bp) were constructed using the TruSeq DNA Preparation Kit with the standard protocol (Illumina) and sequenced by Illumina MiSeq to produce 250-bp paired end reads. Three mate-pair libraries of various jumping sizes (2 kb, 4 kb, and 6 kb) were constructed using the Nextera Mate Pair Sample Preparation Kit and sequenced by Illumina HiSeq2000 to produce 100-bp mate pair reads. The long single-end reads were produced by 454 GS FLX+.
Ke H.M., Prachumwat A., Yu C.P., Yang Y.T., Promsri S., Liu K.F., Lo C.F., Lu M.Y., Lai M.C., Tsai I.J, & Li W.H. (2017). Comparative genomics of Vibrio campbellii strains and core species of the Vibrio Harveyi clade. Scientific Reports, 7, 41394.
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4

Whole Exome Sequencing of sIBM Patients

Cited 3 times
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Indexed genomic DNA libraries were prepared from genomic DNA using TruSeq DNA Preparation Kit (Illumina, San Diego, CA, USA) and exome capture using TruSeq Exome Enrichment Kit (Illumina), according to the manufacturer’s protocol. Sequencing was performed with 100 bp paired-end reads on a HiSeq2000 (Illumina). Reads were aligned to the human reference genome with NovoAlign (Novocraft Technologies, Selangor, Malaysia) or Burrows-Wheeler Aligner.18 (link) Variants were called with SAMtools19 (link) and annotated with SeattleSeq. Coverage across genomic intervals was calculated using BEDTools.20 (link) Genomic coordinates for regions targeted by the whole-exome capture kit were provided by Illumina. Whole exome sequences from 62 sIBM patients were filtered for variants that: 1) had a minor allele frequency of ≤0.001 in the ExAC Database; 2) generated a loss of function variant or a nonsynonymous change; and 3) fulfilled a strict sequence quality as defined by Genesis 2.0 software.
Güttsches A.K., Brady S., Krause K., Maerkens A., Uszkoreit J., Eisenacher M., Schreiner A., Galozzi S., Mertens-Rill J., Tegenthoff M., Holton J.L., Harms M.B., Lloyd T.E., Vorgerd M., Weihl C.C., Marcus K, & Kley R.A. (2017). Proteomics of rimmed vacuoles define new risk allele in inclusion body myositis. Annals of neurology, 81(2), 227-239.
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5

Paired-End Illumina DNA Library Preparation

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Two paired-end libraries were prepared from 1.1 μg of gDNA using Illumina TruSeq DNA Preparation Kit, following Illumina’s standard protocol (Paired-end Library Preparation Kit, Illumina, SanDiego, CA, USA). Shearing of gDNA was done using Covaris S series (Covaris, MS, USA). Following end repair, A-tailing, and adaptor ligation, DNA in the 500–600 bp range was purified from a 2% agarose gel. DNA was then PCR enriched for a total of ten cycles. Proper DNA size was then confirmed with the Agilent Bioanalyzer, followed by qPCR quantification with Roche Light Cycler 480 II and Kapa Biosystems reagents.
Cluster generation was performed on an Illumina cBot and the libraries were sequenced on an Illumina HiSeq 2000 following the Paired-End protocol. Sequences can be accessed at NCBI SRA, with accession number SRA092047. The rest of our analysis was initiated from the FASTQ files provided by Illumina's downstream analysis CASAVA software suite.
Ilyas M., Kim J.S., Cooper J., Shin Y.A., Kim H.M., Cho Y.S., Hwang S., Kim H., Moon J., Chung O., Jun J., Rastogi A., Song S., Ko J., Manica A., Rahman Z., Husnain T, & Bhak J. (2015). Whole genome sequencing of an ethnic Pathan (Pakhtun) from the north-west of Pakistan. BMC Genomics, 16(1), 172.
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6

High-coverage Parental Genome Sequencing

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Libraries from high-quality genomic DNA from both parental lines, BC1329 and BC9102, were constructed using the Illumina TruSeq DNA preparation kit, following the manufacturer's instructions (Illumina). Whole-genome resequencing (2 x 150 bp) was performed at the Novogene facility (Novogene Co., Ltd, Hong Kong) using the Illumina HiSeq 2000 sequencing platform. The coverage of the parental lines ranged from 77.6× (BC1329, 102.6 Gb) to 83.8× (BC 9102, 112.4 Gb)
Raman H., Raman R., Pirathiban R., McVittie B., Sharma N., Liu S., Qiu Y., Zhu A., Killian A., Cullis B.R., Farquhar G.D., Williams H.S., White R.G., Tabah D., Easton A.J., & Zhang Y. (2021). Multi-environment QTL analysis delineates a major locus associated with homoeologous exchanges for water-use efficiency and seed yield in allopolyploidBrassica napus.
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