α cask

For application in western blot experiments, dilutions of primary antibodies were prepared in TBS-T with 5% milk powder. The following antibodies were used: α-CASK (rb; 1:1000; Cell Signaling Technologies #9497S); α-HA (ms; 1:1000; Sigma #H9658), α-myc (ms; 1:1000; Sigma #5546); α-Veli 1/2/3 (rb; 1:1000; Synaptic Systems, 184 002); α-SAP97 (ms; 1:500) was provided by Dr. Stefan Kindler (Inst. for Human Genetics, UKE Hamburg). The HRP-coupled secondary antibodies gt-α-rb and gt-α-ms were purchased from ImmunoReagents, Inc. and diluted 1:5000 in TBS-T for use in western blot analysis.

For Western blotting experiments, all primary antibodies were diluted 1:1,000 in TBS-T with 5% milk powder (MP). The following antibodies were used: α-CASK (Rb, #9497S; Cell Signaling Technologies), α-GFP (Ms, #MMS-118P; Covance), α-Veli 1/2/3 (Rb, #184 002; Synaptic Systems), α-HA (Ms, #H9658; Sigma-Aldrich), and α-Myc (Ms, #M5546, Sigma-Aldrich). HRP-coupled secondary antibodies were used in a dilution of 1:2,500 in TBS-T (Gt-α-Ms or Gt-α-Rb, #BOT-20400 or #BOT-20402; ImmunoReagents). For application in immunocytochemistry of hippocampal neurons, two primary antibodies, both prepared in 1:1,000 dilutions in 2% horse serum (HS) in PBS, were used: α-MAP2 (Ck, #ABIN 111 291; Antibodies Online) and α-vGlut1 (Rb, #135 303; Synaptic Systems). As secondary antibodies we used Alexa-405 (Gt-α-Ck, #ab175675; Abcam) and Alexa-633 (Gt-α-Rb, #A-21071; Thermo Fisher Scientific).