The A. baumannii strain MDR-ZJ06 was isolated from the bloodstream of a patient in Hangzhou, China in 2006 and used as the parental isolate [23 (link)]. All isolates mentioned in this study were cultured in Mueller–Hinton (MH) agar plates or broth (Oxoid, Hampshire, UK) and Luria–Bertani (LB) broth (Sangon Biotech, Shanghai, China) at 37°C overnight. The MIC of tigecycline was determined using the broth microdilution method with cation-adjusted MH broth (CAMHB) (Oxoid, Hampshire, UK). Because there is no tigecycline breakpoint for Acinetobacter, the MICs were interpreted following the guidelines of the US Food and Drug Administration for Enterobacterales (with MICs <=2 µg/mL denoting susceptibility and >=8 µg/mL denoting resistance). Other antibiotic MICs were determined using the agar plate dilution method: cefoperazone/sulbactam (2:1), imipenem, meropenem, ceftazidine, cefepime, amikacin, gentamicin and ciprofloxacin. The MIC values were interpreted according to the manufacturer’s instructions. The results were interpreted according to the guidelines recommended by the Clinical and Laboratory Standards Institute (CLSI M100-S29) [24 ].
Mh agar plates or broth
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MH) agar plates or broth are microbiological media used for the cultivation and isolation of a variety of bacterial species. They provide the necessary nutrients and growth conditions for bacterial growth. The core function of these products is to support the growth and identification of bacteria in clinical, environmental, or research settings.
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Lab products found in correlation
2 protocols using mh agar plates or broth
1
Determining Antibiotic Susceptibility of A. baumannii
2
Antibiotic Susceptibility Profiling of P. mirabilis
Check if the same lab product or an alternative is used in the 5 most similar protocols
P. mirabilis strain XH1653 was isolated in October 2015 from a urine sample of a 49-year-old male patient in a hospital in Zhejiang province, China. All isolates used in this study (Table 2 ) were cultured in MH agar plates or broth (Oxoid, Hampshire, United Kingdom) and Luria-Bertani (LB) broth (Sangon Biotech, Shanghai, China) at 37°C. The following 21 compounds were tested using the BD Phoenix 100 automated microbiology system (Becton, Dickinson, MD, USA): imipenem, meropenem, gentamicin, amikacin, cefazolin, ceftazidime, cefotaxime, cefepime, ampicillin, piperacillin, amoxicillin-clavulanate, ampicillin-sulbactam, piperacillin-tazobactam, trimethoprim-sulfamethoxazole, ciprofloxacin, chloramphenicol, levofloxacin, moxifloxacin, aztreonam, tetracycline, and colistin. Susceptibility of XH1653, EC600, XH1814, and XH1815 to antibiotics (imipenem, meropenem, gentamicin, cefepime, ciprofloxacin, tetracycline, and trimethoprim-sulfamethoxazole) was also determined by the broth microdilution method. The results of susceptibility testing were interpreted according to the Clinical and Laboratory Standards Institute (CLSI) guidelines (39 ). E. coli ATCC 25922 served as a control strain.
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