For immunological monitoring, blood samples were taken from the prostate GVAX/ipilimumab-treated patients at follow-up, which was 4 weeks after the last prostate GVAX injection and 8 weeks after the last ipilimumab administration. Blood samples of 14 patients out of the 23 who received docetaxel after GVAX/ipilimumab were available for analysis, whereas blood samples of 11 out of 13 patients who were treated with mitoxantrone could be analyzed. PBMC's were isolated by density centrifugation (Nycomed AS, Oslo, Norway), after which they were either immediately stained and assessed by flow cytometry or cryopreserved for later analysis.
Density centrifugation
Manufactured by Takeda Pharmaceuticals
Sourced in Norway
Density centrifugation is a laboratory technique used to separate and purify particles, cells, or macromolecules based on their buoyant density. It involves the use of a centrifuge to create a density gradient, which allows the sample components to be separated into distinct bands or layers.
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Lab products found in correlation
2 protocols using density centrifugation
1
Immunological Monitoring of Prostate Cancer Patients
2
Cryopreserved PBMC Immune Monitoring
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For immune monitoring, heparinized blood samples were taken from the patients before start of therapy [evaluation 1 (E1)], at the day of the third vaccination [evaluation 2 (E2)] and 5–12 weeks after the third vaccination [evaluation 3 (E3)] (see Fig. 1 for treatment and evaluation scheme). PBMC were isolated by density centrifugation (Nycomed AS, Oslo, Norway) and subsequently cryopreserved for later analysis as previously described [25 (link)].
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