The experiment was conducted under glasshouse conditions. A protocol for chicory grafting was established as depicted in Fig. S3. Seeds were germinated in the dark for 5 d to promote stem growth. The seedlings were subsequently transferred to a 16 h : 8 h, light : dark, photoperiod for 1 month before grafting. Cotyledons and leaves were removed and plantlets used for grafting by the cleft method. The cuttings for the grafting were performed with a razor blade under a binocular stereoscopic microscope. The junctions were covered with a porous tape (Micropore TM ; 3M, Neuss, Germany) and placed high above the substrate in 3-l pots. A humid environment was created by covering the pots with transparent bags that were removed after 14 d. Ammonium nitrate (100 mg) was added to each pot 1 month after grafting. Plants were harvested after 3 months; leaves and storage roots were collected, immediately frozen in liquid N 2 , and then freeze-dried for use in biochemical and molecular analyses. Analyses were performed on three to eight biological replicates for each grafting combination.
Micropore
Manufactured by 3M
Sourced in United States, Sao Tome and Principe
Micropore is a lab equipment product manufactured by 3M. It is a versatile, high-quality membrane filter used for the filtration of aqueous solutions and suspensions. The product serves as a core component in various laboratory applications that require precise filtration and separation of materials.
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Lab products found in correlation
Hp ti sa deep see laser system, by Spectra-Physics (2 mentions)
Celltracker green cmfda, by Thermo Fisher Scientific (2 mentions)
Volocity software, by PerkinElmer (2 mentions)
Fv1000 aom multiphoton system, by Olympus (2 mentions)
Steri strip, by 3M (1 mentions)
Tegaderm, by 3M (1 mentions)
Dexamethasone (dex), by Merck Group (1 mentions)
Ms salt, by Duchefa Biochemie (1 mentions)
Isoflurane, by Henry Schein (1 mentions)
Certified molecular biology agarose, by Bio-Rad (1 mentions)
Balanced salt solution, by Alcon (1 mentions)
17 protocols using micropore
1
Grafting Chicory for Biochemical Analysis
2
Evaluating Endotracheal Tube Taping Methods
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There is no similar study from which to calculate sample size. A convenience sample of 50 volunteers per taping method was planned. The Canadian pediatric anesthesia community was informally polled regarding the most used ETT taping methods via the Canadian Pediatric Anesthesia Society listserv. The most reported taping methods, including various tapes with or without supplementary adhesives, formed the basis for our choice of studied ETT securing methods. The study tapes included were Elastoplast TM , Transpore Clear TM , Transpore Cloth TM , Medipore TM , Micropore TM , and Cloth Adhesive TM (all products of 3M Maplewood, St Paul MN, USA). The supplementary adhesives were none, Cavilon TM (3M Maplewood, MN), USA, tincture of benzoin (Rougier, Ulm, Germany), and MastisolÒ (Ferndale Laboratories, Ferndale, MI, USA). Each tape type was tested with and without each supplementary adhesive for a total 24 tested combinations.
3
Tolerance Studies for Microneedle Arrays
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Tolerance studies using the PMVE/MA MN arrays were carried out prior to the commencement of in vivo experiments, in order to determine whether the formulation or the occlusive dressing to be used in subsequent experiments caused any irritation to the rats. Four MN arrays or pieces of occlusive dressings alone were applied using gentle finger pressure to the shaved backs of each of 2 rats and secured in place for 24 h with medical tape (Micropore®, 3 M, St. Paul, MN, USA). These were then removed following 24 h and the rats were monitored for any signs of irritation on their skin and any other adverse effects for up to 7 days post removal of the MN and/or occlusive dressing.
4
Sticky Tape Test for Forelimb Functional Impairment
5
Post-Operative Lip and Nasal Wound Care
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We usually apply an antibiotic ointment to the suture sites, cover the vermilion and lip with padding gauze and compress the vermilion and lip with paper tape (Micropore; 3M, St. Paul, MN, USA). In wide cleft cases, we apply a Logan’s bow for decreasing the tension on the open dressing lip suture sites, moisturize the dressing frequently with ointment and cover the nasal tip and ala with paper tape. We remove the stitches in 5 to 7 postoperative days. We apply Steri-Strip (3M) during the 3 months after surgery in order to prevent formation of a wide scar, and the patient wears a nostril conformer for 6 months after surgery.
6
Finger Amputation Wound Management
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In the emergency room, the affected finger is put under regional anesthesia with the conventional technique, with 2% simple lidocaine. The wound is irrigated with sterile saline solution and gently debrided with sterile gauze. Amputations with exposure of the distal phalanx were remodeled with gouge and rasp at the level of the edge of the pulp. Ketanserin gel (Sufrexal, Janssen-Cilag, Beerse, Belgium) was applied throughout the extent of the amputation; then, a semipermeable adhesive membrane (Tegaderm, 3M, Saint Paul, MN, United States) was applied to cover the defect. Finally, the entire finger circumference from the distal interphalangeal to the metacarpophalangeal joints was covered with sterile gauze and fixed with surgical tape (Micropore, 3M), and a tubular elastic net was fixed to the base of the affected finger. Every patient received prophylactic oral antibiotic and tetanus toxoid reinforcement vaccination. The patients were evaluated after 3 to 5 days in an outpatient clinic, where they underwent wound cleaning by irrigation with sterile saline solution without debridement; then, ketanserin gel was reaplied, and the wound was covered again with a new semipermeable adhesive membrane, a procedure repeated weekly until epithelialization of the defect was achieved.
7
Microcosm Incubation for Fungal Biomass
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Each plate was sealed with surgical tape (Micropore, 3M, Minnesota, United States), which allowed gas exchange, while limiting water evaporation. The two-compartment microcosms were incubated for 2 weeks in a dark climate chamber at 21°C. After 2 weeks, the sand and soil were sampled separately from all microcosms. For sand, 0.5 g samples were obtained from a 2 mm layer close to the membrane filter. These samples were used for ergosterol-based fungal biomass determination, while the rest of the sand present in the inserts was used to measure the moisture content. Similarly, 1 g samples of soil were taken from the 2 mm layer right below the filter. Half of this sample was used to determine fungal biomass with ergosterol and half was stored at −20°C to be used for DNA extraction. Samples for ergosterol extraction were stored in methanol KOH 4% at −20°C. Soil moisture was determined by using the rest of the soil in the lower compartment. Moisture content of sand and soil were measured as weight loss following oven-drying at 105° C overnight.
8
Intravital Imaging of Neutrophil Migration
9
Petri dish trapping experiment for plant-fungus interactions
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A trapping experiment with round tripartite Petri dishes (Y‐plates, 94 mm x 15 mm; Greiner Bio-One International GmbH, Kremsmünster, Austria; Kai and Piechulla, 2009 (link)) was set up according to the above-described split-plate assays with three biological replicates per treatment. Five Col-0 plants were transferred to a compartment filled with 7 ml ½ MS without sucrose and Serendipita isolate 30 was grown on either 7 ml PDA or 7 ml ½ MS supplemented with 1% (w/v) sucrose. The third compartment was filled with 7 ml 0.1 M Ba(OH)2, which reacts with CO2 to form a BaCO3 precipitate. After 8 days, shoot FW was determined.
Alternatively, split plates with two compartments were sealed with different types of tape: plastic foil blocking all gas exchange, a polyurethane-based adhesive strip allowing exchange of O2 and CO2 (Breathe-Easy film, Diversified Biotech, Inc., Boston, MA, USA), and a completely air permeable surgical tape (Micropore, 3M, St. Paul, MN, USA). Per isolate-sealing tape combination, three replicates were included and after 10 days of VOC exposure the Col-0 shoot FW was measured.
Alternatively, split plates with two compartments were sealed with different types of tape: plastic foil blocking all gas exchange, a polyurethane-based adhesive strip allowing exchange of O2 and CO2 (Breathe-Easy film, Diversified Biotech, Inc., Boston, MA, USA), and a completely air permeable surgical tape (Micropore, 3M, St. Paul, MN, USA). Per isolate-sealing tape combination, three replicates were included and after 10 days of VOC exposure the Col-0 shoot FW was measured.
10
Unilateral Forelimb Constraint in Neonatal Mice
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Neonatal mice underwent unilateral forelimb constraint for a month beginning on P1; the constraint was removed on P30 (Fig 1A ). The right forelimb was bandaged to the chest using surgical tape (3M Micropore). The neonates were returned to their mothers and checked twice daily; reapplication of the bandage was carried out as needed. As the mice grew we reinforced the surgical tape with tissue adhesive (3M™ Vetbond™). The constraint was removed at one month of age and animals recovered for 2 weeks or longer before their performance was tested on a battery of motor tasks. At the age of behavioral testing and euthanasia there were no differences in body weight between constrained wild type and EphA4 mice (see S1 File ).
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