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Goat polyclonal anti actin

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Goat polyclonal anti-actin is a laboratory reagent used to detect and quantify the presence of the actin protein in biological samples. It is produced by immunizing goats with actin and collecting the resulting polyclonal antibodies.

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Lab products found in correlation

Hybond ecl membrane, by Cytiva (1 mentions) Modified bradford assay, by Bio-Rad (1 mentions) Penicillin and streptomycin, by Thermo Fisher Scientific (1 mentions) Trypsin edta, by Thermo Fisher Scientific (1 mentions) Lps escherichia coli 0127 b8, by Merck Group (1 mentions) Rabbit polyclonal anti tgf β1, by Abcam (1 mentions) 5 and 6 chloromethyl 2 7 dichlorodihydrofluorescein diacetate acetyl ester cm h2dcfda, by Thermo Fisher Scientific (1 mentions) Rabbit monoclonal anti erk1 2, by Cell Signaling Technology (1 mentions) Rabbit polyclonal anti akt, by Cell Signaling Technology (1 mentions) Rabbit monoclonal anti phosphorylated akt, by Cell Signaling Technology (1 mentions) Mouse monoclonal anti e cadherin, by BD (1 mentions) Polyclonal rabbit anti trip 1, by Abcam (1 mentions) Rabbit polyclonal anti ubiquitin, by Cell Signaling Technology (1 mentions) Mouse monoclonal anti γ tubulin, by Merck Group (1 mentions) Mouse monoclonal anti lamin a c, by Santa Cruz Biotechnology (1 mentions) Mouse monoclonal anti oct3 4, by Santa Cruz Biotechnology (1 mentions) Rabbit monoclonal anti p perk, by Cell Signaling Technology (1 mentions) Mouse monoclonal anti vinculin, by Merck Group (1 mentions) Anti p62, by Merck Group (1 mentions) Mouse monoclonal anti α tubulin, by Merck Group (1 mentions)

7 protocols using goat polyclonal anti actin

1

Western Blot Analysis of SDHD Protein

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Cells were lysed for 15 min at 4°C using RIPA buffer (1% NP-40 in 150 mM NaCl, 50 mM Tris [pH 7.5], 2 mM EDTA) containing phosphatase and protease inhibitors. Proteins were quantified using a modified Bradford assay (Biorad). Protein samples (50 μg) were separated in 10% SDS/PAGE gels and electroblotted to Hybond ECL membrane (Amersham Biosciences). SDHD (polyclonal, rabbit, 1:200, Santa Cruz Biotechnology) was used. Secondary antibody was conjugated with peroxidase (Santa Cruz Biotechnology) and visualized by the ECL detection solution. Membrane was re-stained with a goat polyclonal anti-actin (Santa Cruz Biotechnology) for loading protein control. XTC1, a hurthle cell thyroid cell line, was used as positive control.
Pópulo H., Batista R., Sampaio C., Pardal J., Lopes J.M, & Soares P. (2017). SDHD promoter mutations are rare events in cutaneous melanomas but SDHD protein expression is downregulated in advanced cutaneous melanoma. PLoS ONE, 12(6), e0180392.
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2

Reagents and Antibodies for Cell Culture

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Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), and 0.25% trypsin-EDTA, penicillin and streptomycin (PenStrep) were purchased from Invitrogen (Carlsbad, CA, USA). 5-(and-6)-chloromethyl-2,7-dichlorodihydrofluoresceindiacetate, acetyl ester (CM-H2DCFDA) was obtained from Molecular Probe (Invitrogen). LPS (Escherichia coli 0127:B8) was purchased from Sigma-Aldrich (St. Louis, MO). The following primary antibodies were used in this study: rabbit monoclonal anti-Nox4,rabbit polyclonal anti-TNFα, rabbit polyclonal anti-TGFβ1 (Abcam, Cambridge, MA, USA); rabbit polyclonal anti-Nox4 (Millipore, Billerica, MA, USA); rabbit polyclonal anti-NFĸB p65, rabbit polyclonal anti-PCNA, goat polyclonal anti-GAPDH, goat polyclonal anti-actin (Santa Cruz Biotechnology, CA, USA); rabbit monoclonal anti-ERK1/2, rabbit monoclonal anti-MyD88 (Cell Signaling Technology, MA, USA); mouse monoclonal Anti-ERK1/2 (pT202/pY204) (BD Biosciences, USA) and rabbit polyclonal anti-NFĸB p65(Ser536) (Bioss Antibodies, USA).
Singh A., Koduru B., Carlisle C., Akhter H., Liu R.M., Schroder K., Brandes R.P, & Ojcius D.M. (2017). NADPH oxidase 4 modulates hepatic responses to lipopolysaccharide mediated by Toll-like receptor-4. Scientific Reports, 7, 14346.
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3

Western Blot Analysis of Lung and Cell Lysates

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Total lung or cell lysates were prepared using previously described methods (Navarro et al. 2009; Perez et al. 2011; Nyp et al. 2014). Western blots were developed using ECL (chemiluminescence) with the following antibodies: polyclonal rabbit anti‐TRIP‐1 (1:1000, Abcam); mouse monoclonal antismooth muscle actin (1:1000) and tubulin (1:10,000) (Sigma‐Aldrich); rabbit monoclonal antiphosphorylated Akt (1:1000) and rabbit polyclonal anti‐Akt (1:1000) (Cell Signaling); mouse monoclonal anti‐E‐cadherin (1:1000, BD Biosciences); and goat polyclonal antiactin (1:2000, Santa Cruz Biotechnologies).
Nyp M.F., Mabry S.M., Navarro A., Menden H., Perez R.E., Sampath V, & Ekekezie I.I. (2018). Lung epithelial‐specific TRIP‐1 overexpression maintains epithelial integrity during hyperoxia exposure. Physiological Reports, 6(5), e13585.
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4

Immunofluorescence and Immunoblotting Antibodies

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Primary antibodies used for immunofluorescence and immunoblotting were rabbit antiserum to LAP2α (245.2) (Vlcek et al. 2002 (link)), mouse monoclonal anti-LAP2α (15/2) (Vlcek et al. 2002 (link)), mouse monoclonal antibody to LAP2α (hybridoma supernatant) (Alexis Biochemicals, 8C10-1H11), goat polyclonal anti-Lamin A/C (Santa Cruz Biotechnology, N-18), mouse monoclonal anti-Lamin A/C (clone 4C11, provided by E. Ogris, Max F. Perutz Laboratories, Vienna, Austria) (Roblek et al. 2010 (link)), rabbit polyclonal anti-lamin A (Dechat et al. 2007 (link)), and rabbit polyclonal anti-lamin C (kindly provided by R. Goldman, Northwestern University, Chicago, IL) (Kochin et al. 2014 (link)), mouse monoclonal anti-progerin (clone 13A4, provided by E. Ogris) (Alexis Biochemicals), monoclonal mouse anti-myc (provided by E. Ogris) (Alexis Biochemicals), rabbit polyclonal anti-ubiquitin (Cell Signaling, #3933), mouse monoclonal anti-Col12A1 (Santa Cruz Biotechnology, A-11), goat polyclonal anti-Col1A1 (Santa Cruz Biotechnology, D-13), rabbit monoclonal anti-TIMP2 (Cell Signaling), goat polyclonal anti-actin (Santa Cruz Biotechnology, I-19), and mouse monoclonal anti-γ-tubulin (Sigma, GTU-88).
Vidak S., Kubben N., Dechat T, & Foisner R. (2015). Proliferation of progeria cells is enhanced by lamina-associated polypeptide 2α (LAP2α) through expression of extracellular matrix proteins. Genes & Development, 29(19), 2022-2036.
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5

Western Blotting Antibody Dilutions

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Antibodies were used at the following dilutions: rabbit polyclonal anti-ATF6, 1:500 (Abcam, catalog number ab37149); mouse monoclonal anti-BIP, 1:1000 (BD, catalog number 610978); rabbit monoclonal anti-p-PERK, 1:1000 (Cell Signaling Technology, catalog number 3179); rabbit polyclonal anti-p-EIF2A, 1:1000 (Cell Signaling Technology, catalog number 9721); rabbit monoclonal anti-EIF2A, 1:1000 (Abcam, catalog number ab169528), rabbit polyclonal anti-PA28α, 1:1000 (ENZO, catalog number BML-PW8185); rabbit polyclonal anti-β5i, 1:1000 (ENZO, catalog number BML-PW8400); goat polyclonal anti-ACTIN, 1:1000 (Santa Cruz Biotechnology, catalog number sc-1616); mouse monoclonal anti-OCT3/4, 1:1000 (Santa Cruz Biotechnology, catalog number sc-5279); rabbit monoclonal anti-GFP, 1:1000 (Cell Signaling Technology, catalog number 2956); Mouse monoclonal Anti-VINCULIN, 1:10,000 (Sigma, catalog number V9131); rabbit polyclonal anti-XBP1, 1:1000 (Abcam, catalog number ab37152).
Xiao R., Xu D., Zhang M., Chen Z., Cheng L., Du S., Lu M., Zhou T., Li R., Bai F, & Huang Y. (2024). Aneuploid embryonic stem cells drive teratoma metastasis. Nature Communications, 15, 1087.
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6

Antibody Detection of TDPs and Related Proteins

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The following primary antibodies were used: HRP-conjugate anti-GFP antibody (Vector Laboratories, Burlingame, CA, USA; MB-0712) for TDPs detection in FRA and WB (1:10,000 for NSC34; 1:2,000 for C2C12); mouse monoclonal anti-FLAG (Sigma-Aldrich; F1804) for TDPs detection in FRA (1:2000) and WB (1:1,000); mouse monoclonal anti-TDP-43 (Proteintech; 12892) (1:1,000); home-made rabbit polyclonal anti-HSPB8 (1:2,000); home-made polyclonal anti-Bag3 (1: 3,000); rabbit polyclonal anti-HA (Santa Cruz Biotechnology; sc-7392) (1:500); goat polyclonal anti-ACTIN (Santa Cruz Biotechnology; sc-1616) (1:1,000); rabbit polyclonal anti-GAPDH (Santa Cruz Biotechnology; sc-25778) (1:1,000); rabbit polyclonal anti-p62 (Sigma-Aldrich; P0067) (1:2,000); rabbit polyclonal anti-LC3 (Sigma-Aldrich; L8918) (1:2,000); mouse monoclonal anti-α-tubulin (Sigma-Aldrich; T6199) (1:2,000).
The following secondary antibodies were used: goat anti-rabbit HRP-conjugate secondary antibody (Santa Cruz Biotechnology; sc-2004); goat anti-mouse HRP-conjugate secondary antibody (Santa Cruz Biotechnology; sc-2005); donkey anti-goat HRP-conjugate secondary antibody (Santa Cruz Biotechnology; sc-2020).
Cicardi M.E., Cristofani R., Rusmini P., Meroni M., Ferrari V., Vezzoli G., Tedesco B., Piccolella M., Messi E., Galbiati M., Boncoraglio A., Carra S., Crippa V, & Poletti A. (2018). Tdp-25 Routing to Autophagy and Proteasome Ameliorates its Aggregation in Amyotrophic Lateral Sclerosis Target Cells. Scientific Reports, 8, 12390.
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7

Immunohistochemical Staining Protocol

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The following primary antibodies were used: goat polyclonal anti-AQP4 (Santa Cruz, CA, USA); goat polyclonal anti-CD31 (Santa Cruz, CA, USA); rabbit polyclonal anti-GFAP (Sigma-Aldrich, Milan, Italy); mouse monoclonal anti-GFAP (clone G-A-5, Millipore, Merck KGaA, Darmstadt, Germany); mouse monoclonal anti-GAPDH (MAB 374, Millipore, Merck KGaA, Darmstadt, Germany); mouse monoclonal anti-Glutamine Synthetase (MAB 302, Millipore, Merck); goat polyclonal anti-actin (Santa Cruz, CA, USA). The following secondary antibodies (all from Invitrogen, Milan, Italy) were used for immunofluorescence: donkey anti-goat Alexa Fluor488; donkey anti-mouse Alexa Fluor488; donkey anti-rabbit Alexa Fluor594; donkey anti-goat Alexa Fluor594; donkey anti-rabbit Alexa Fluor647. The following secondary antibodies (all from Santa Cruz, CA, USA) were used for Western blot:
goat anti-mouse IgG-horseradish peroxidase (HRP); goat anti-rabbit IgG-HRP; donkey anti-goat IgG-HRP.
Nicchia G.P., Pisani F., Simone L., Cibelli A., Mola M.G., Dal Monte M., Frigeri A., Bagnoli P, & Svelto M. (2016). Glio-vascular modifications caused by Aquaporin-4 deletion in the mouse retina. Experimental eye research, 146.
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