Antibodies used in this study were listed in Supplementary Table S1 . ECL-plus kit was from NCM Biotech in China. The Chloroquine (CQ) was purchased from Sigma–Aldrich Chemical Company (St. Louis, MO, USA). And CC90003, DC661, N-acetyl-L-cysteine (NAC), Sulforaphane (SFN) were obtained from Selleck Chemicals (Houston, TX, USA). Lipofectamine 2000 was purchased from Invitrogen (USA).
N acetyl l cysteine
Manufactured by Selleck Chemicals
Sourced in United States
N-acetyl-L-cysteine is a chemical compound that serves as a source of the amino acid cysteine. It is commonly used in various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
2 chloroethanol 2 ce, by Sinopharm (2 mentions)
Qpcr assay kits, by Takara Bio (2 mentions)
Gibh 130 gi, by Merck Group (2 mentions)
Short hairpin rna shrna, by Hanbio Biotechnology (2 mentions)
Fluorocitrate fc, by Merck Group (2 mentions)
Small interfering rnas sirnas, by RiboBio (2 mentions)
Sb202190, by Selleck Chemicals (2 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Chloroquine, by Merck Group (1 mentions)
Dc661, by Selleck Chemicals (1 mentions)
Sulforaphane, by Selleck Chemicals (1 mentions)
Nec 1, by Selleck Chemicals (1 mentions)
Skov3, by American Type Culture Collection (1 mentions)
Ovcar3, by American Type Culture Collection (1 mentions)
Thp 1, by American Type Culture Collection (1 mentions)
Phorbol 12 myristate 13 acetate, by Merck Group (1 mentions)
A2780, by American Type Culture Collection (1 mentions)
Rotenone, by Merck Group (1 mentions)
Dulbecco s modified eagle s medium f12 dmem f12, by Thermo Fisher Scientific (1 mentions)
Recombinant mouse il 1β, by R&D Systems (1 mentions)
7 protocols using n acetyl l cysteine
1
Antibody-Based Cellular Assay Reagents
2
Radiation-Induced Cell Damage Mitigation
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Cells were irradiated with a single dose γ-ray of 10 and 20 Gy (68.31 cGy/min), and then the medium was replaced or added with Nec-1 (20 μM) (S8037, Selleck, Houston, TX), N-acetyl-l -cysteine (NAC) (20 μM) (S1623, Selleck), or Alexidine (AD, 50 μM) (1715-30-6, Yuanye Biotechnology, Shanghai, China) after irradiation. All these reagents were first dissolved in DMSO to 10 mM and then diluted in DMEM. Table 1 contains detailed information including validation references of these reagents.
3
Comparative Analysis of Ovarian Cancer Cell Lines
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Human ovarian cancer cell line A2780, Ovcar-3, and SKOV-3, murine ovarian cancer cell line ID8, and human peripheral blood monocytes THP-1 were purchased from the American Type Culture Collection (ATCC). Peripheral blood mononuclear cells (PBMC) were purchased from Stemcell Technologies. All cell lines were cultured in RPMI 1640 or in Dulbecco’s modified Eagle’s (DMEM) media supplemented with 10% FBS. All cell lines did not have mycoplasma contamination determined by RT-PCR. N-acetyl-L-cysteine (NAC) was purchased from Selleckchem, and phorbol 12-myristate 13-acetate (PMA), catalase-PEG, and rotenone were from Sigma Aldrich. The sources of antibodies used in immunoblotting, flow cytometry, and IHC were shown in Suppl. Table 2 .
4
Antioxidant and Anti-inflammatory Protocol
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Astaxanthin (purity 99.54%), ERK1/2 inhibitor PD0325901, and ROS inhibitor N-acetyl-L-cysteine (NAC) were purchased from Selleck Chemicals, and were dissolved in 0.1% dimethylsulfoxide (DMSO). NF-kB inhibitor QNZ was supplied by MCE. Recombinant mouse IL-1β, and recombinant mouse TNF-α were purchased from R&D systems (Minneapolis, MN, USA). TBHP was purchased from Sigma-Aldrich (St. Louis, MO, USA). Fetal bovine serum (FBS) and Dulbecco’s modified Eagle’s medium F12 (DMEM /F12) were supplied by Gibco (NY, USA).
5
Antibody-based Detection of Apoptosis
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Antibody against cleaved caspase 3 was obtained from Cell Signaling Technology (Danvers, MA, USA). Antibody against xCT was purchased from Sangon Biotech (Shanghai, USA). Antibody against β-actin and tubulin were purchased from Bioworld Technology (Philadelphia, PA, USA). Anti-rabbit IgG (H+L) affinity purified secondary antibody was obtained from Vector Laboratories (Burlingame, CA, USA). Artesunate, dihydroartemisinin, N-Acetyl-L-cysteine and ferrostatin-1were acquired from Selleck (Houston, TX, USA). RPMI-1640 medium was purchased from HyClone (Waltham, MA, USA). Fetal bovine serum (FBS) was purchased from Biological Industries (Cromwell, CT, USA). 2′,7′-Dichlorofluorescin diacetate was purchased from Sigma-Aldrich (St. Louis, MO, USA).
6
Signaling Pathways Modulating Inflammatory Response
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2-Chloroethanol (2-CE) was obtained from Sinopharm Chemical Reagent Co., Ltd. (Ningbo, China), while reagents for the cell culture were purchased from Biological Industries (Beit-Haemek, Israel). The quantitative polymerase chain reaction (qPCR) assay kits were acquired from Takara (Japan). SB202190, pyrrolidine dithiocarbamate (PDTC), SR11302, and N-acetyl-l-cysteine (NAC) were purchased from Selleck (Houston, USA), APExBIO (Houston, USA), or Beyotime Biotechnology (Shanghai, China). Fluorocitrate (FC) and GIBH-130 (GI) were bought from Sigma (New Jersey, USA) and MedChemExpress (New Jersey, USA), respectively. Short hairpin RNA (shRNA) targeting the interleukin-1β (IL-1β) gene was designed and synthesized by Hanbio (Shanghai, China), while small interfering RNAs (siRNAs) targeting the tumor necrosis factor-α (TNF-α), inducible nitric oxide synthase (iNOS), or CYP2E1 genes were designed and synthesized by RiboBio (Guangzhou, China).
7
Reagents and Chemicals for Cell Culture
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2.1. Reagents 2-Chloroethanol (2-CE) was obtained from Sinopharm Chemical Reagent Co., Ltd. (Ningbo, China), while reagents for the cell culture were purchased from Biological Industries (Beit-Haemek, Israel). The quantitative polymerase chain reaction (qPCR) assay kits were acquired from Takara (Japan). SB202190, pyrrolidine dithiocarbamate (PDTC), SR11302, and N-acetyl-l-cysteine (NAC) were purchased from Selleck (Houston, USA), APExBIO (Houston, USA), or Beyotime Biotechnology (Shanghai, China). Fluorocitrate (FC) and GIBH-130 (GI) were bought from Sigma (New Jersey, USA) and MedChemExpress (New Jersey, USA), respectively. Short hairpin RNA (shRNA) targeting the interleukin-1β (IL-1β) gene was designed and synthesized by Hanbio (Shanghai, China), while small interfering RNAs (siRNAs) targeting the tumor necrosis factor-α (TNF-α), inducible nitric oxide synthase (iNOS), or CYP2E1 genes were designed and synthesized by RiboBio (Guangzhou, China).
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