Rabbit anti krt5

Manufactured by Abcam

Rabbit anti KRT5 is a primary antibody that recognizes the keratin 5 protein, which is a structural protein found in certain types of epithelial cells. This antibody can be used for the detection and localization of KRT5 in various biological samples.

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Rabbit anti- (5 citations) Anti-KRT5 (4 citations)

2 protocols using rabbit anti krt5

Immunofluorescence Staining of Lung Cells

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The following antibodies were used: Mouse anti-αSMA-Cy3 (1:1000, Sigma-Aldrich, C6198); Rat anti-CDH1 (1:500, Santa Cruz, sc-59778); Goat anti-KCNJ13 (1:50, Santa Cruz, C19); Rabbit anti-SOX9 (1:400, Millipore, AB5535); Sheep anti-GM130 (1:50, R&D systems, AF8199); Rabbit anti-Ki67 (1:400, Cell Signaling Technologies, #9027); Rabbit anti Cleaved Caspase-3 (1:600, Cell Signaling Technologies, #9661); Rabbit anti KRT5 (1:1000, Abcam, ab53121); Goat anti-CC10 (1:200, Santa Cruz, T-18); Rabbit anti-NKX2.1 (1:400, Santa Cruz, H-190); Rabbit anti-SFTPC (1:400, Millipore, AB3786); Mouse anti-PCNA (1:400, Santa Cruz, sc-56); Rabbit anti-PH3 (1:400, Millipore, 06-570); Rabbit anti-p-AKT^ser473 (1:200, Cell Signaling Technologies, #4060); Rabbit anti-AKT (1:2000 Cell Signaling Technologies, #9272), and Rabbit anti-GAPDH (1:3000, Cell Signaling Technologies, #2118).

Yin W., Kim H.T., Wang S., Gunawan F., Wang L., Kishimoto K., Zhong H., Roman D., Preussner J., Guenther S., Graef V., Buettner C., Grohmann B., Looso M., Morimoto M., Mardon G., Offermanns S, & Stainier D.Y. (2018). The potassium channel KCNJ13 is essential for smooth muscle cytoskeletal organization during mouse tracheal tubulogenesis. Nature Communications, 9, 2815.

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Immunostaining of FFPE Tissue Sections

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FFPE tissues were sectioned at a 5-micron thickness and stained as previously described.(11 (link)) Antibodies used are: rabbit anti-KRT5, mouse anti-KRT5, mouse anti-PTBP3, rabbit anti-c-myc (Abcam); mouse anti-CEACAM5 (ProMab Biotechnologies Inc); rabbit anti-SLC2A1 (Alpha Diagnostic International); anti-rabbit Cy3, anti-mouse Alexafluor 647, anti-mouse Cy3, anti-rabbit Alexafluor 647 (Jackson ImmunoResearch). Immunostained tissues were visualized on an Axiocam system (Zeiss), and images were taken using the Axiovision software.

Ooi A.T., Gower A.C., Zhang K.X., Vick J.L., Hong L., Nagao B., Wallace W.D., Elashoff D.A., Walser T.C., Dubinett S.M., Pellegrini M., Lenburg M.E., Spira A, & Gomperts B.N. (2014). Molecular profiling of premalignant lesions in lung squamous cell carcinomas identifies mechanisms involved in stepwise carcinogenesis. Cancer prevention research (Philadelphia, Pa.), 7(5), 487-495.

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