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Aryacell sorter

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The BD Aryacell sorter is a flow cytometry instrument designed for cell sorting applications. It utilizes fluidics, optics, and electronics to rapidly analyze and sort individual cells within a sample.

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Lab products found in correlation

Bv421 tcrβ clone h57 597, by BioLegend (2 mentions) Percp cy5.5 cd4 clonerm4 5, by BioLegend (2 mentions) Pe cd25 clone pc61, by BioLegend (2 mentions) Bv 711 cd8 clone 53 6.7, by BioLegend (2 mentions)

2 protocols using aryacell sorter

1

Isolation and RNA Extraction of Foxp3+ T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
DEREG (eGFP.Foxp3) reporter mice were used for cell sorting. Single
cell suspensions were obtained from BM and stained with CD16/32,BV
421-TCRβ (clone H57–597), PerCP/Cy5.5-CD4 (clone
RM4–5), PE-CD25 (clone PC61) and BV 711-CD8-(clone 53–6.7)
(Biolegend, San Diego, CA). 1 X106 cells were sorted on a BD Arya
cell sorter. The sorted cells were collected in complete RPMI-1640 media.
Purified cells were then collected by centrifugation and dissolved in Trizol
reagent for RNA isolation.
Tyagi A.M., Yu M., Darby T.M., Vaccaro C., Li J.Y., Owens J.A., Hsu E., Adams J., Weitzmann M.N., Jones R.M, & Pacifici R. (2018). The Microbial Metabolite Butyrate Stimulates Bone Formation via T Regulatory Cell-Mediated Regulation of WNT10B Expression. Immunity, 49(6), 1116-1131.e7.
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2

Isolation and RNA Extraction of Foxp3+ T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
DEREG (eGFP.Foxp3) reporter mice were used for cell sorting. Single
cell suspensions were obtained from BM and stained with CD16/32,BV
421-TCRβ (clone H57–597), PerCP/Cy5.5-CD4 (clone
RM4–5), PE-CD25 (clone PC61) and BV 711-CD8-(clone 53–6.7)
(Biolegend, San Diego, CA). 1 X106 cells were sorted on a BD Arya
cell sorter. The sorted cells were collected in complete RPMI-1640 media.
Purified cells were then collected by centrifugation and dissolved in Trizol
reagent for RNA isolation.
Tyagi A.M., Yu M., Darby T.M., Vaccaro C., Li J.Y., Owens J.A., Hsu E., Adams J., Weitzmann M.N., Jones R.M, & Pacifici R. (2018). The Microbial Metabolite Butyrate Stimulates Bone Formation via T Regulatory Cell-Mediated Regulation of WNT10B Expression. Immunity, 49(6), 1116-1131.e7.
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