6300 ftir spectrometer

All chemical spectral analyses were conducted at Kuwait University Research Center. Thin layer chromatography (TLC) was performed using Polygram SIL G/UV 254 TLC plates, and the results were visualized under ultraviolet light at 254 and 350 nm, with hexane/ethyl acetate as a solvent. Column chromatography was performed using silica gel 60A with a mesh size of 40–60 µm. ¹H and ¹³C nuclear magnetic resonance (NMR) spectra were obtained using a Bruker DPX 600 NMR spectrophotometer at 600 MHz and 150 MHz in DMSO and CDCL3, respectively. Mass spectra were detected on a GC-MS DFS–Thermo spectrometer. An IR spectrum was obtained with a Jasco 6300 FTIR spectrometer. Melting point was determined using a Netzsch DSC 204 F1 Phoenix differential scanning calorimeter.
SKOV3 human ovarian cancer cells were obtained from American Type Culture Collection. A cholesterol-conjugated HSP27 inhibitor was synthesized in our laboratory. Cell culture media and other supplements, such as 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), were obtained from Sigma-Aldrich (Milwaukee, WI). Chemicals and reagents are commercially available and ready for direct use, requiring no preparation. Biological parameters were examined at the Biology Department, Kuwait University.