Cells were seeded into 96-well plates and 100 μL of EdU reagent was added and cultured for 2 h. After washing with PBS (Solarbio), the cells were dyed with Apollo and then washed with methanol (Solarbio). Later, the cells were fixed, discolored and permeated. Subsequently, the cells were cultivated with 100 μL of DAPI reaction solution (Sigma-Aldrich Chemical Company, St Louis, MO, USA) followed by viability determination via a fluorescent microscope (Thermo Fisher Scientific).
Dapi reaction solution
Manufactured by Merck Group
Sourced in United States
DAPI (4',6-diamidino-2-phenylindole) reaction solution is a fluorescent dye used in molecular biology and microscopy applications. It binds strongly to the DNA present in cells, allowing for the visualization and analysis of cellular structures.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using dapi reaction solution
1
Cell Proliferation Assay with EdU
2
Cell Proliferation and DNA Synthesis Assay
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Cell proliferation ability and DNA synthesis were determined using the EdU staining assay. Brie y, the cells were treated with different concentrations of HHT for 48 h. Then the cells in the logarithmic growth phase were seeded on the coverslips (NEST, USA), and added with 10 uM EdU solution (Abcam, USA) to each well for incubation 2 h at 37°C. Subsequently, the cells were xed with 4% paraformaldehyde for 20 min and treated with 2M HCl for 30 min at room temperature. After washing the cells with cold PBS three times, each well was supplemented with 0.5% Triton X-100 (Solarbio, China) and blocked with 10% goat serum (Solarbio, China) for 1 h. For the visualization of nuclei, the cells were stained with DAPI reaction solution (Sigma, USA) for 20 min in dark and then xed with a uorescence quenching agent (Solarbio, China). The EdU-positive cells were nally photographed and counted using a uorescence microscope (Olympus, Japan) under different elds.
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