All materials were used as received. Specifically, 1-methyl-3-pyrrolidinone (97% purity) was purchased from Sigma-Aldrich, FMOC-L-Pro(4-keto)-OH was purchased from Chem-Impex International Inc., and 4-oxo-L-proline hydrobromide (90% purity) was purchased from Alfa Aesar. In addition, methanol (MeOH), acetonitrile (ACN), dimethyl formamide (DMF) and dimethyl sulfoxide (DMSO) were purchased from Acros Organics, hexanes and diethyl ether (Ether) were purchased from Fisher Scientific, and deuterium oxide (D2O; 99.96% purity) was purchased from Cambridge Isotope Laboratories.
4 oxo l proline hydrobromide
Manufactured by Thermo Fisher Scientific
Sourced in United States
4-oxo-L-proline hydrobromide is a chemical compound that serves as a building block for various organic synthesis reactions. It is a white crystalline powder that is soluble in water and organic solvents. This product is commonly used in research and development applications, but a detailed description of its intended use is not available.
Automatically generated - may contain errors
Lab products found in correlation
Histrap ff crude column, by GE Healthcare (2 mentions)
Vivaspin 20 centrifugal concentrator, by Sartorius (2 mentions)
Hiload 16 60 superdex 200, by GE Healthcare (2 mentions)
Hiscreen blue ff, by GE Healthcare (2 mentions)
Q sepharose ff resin, by GE Healthcare (2 mentions)
Hiscreen, by Cytiva (2 mentions)
Hexane, by Thermo Fisher Scientific (1 mentions)
Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (1 mentions)
Deuterium oxide d2o, by Cambridge Isotopes (1 mentions)
Diethyl ether ether, by Thermo Fisher Scientific (1 mentions)
3 protocols using 4 oxo l proline hydrobromide
1
Synthesis of Proline Derivatives
2
Purification of 4-Oxoproline Enzyme
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Reagents of analytical grade, whenever possible, were from Sigma or Merck. 4-oxo-l -proline hydrobromide was purchased from Alfa Aesar (90+% purity) or Fluorochem (95% purity). Q-Sepharose FF resin, HiScreen Blue FF, Superdex 200 16/60 HiLoad, and HisTrap FF crude columns were obtained from GE Healthcare Bio-Sciences. Vivaspin 20 centrifugal concentrators were from Sartorius. Enzymes and DNA-modifying enzymes were obtained from Thermo-Fermentas, A&A Biotechnology, or Bio-Shop.
3
Purification of Recombinant Enzymes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Reagents, of analytical grade whenever possible, were from Sigma or Merck (Darmstadt, Germany). 4-oxo-L-proline hydrobromide was purchased from Alfa Aesar (90+% purity, Ward Hill, USA) or Fluorochem (95% purity, Hadfield, UK). Q-Sepharose FF resin, HiScreen Blue FF, Superdex 200 16/60 HiLoad, and HisTrap FF crude columns were obtained from GE Healthcare Bio-Sciences (Uppsala, Sweden). Vivaspin 20 centrifugal concentrators were from Sartorius (Stockport, UK). Enzymes and DNA modifying enzymes were obtained from Thermo-Fermentas (Waltham, USA), A&A Biotechnology (Gdynia, Poland), or Bio-Shop (Burlington, Canada).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!