RNA was isolated from cells using the RNeasy Mini Kit (Qiagen), or, for RIP experiments, Trizol (Invitrogen), and treated with RNase-free DNase (Qiagen). RNA was reverse transcribed with PrimeScript RT-PCR mix (Clonetech), and Power SYBR Green PCR Master Mix (Applied Biosystems) was used for qPCR. Biological replicates represent independently grown and processed cells. Technical replicates represent multiple measurements of the same biological sample. Primer sequences are provided in Supplementary file 1 .
Primescript rt pcr mix
Manufactured by Takara Bio
PrimeScript RT-PCR mix is a reagent kit designed for reverse transcription and real-time PCR amplification. It contains the necessary components for both steps in a single reaction mixture.
Automatically generated - may contain errors
2 protocols using primescript rt pcr mix
1
RNA Isolation and Quantification Protocol
2
Quantitative RT-PCR Absolute Transcript Quantification
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