Freshly isolated human neutrophils were seeded onto 96-wells plates and pretreated with ADO receptor-specific antagonists including 1 μM ZM241385, 100 nM MRS1754, and 100 nM MRS3777 (#1036, #2752, and #2403; R&D Systems) for 15 min followed by 30 min of ADO or NECA treatment. The cells were then stimulated with PMA, and NET formation was quantified as previously described. Optimal antagonist dose was determined by dose–response curves ranging from 1 nM to 10 μM.
Zm241385
Manufactured by R&D Systems
ZM241385 is a small molecule inhibitor that blocks the activity of the protein kinase CDK7. CDK7 is a component of the transcription factor TFIIH and plays a role in the regulation of RNA polymerase II-mediated transcription.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using zm241385
1
Neutrophil NET Formation Regulation
2
Mitochondrial Function Assay in RAW 264.7 Cells
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Oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) were measured using an XF96 oximeter (Seahorse Biosciences, North Billerica, MA). RAW 264.7 cells were seeded in 96-well assay plates, and cells were pretreated with 8-[4-[4-(4-chlorobenzyl)piperazide-1-sulfonyl)phenyl]]-1-propylxanthine (PSB0788) and 4-(2-[7-amino-2-(2-furyl)[1,2,4]triazolo[2,3-a][1,3,5]triazin-5-ylamino]ethyl)phenol (ZM241385) (R&D Systems) or vehicle at the indicated concentrations. After a 30-min incubation period, 1 μmol/L NECA or vehicle was added. After an additional 30-min incubation period, the cells were stimulated with 250 μmol/L palmitate or vehicle and incubated for 12 h at 37°C in 5% CO2 atmosphere. After 12 h, media were removed, and the cells were washed once with XF assay media and then incubated in a CO2-free atmosphere for 30 min at 37°C in XF assay media.
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