Proteins in equal volumes of affinity chromatography effluents or proteins associated with 700 nM 40S ribosome were resolved by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), transferred to nitrocellulose membranes and probed with antibodies against eIF3d (Abcam, Ab155419), eIF4E (Cell Signaling, #9742), eIF4A (Cell Signaling, #2013), eIF2α (Cell Signaling, #9722), eIF4GI (Cell Signaling, #2858), PABP (Abcam, Ab21060), eIF1A (Abcam, Ab177939), PAIP-1 (Abcam, Ab175211) or ribosomal protein S6 (Cell Signaling, #2317). Membranes were then incubated with species-specific horseradish peroxidase-conjugated secondary antibody (Cell Signaling) followed by detection with ECL substrate (Pierce).
Ab177939
Manufactured by Abcam
Ab177939 is an antibody product offered by Abcam. This product is a recombinant monoclonal antibody.
Automatically generated - may contain errors
Lab products found in correlation
Eif4e, by Cell Signaling Technology (1 mentions)
Eif2α, by Cell Signaling Technology (1 mentions)
Ab21060, by Abcam (1 mentions)
Eif4gi, by Cell Signaling Technology (1 mentions)
Eif4a, by Cell Signaling Technology (1 mentions)
Species specific horseradish peroxidase conjugated secondary antibody, by Cell Signaling Technology (1 mentions)
Anti α tubulin t6199, by Merck Group (1 mentions)
2 protocols using ab177939
1
Affinity Chromatography Protein Profiling
2
Western Blot Protein Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Western blotting was performed as described previously using the following antibodies: anti‐α‐tubulin (T6199; Sigma), anti‐EIF1AX (ab177939; Abcam), anti‐p21 (K0081‐3; MBL), anti‐p53 (sc‐47698; Santa Cruz Biotechnology), anti‐cyclin D1 (2978S; Cell Signaling Technology) and anti‐P‐Rb (8180S; Cell Signaling Technology).
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