Anti his 66005 1 ig

Manufactured by Proteintech

Sourced in China

Anti-His (66005-1-Ig) is a primary antibody that specifically recognizes the histidine (His) tag. It is commonly used in various applications, such as Western blotting, immunoprecipitation, and immunofluorescence, to detect and purify His-tagged recombinant proteins.

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Lab products found in correlation

Protease inhibitor, by Selleck Chemicals (1 mentions) Anti flag 66008 4 ig, by Proteintech (1 mentions) Anti rb ab181616, by Abcam (1 mentions) Anti ha 51064 2 ap, by Proteintech (1 mentions) Protease inhibitor cocktail, by Selleck Chemicals (1 mentions) N ethylmaleimide nem e3876, by Merck Group (1 mentions) Anti senp1 ab108981, by Abcam (1 mentions) Anti ha h6908, by Merck Group (1 mentions) Anti flag agarose a2220, by Merck Group (1 mentions) Anti gapdh, by Cell Signaling Technology (1 mentions) Anti flot2, by Cell Signaling Technology (1 mentions) Protein a g beads, by Selleck Chemicals (1 mentions) Anti stat3 10253 2 ap, by Proteintech (1 mentions)

3 protocols using anti his 66005 1 ig

Comprehensive Antibody and Drug Protocol

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Antibodies and drugs used in this study were anti-YOD1 (PA5-35042), anti- CDK1 (MA5-11472), anti-beta Tubulin (MA5-11732), anti-P70S6K (PA5-28597), and anti-phospho-P70S6K (701,083) (Thermo Fisher Scientific, USA); anti-Ub (ab140601), anti-RB (ab181616), and anti- phospho-Rb (ab173289) (Abcam, USA); anti- Flag(66008-4-Ig), anti- HA (51064-2-AP) and anti-His (66005-1-Ig) (Proteintech, China); Cisplatin (HY-17,394), paclitaxel (HY-B0015), Ro-3306(HY-12,529), CDK1-IN-4(HY-151,408) and Ubiquitin Isopeptidase Inhibitor I, G5(HY-100,738) (MedChemExpress, USA); phosphatase inhibitors and protease inhibitor were obtained from Selleck (USA).

Han Z., Jia Q., Zhang J., Chen M., Wang L., Tong K., He W., Zhang Y., Zhu W., Qin J., Wang T., Liu T., Ma Y., Chen Y., Zha S, & Zhang C. (2023). Deubiquitylase YOD1 regulates CDK1 stability and drives triple-negative breast cancer tumorigenesis. Journal of Experimental & Clinical Cancer Research : CR, 42, 228.

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SUMOylation Protein Interaction Assay

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Anti-HA (H6908, 1:3000), anti-Flag agarose (A2220) and N-ethylmaleimide (NEM; E3876, 20 mM) were purchased from Sigma-Aldrich. Anti-SUMO1 (4940, 1:1000), anti-SAE1 (13585, 1:1000), anti-SAE2 (8688,1:1000) and anti-GAPDH (2118, 1:5000) were purchased from Cell Signaling Technology. Mouse anti-CFL1 (66057-1-Ig, 1:1000), rabbit anti-CFL1 (10960-1-AP, 1:1000), anti-GST (66001-2-Ig, 1:3000) and anti-His (66005-1-Ig, 1:1000) were purchased from Proteintech. Anti-Ubc9 (ab33044, 1:1000) and anti-SENP1 (ab108981, 1:1000) were purchased from Abcam. Anti-HA agarose (26181) and protein A/G agarose (20422) were purchased from Pierce. Protease inhibitor cocktail (B14001) was purchased from Bimake.

Weng W., Gu X., Yang Y., Zhang Q., Deng Q., Zhou J., Cheng J., Zhu M.X., Feng J., Huang O, & Li Y. (2023). N-terminal α-amino SUMOylation of cofilin-1 is critical for its regulation of actin depolymerization. Nature Communications, 14, 5688.

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Protein-Protein Interaction Analysis

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The total protein of cells was harvested with NP-40 solution. The concentration of proteins was measured with the BCA method. Then, relative antibodies were added to the solution, shake the mixture overnight at 4°C. The next day, 20-30 μl protein A/G beads (B23201, Bimake) were added to the solution, shake the mixture for 2 h at 4°C. Magnetic beads were collected, suspended with NP40 lysis and loading buffer, and denatured at 95°C for 5 min. Then the sample solution was collected by the Magnetic Separation Rack. Protein-protein interaction was detected with the Western blot method as mentioned above. The following antibodies were used for studies: anti-FLOT2 (#3426, Cell Signaling Technology); anti-STAT3 (#10253-2-AP, Proteintech); anti-His (#66005-1-Ig, Proteintech).

Xu H., Yin Y., Li Y., Shi N., Xie W., Luo W., Wang L., Zhu B., Liu W., Jiang X, & Ren C. (2023). FLOT2 promotes nasopharyngeal carcinoma progression through suppression of TGF-β pathway via facilitating CD109 expression. iScience, 27(1), 108580.

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