Sprague‐Dawley (CD) rats were purchased from Charles River Laboratories, France (L'Arbresle, France) and C57Bl/6 mice from Janvier Labs (Le Genest‐Saint‐Isle, France). The 8–12‐week‐old NOD/SCID/Il2rg−/− (NSG) mice were bred in our own animal facilities in SPF conditions. Il34LacZ/LacZ mice were kindly provided by Marco Colonna (Washington University, St. Louis, MO).12 Il2rg−/− rats were kindly provided by TRIP Platform (Nantes, France) and have been previously described.37 The animals were housed in a controlled environment (temperature 21 ± 1°C, 12‐h light/dark cycle).
Cd sprague dawley rats
Manufactured by Charles River Laboratories
Sourced in France, United States
The CD Sprague Dawley rats are a widely used animal model in research and preclinical studies. They are a common outbred rat strain known for their robust health and adaptability to various experimental conditions.
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16 protocols using cd sprague dawley rats
1
Animal Sourcing and Housing Protocol
2
Timed Pregnant Sprague-Dawley Rat Model
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Timed pregnant Sprague-Dawley (CD) rats were obtained from Charles Rivers Laboratories and arrived at the Lab Animal Facilities at the University of Mississippi Medical Center on gestational day 11. All animal procedures were approved by the Institutional Animal Care and Use Committee at the University of Mississippi Medical Center. The rats were maintained on a 12 h light/12 h dark cycle and fed standard rodent chow and water ad libitum.
3
Timed Pregnant Sprague-Dawley Rat Model
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Timed pregnant Sprague-Dawley (CD) rats were obtained from Charles Rivers Laboratories and arrived at the Lab Animal Facilities at the University of Mississippi Medical Center on gestational day 11. All animal procedures were approved by the Institutional Animal Care and Use Committee at the University of Mississippi Medical Center. The rats were maintained on a 12 h light/ 12 h dark cycle and fed standard rodent chow and water ad libitum.
4
Timed-Pregnant Rat Gestation Study
5
Rat Superior Cervical Ganglia Dissection
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All animal procedures were approved by the Institutional Animal Care and Use Committee (IACUC) at the University of Pittsburgh. Male and female Sprague Dawley (CD) rats and male SHR rats, aged 7–23 weeks, were obtained from Charles River (Wilmington, MA, United States). After killing rats by CO2 inhalation, superior cervical ganglia were rapidly dissected along with the adjacent carotid artery and transferred to a 60 mm Petri dish coated with Sylgard 184 (Dow Corning, Midland, MI, United States) and filled with Ringer solution for further preparation.
6
Timed Pregnant Sprague-Dawley Rat Study
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Timed pregnant Sprague‐Dawley (CD) rats were obtained from Charles Rivers Laboratories and arrived at the Lab Animal Facilities at the University of Mississippi Medical Center on gestational day 11. All animal procedures were approved by the Institutional Animal Care and Use Committee at the University of Mississippi Medical Center. The rats were maintained on a 12‐hour light/12‐hour dark cycle and fed standard rodent chow and water ad libitum.
7
Sprague-Dawley Rat Housing and Handling
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Thirty-two Sprague-Dawley CD rats (16 males and 16 females, Charles River Laboratories, France) were individually housed under an inverted 12-h light/dark cycle at 21±2°C with food and water available ad libitum. Animals were handled daily for two weeks.
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Sprague Dawley Rat Husbandry Protocol
9
Ovariectomy Rat Model for Osteoporosis
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A schematic detailing the timing of surgical procedures and treatments is shown in figure 1 . All surgical procedures were performed at the same session under isoflurane inhalation anesthesia. 40, 8‐month‐old, skeletally mature, virgin, female CD Sprague‐Dawley rats (Charles River Laboratories, Wilmington, MA) underwent ovariectomy (OVX; n = 20) or sham OVX (SHOVX; n = 20) surgery. The OVX and SHOVX surgeries were performed by Charles River Laboratories. Development of the osteoporotic phenotype occurred over the next 5 weeks.
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Sprague Dawley Rat Husbandry and Handling
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