Plc3 universal leaf cuvette

A CIRAS-3 portable photosynthesis system (PP Systems^®, Hitchin, UK), equipped with a PLC3 universal leaf cuvette, was used to determine photosynthetic capacity (A_max), transpiration (E), and water use efficiency (WUE) in response to increasing levels of photosynthetically active radiation (PAR), expressed as photosynthetic photon flux density (PPFD, 0–1600 µmol m⁻² s⁻¹). Measurements were conducted at 66 DODS and on three leaves per cultivar exposed to WW and DS treatments.

A portable photosynthesis system (CIRAS-3, PP Systems, Amesbury, MA,103 USA) equipped with the leaf chamber fluorometer (PLC3 Universal Leaf Cuvette, 18 × 25 mm window, CFM-3) was used to measure leaf gas exchange parameters, including the net photosynthetic rate (P_n), stomatal conductance (G_s), intercellular CO₂ concentration (C_i), and transpiration rate (T_r). The level of irradiance for measurements was set at 1000 µmol photons m⁻²s⁻¹. The air temperature, CO₂ concentration and relative humidity used were those of the greenhouse conditions. The above and middle fully developed leaves of different treatments were acclimated to the irradiance level approximately 2 min before recording. The measurements were conducted at approximately 10–12 am 8 days before treatment and 4 days, 16 days, 54 days, and 84 days after treatment.