Lactulose (Laevolac) was obtained from Fresenius Kabi Austria, D-Mannitol (Fluka), sucrose and thimerosal were obtained from Sigma Aldrich Austria. Sodium phosphate, dibasic (Na2HPO4), sodium hydroxide, hydrochloric acid (32% m/v), and sodium azide (NaN3) were obtained from VWR International, 3-(trimethylsilyl) propionic acid-2,2,3,3-d4 sodium salt (TSP) from Alfa Aesar (Karlsruhe, Germany), deuterium oxide (2H2O) from Cambridge Isotope laboratories, Inc. (Tewksbury, MA).
Sodium phosphate
Manufactured by Avantor
Sourced in Germany, Poland
Sodium phosphate is a common inorganic compound used in various laboratory applications. It functions as a buffer solution, maintaining a specific pH level in chemical reactions and assays.
Automatically generated - may contain errors
Lab products found in correlation
Sodium azide nan3, by Avantor (5 mentions)
Hydrochloric acid (hcl), by Avantor (5 mentions)
3 trimethylsilyl propionic acid 2 2 3 3 d4 sodium salt tsp, by Thermo Fisher Scientific (5 mentions)
Sodium chloride (nacl), by Avantor (4 mentions)
Deuterium oxide d2o, by Cambridge Isotopes (3 mentions)
Milli q advantage water purification system, by Merck Group (3 mentions)
Deuterium oxide 2h2o, by Cambridge Isotopes (2 mentions)
Methanol, by Avantor (2 mentions)
Thimerosal, by Merck Group (1 mentions)
Sucrose, by Merck Group (1 mentions)
Trehalose, by Merck Group (1 mentions)
Propidium iodide, by Merck Group (1 mentions)
Glycerol, by Merck Group (1 mentions)
Ocrelizumab, by Roche (1 mentions)
Acetonitrile, by Avantor (1 mentions)
Milli q advantage a10, by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Merck Group (1 mentions)
Acetic acid, by Merck Group (1 mentions)
Calcium chloride, by Merck Group (1 mentions)
Formic acid, by Merck Group (1 mentions)
13 protocols using sodium phosphate
1
NMR Metabolomics Sample Preparation
2
Culturing Yarrowia lipolytica Strain
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Yeast strain Yarrowia lipolytica CLIB183 was obtained from the International Center for Microbial Resources (CIRM), Grignon, France.
Glucose, glycerol, trehalose and propidium iodide were furnished by Sigma-Aldrich. Sodium chloride, sodium phosphate and aluminum plates were purchased from VWR. Yeast extract, bactopeptones, agar type E, citric acid, Chemchrome V8 (containing carboxyfluorescein di-acetate) and acetone were provided by Organotechnie, Becton Dickinson and Company, Biokar, Acros Organics, AES-Chemunex and Fisher Chemical, respectively.
Glucose, glycerol, trehalose and propidium iodide were furnished by Sigma-Aldrich. Sodium chloride, sodium phosphate and aluminum plates were purchased from VWR. Yeast extract, bactopeptones, agar type E, citric acid, Chemchrome V8 (containing carboxyfluorescein di-acetate) and acetone were provided by Organotechnie, Becton Dickinson and Company, Biokar, Acros Organics, AES-Chemunex and Fisher Chemical, respectively.
3
Quantitative Serum Protein Assay
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Sodium chloride (Cat. 0241-500G), sodium phosphate (Cat. 0404-500G), methanol (Cat. 85800.320) and acetonitrile (Cat. 83642.320) were purchased from VWR Life Science (Radnor, PA, USA). Ammonium bicarbonate (Cat. 11213–1 KG) was purchased from Honeywell (Charlotte, NA, USA). Acetic acid (Cat. 1.000.63.100), formic acid (Cat. 1.00264.1000), calcium chloride (Cat. 2382) and hydrochloric acid (Cat. 1.00317.1000) were purchased from Merck (Darmstadt, Germany). Dried synthetic peptides were purchased from Vivitide (Gardner, MA, USA) with greater than 95 % purity, isotope-labelled lysine (13C6,15 N2) and arginine (13C6,15 N4) residues. Peptide stock solutions were made by resuspension in ultrapure water to a concentration of 10 µg/mL and stored at −20 °C in glass vials. Pierce mass spectrometry-grade trypsin protease (Cat. 90058) was purchased from Thermo Fisher Scientific (Waltham, MA, USA). Trypsin stock solutions were made by resuspension in Acetic acid (50 mM) to a concentration of 0.1 g/L and stored at 4 °C. Purified horse IgG (AS10867) was purchased from Agrisera (Vännäs, Sweden). For spiking of serum samples we used minute surplus ocrelizumab (Ocrevus, Roche, Basel, Switzerland) volumes available after infusion of patients included in the OVERLORD-MS study (see below). Ultrapure water was obtained from a Milli-Q Advantage A10 (Merck Millipore, Burlington, MA, USA).
4
Kinetic Analysis of Thrombin-Catalyzed Reactions
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Kinetic data of thrombin-catalyzed reactions were obtained by diluting a stock solution of human α-thrombin (Cayman Chemicals, #13188; MW 38 kDa) to a final concentration of 1.2 nM in TEMg 1× buffer. Depending on the sample, DNA origamis or free TBAs were added with a final concentration of 1 nM. ssDNA samples were added with 2 nM to compensate for the lack of scaffold. Phosphate samples contained 14.5 μM sodium phosphate (VWR Chemicals) to simulate the negative charges. Fifty microliters of the mixture was pipetted to each well of a 96-well plate (Corning, #3991) and incubated at 37°C for 1 hour. Last, 50 μl of the respective substrate solution (0 to 25 μM final concentration, diluted in TEMg 1× buffer; Intavis) was added to each well. Plates were transferred to a multiplate reader (TECAN Spark10M), and fluorescence was measured immediately every minute for 80 min in total. Each sample was measured as a triplicate. The kinetic parameters of the enzymatic reaction were extracted using the OriginPro software.
5
Purification and Conjugation of Biomolecules
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Unless stated otherwise,
all chemicals and solvents were obtained from Sigma-Aldrich or Fisher
Scientific and used as received. Dimethyl sulfoxide (DMSO) (molecular
biology grade) and mushroom tyrosinase enzyme was obtained from Sigma-Aldrich.
Dithiothreitol (DTT) was obtained from Fisher Scientific. 2-Mercaptoethanol
(BME) and tetramethylenediamine were obtained from Acros. Sodium dodecyl
sulfate (SDS), bromophenol blue, coomassie brilliant blue G250, sodium
phosphate, sodium chloride, and Tris were obtained from VWR. Precision
Plus Protein Dual Color Standards was obtained from Bio-Rad. Glycine
was obtained from Applichem. BCN-POE3-NH–lissamine rhodamine
B conjugate was obtained from SynAffix. MeTz-TAMRA was obtained from
BroadPharm. Trifluoroacetic acid (TFA), formic acid (FA), and MeCN
were obtained from BioSolve.
Trastuzumab antibodies were ordered
from Evitria AG and purified by Hitrap protein A HP (ProtA) column
(5 mL) on an Agilent 1260 Preparative HPLC with diode-array detector
(Section 4.6 ). The
antibody, present in sterile filtered CHO media samples, were applied
to the ProtA column without any pretreatment.
Sortase A,42 (link) UCHT1 variants,15 (link) and IL-2 variants14 (link),43 (link) were obtained
and purified as reported.
all chemicals and solvents were obtained from Sigma-Aldrich or Fisher
Scientific and used as received. Dimethyl sulfoxide (DMSO) (molecular
biology grade) and mushroom tyrosinase enzyme was obtained from Sigma-Aldrich.
Dithiothreitol (DTT) was obtained from Fisher Scientific. 2-Mercaptoethanol
(BME) and tetramethylenediamine were obtained from Acros. Sodium dodecyl
sulfate (SDS), bromophenol blue, coomassie brilliant blue G250, sodium
phosphate, sodium chloride, and Tris were obtained from VWR. Precision
Plus Protein Dual Color Standards was obtained from Bio-Rad. Glycine
was obtained from Applichem. BCN-POE3-NH–lissamine rhodamine
B conjugate was obtained from SynAffix. MeTz-TAMRA was obtained from
BroadPharm. Trifluoroacetic acid (TFA), formic acid (FA), and MeCN
were obtained from BioSolve.
Trastuzumab antibodies were ordered
from Evitria AG and purified by Hitrap protein A HP (ProtA) column
(5 mL) on an Agilent 1260 Preparative HPLC with diode-array detector
(
antibody, present in sterile filtered CHO media samples, were applied
to the ProtA column without any pretreatment.
Sortase A,42 (link) UCHT1 variants,15 (link) and IL-2 variants14 (link),43 (link) were obtained
and purified as reported.
6
NMR Spectroscopy Sample Preparation
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Methanol, sodium phosphate, dibasic (Na2HPO4), sodium hydroxide, hydrochloric acid (32% m/v), and sodium azide (NaN3) were obtained from VWR International (Darmstadt, Germany). 3-(Trimethylsilyl)propionic-2,2,3,3-d4 acid sodium salt (TSP) was obtained from Alfa Aesar (Karlsruhe, Germany). Deuterium oxide (D2O) was obtained from Cambridge Isotope Laboratories, Inc. (Tewksbury, MA). Deionized water was purified using an in-house Milli-Q® Advantage water purification system from Millipore (Schwalbach, Germany).
7
Preparation of Phosphate NMR Buffer
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Sodium phosphate, dibasic (Na2HPO4), sodium hydroxide (NaOH), hydrochloric acid (HCl, 32% m/v), and sodium azide (NaN3) were obtained from VWR International (Darmstadt, Germany). 3(Trimethylsilyl) propionic acid-2,2,3,3-d4 sodium salt (TSP) was obtained from Alfa Aesar (Karlsruhe, Germany). Deuterium oxide (D2O) was obtained from Cambridge Isotopes Laboratories (Tewksbury, MA, USA). Deionized water was purified using an in-house Milli-Q Advantage Water Purification System from Millipore (Schwalbach, Germany). All chemicals were used without further purification. The phosphate NMR buffer solution was prepared by dissolving 5.56 g of anhydrous Na2HPO4, 0.4 g of TSP, and 0.2 g NaN3, in 400 mL of D2O and adjusted to pH 7.4 with 1 M NaOH and HCl. Upon addition of D2O to a final volume of 500 mL the pH was re-adjusted to pH 7.4 with 1 M NaOH and HCl.
8
Preparation of NMR Phosphate Buffer
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Sodium phosphate, dibasic (Na 2 HPO 4 ), sodium hydroxide, hydrochloric acid (32% m/v), and sodium azide (NaN 3 ) were obtained from VWR International (Darmstadt, Germany). 3(trimethylsilyl) propionic acid-2,2,3,3-d 4 sodium salt (TSP) was obtained from Alfa Aesar (Karlsruhe, Germany). Deuterium oxide (D 2 O) was obtained from Cambridge Isotopes laboratories (Tewksbury, MA). Deionized water was purified using in-house Milli-Q Advantage Water Purification System from Millipore (Schwalbach, Germany). The phosphate NMR buffer solution was prepared by dissolving 5.56 g of anhydrous Na 2 HPO4, 0.4 g of TSP, and 0.2 g NaN 3 , in 400 mL of D 2 O and adjusted to pH 7.4 with 1 M NaOH and HCl. Upon addition of D 2 O to a final volume of 500 mL, the pH was readjusted to pH 7.4 with 1 M NaOH and HCl.
9
Quantitative Metabolite Profiling
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L-arginine was obtained from AppliChem (Germany), ω-N G , N G -asymmetric dimethylarginine (ADMA), ω-N G -N' G -symmetric dimethylarginine (SDMA), and ω-N G -monomethylarginine (MMA) were obtained from Santa Cruz Biotechnology, Inc. (Germany). Sodium phosphate, dibasic (Na2HPO4), sodium hydroxide, chloroform, hydrochloric acid (32% m/v), and sodium azide (NaN3) were obtained from VWR International, 3-(trimethylsilyl) propionic acid-2,2,3,3-d4 sodium salt (TSP) from Alfa Aesar (Karlsruhe, Germany), deuterium oxide ( 2 H2O) from Cambridge Isotope laboratories, Inc. (Tewksbury, MA).
Adenosine, periodate oxidized (AdOx) and MS023 hydrochloride were obtained from Sigma Aldrich Austria. GSK591 (Synonyms: EPZ015866; GSK3203591 and GSK3368715 dihydrochloride (Synonyms: EPZ019997 dihydrochloride) were obtained from MedChemExpress Austria.
Adenosine, periodate oxidized (AdOx) and MS023 hydrochloride were obtained from Sigma Aldrich Austria. GSK591 (Synonyms: EPZ015866; GSK3203591 and GSK3368715 dihydrochloride (Synonyms: EPZ019997 dihydrochloride) were obtained from MedChemExpress Austria.
10
Collagen-based Wound Dressing Formulation
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Collagen (col) type I was obtained in our laboratory from fish scales of Esox lucius [38 (link)]. Gelatin type A (gel), hydroxyethyl cellulose (hec), Span 85, 1-ethyl-3(3-dimethylaminopropyl) carbodiimide (EDC), N-hydroxysuccinimide (NHS), Folin-Ciocalteu reagent, and gallic acid were purchased from Sigma-Aldrich (Poznan, Poland). Hydrochloric acid, acetic acid, sodium biphosphate anhydrous, sodium phosphate, sodium acetate anhydrous, and sodium chloride were obtained from Avantor Performance Materials Poland S.A. (Gliwice, Poland). Ethyl alcohol, paraffin oil, disodium edetate dihydrate, and sodium carbonate were supplied by the Stanlab (Lublin, Poland). The hydroglycolic Calendula officinalis flower extract was acquired from Provital S.A. (Barcelona, Spain).
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