Total RNA from RAW 264.7 cells or aortas was isolated using an RNeasy minikit (74104; Qiagen, Venlo, the Netherlands). RNA (1 μg) was used to synthesize cDNA by reverse transcription with an RT2 first-strand kit (330401; Qiagen). Real-time PCR analysis was performed on an ABI 7300 system (Thermo Fisher Scientific) with RT2 first SYBR green/ROX PCR master mix (330530; Qiagen). Specific primers for each gene were purchased from Qiagen. Genes of interest were normalized to mouse β-actin.
Rt2first sybr green rox pcr master mix
Manufactured by Qiagen
Sourced in Germany
The RT2 first SYBR green/ROX PCR master mix is a ready-to-use solution for real-time PCR amplification of DNA sequences. It contains SYBR Green I dye for detection and ROX passive reference dye for normalization.
Automatically generated - may contain errors
3 protocols using rt2first sybr green rox pcr master mix
1
RNA Isolation and qPCR Analysis of RAW 264.7 and Aorta Samples
2
B16-F10 Cell Total RNA Isolation and Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Total RNA from B16-F10 cells was isolated using an RNeasy minikit
(74104; Qiagen, Hilden, Germany). By reverse transcription with an
RT2 first-strand kit (330401; Qiagen, Hilden, Germany), RNA (1
μg) was used to synthesize cDNA. Real-time PCR analysis was performed on
an ABI 7300 system (Thermo Fisher Scientific, Waltham, MA) with RT2first SYBR green/ROX PCR master mix (330530; Qiagen, Hilden, Germany). Specific
primers for each gene were purchased from Qiagen. Genes of interest were
normalized to mouse β-actin.
(74104; Qiagen, Hilden, Germany). By reverse transcription with an
RT2 first-strand kit (330401; Qiagen, Hilden, Germany), RNA (1
μg) was used to synthesize cDNA. Real-time PCR analysis was performed on
an ABI 7300 system (Thermo Fisher Scientific, Waltham, MA) with RT2first SYBR green/ROX PCR master mix (330530; Qiagen, Hilden, Germany). Specific
primers for each gene were purchased from Qiagen. Genes of interest were
normalized to mouse β-actin.
3
B16-F10 Cell Total RNA Isolation and Analysis
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