Hrp conjugated goat anti mouse or anti rabbit secondary antibodies for western blot

Rabbit antibodies directed against TBK1 (D1B4), IRF3 (D83B9) and pIRF3 (4D4G) were purchased from Cell Signaling Technology. Rabbit anti-VISA (also known as MAVS, polyclonal antibody raised against residues 1–13 of MAVS), mouse anti-mitochondria (MTC02, recognizes a 60 kD non-glycosylated protein component of mitochondria) and mouse anti-c-Myc antibodies were obtained from Abcam. Rabbit polyclonal antibody to Flag-tag and mouse monoclonal antibody to β-actin were purchased from Sigma. Antibodies against Histone3 and α-tubulin have been previously described61 (link). The antisera of nsp4 and GP5 were prepared by our lab. HRP-conjugated goat anti-mouse or anti-rabbit secondary antibodies for Western blot were purchased from Santa Cruz. FITC-conjugated goat anti-rabbit and TRITC-conjugated goat anti-mouse secondary antibodies for confocal microscopy were purchased from Jackson ImmunoResearch. Poly(I:C) was purchased from InvivoGen.

Anti-TBK1/NAK (3504), anti-phospho-TBK1/NAK (5483), anti-IRF3 (10949), anti-phospho-IRF3 (4947), anti-NF-κB-p65 (8242), anti-phospho-NF-κB-p65 (3033), anti-TRAF6 (8028), anti-ub (20326), anti-ub-k63 (5621), anti-Myc-Tag (2276) and anti-Flag-Tag (14793) were purchased from Cell Signaling Technology (USA). Anti-phospho-IRF-3(Ser396) (SAB5700435), anti-HA-tag (H6908), anti-β-actin (A5441), and anti-Flag M2 Affinity Gel (A2220) were purchased from Sigma (USA). Anti-ub-k48 (EP8589) was purchased from Abcam (UK). Protein G Sepharose 4 Fast Flow was from GE Healthcare (Sweden). HRP-conjugated goat anti-mouse or anti-rabbit secondary antibodies for Western blot were purchased from Santa Cruz (USA). JetPRIME kit was obtained from Polyplus Transfection (France). Anti-pA151R polyclonal antibody was produced in rabbit by immunization with recombinant pA151R. Double-Luciferase Reporter Assay Kit was from Promaga (USA). 2’3’-cGAMP, poly(dA:dT), and Lipofectamine 3000 were purchased from Invitrogen (USA). 3-Methyladenine (3-MA, HY-19312), MG-132 (HY-13259), E-64 (HY-15282), and Z-VAD-FMK (HY-16658B) were from MedChemExpress (MCE) (USA).