Orca c4742 95 12er camera

Manufactured by Hamamatsu Photonics

Sourced in Japan

The Orca C4742-95-12ER is a high-performance digital camera designed for scientific and industrial applications. It features a 1.3-megapixel CCD sensor with a pixel size of 6.45 x 6.45 μm. The camera is capable of capturing images at a maximum resolution of 1280 x 1024 pixels. It supports a frame rate of up to 12 frames per second and provides 12-bit depth image data.

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Lab products found in correlation

Eclipse te2000 u inverted fluorescence microscope, by Nikon (1 mentions) Openlab 5, by PerkinElmer (1 mentions) Softworx version 4, by Cytiva (1 mentions)

Close spelling variants of this product

C4742-95 (83 citations) C4742-95 camera (16 citations) C4742 (12 citations) C4742-95-12ER (6 citations) Orca C4742-95-12ER charge-coupled device camera (6 citations) Orca C4742-95 (3 citations) Orca C4742-95-12ER (2 citations)

2 protocols using orca c4742 95 12er camera

Msn2-GFP Localization Assay

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For Msn2-GFP localisation studies, exponentially growing cells transformed with the pADH1-MSN2-GFP plasmid treated with CAS (15 ng/ml) or CFW (5 µg/ml) for two hours were directly visualized and analysed by fluorescence microscopy and differential interference contrast (DIC) using a Nikon Eclipse TE2000-U fluorescence inverted microscope equipped with CCD. Digital images were acquired with an Orca C4742-95-12ER camera (Hamamatsu Photonics, Japan) and processed with the Hamamatsu HCImage imaging system software. Nuclei were stained by addition of 10 µg/ml of DAPI to the cultures 10 min before microscopy.

García R., Bravo E., Diez-Muñiz S., Nombela C., Rodríguez-Peña J.M, & Arroyo J. (2017). A novel connection between the Cell Wall Integrity and the PKA pathways regulates cell wall stress response in yeast. Scientific Reports, 7, 5703.

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Spore-Autonomous Fluorescence Recombination Analysis

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The spore-autonomous fluorescence recombination analysis was performed as described previously (Thacker et al., 2011 (link)). After synchronization, diploid yeast cells were inoculated into SPM to OD600 ~3.5 and incubated at 30°C. After 48–60 hrs, images were captured in four channels using a DeltaVision personalDV multiplexed with a 60× 1.4NA DIC Oil PlanApoN objective and Roper CoolSnap HQ2 camera under the control of Softworx Version 4.1.0 (Applied Precision) or a Leica DM 6000B microscope using a HCX PL Fluotar 63×, oil objective lens, and captured using an ORCA C4742-95-12ER camera (Hamamatsu) controlled by Openlab 5.0.2 software (Improvision). The pattern of fluorescence in the tetrads was manually scored using Fiji. Only tetrads with four spores and each fluorescence marker occurring in two spores were included in the final analysis. Recombination frequency, expressed as map distance in centimorgans with standard error, was calculated using the Stahl lab online tools. Crossover interference ratio was calculated as described (Thacker et al., 2011 (link)). For every strain, > 430 tetrads were scored based on at least two independent experiments.

Arter M., Hurtado-Nieves V., Oke A., Zhuge T., Wettstein R., Fung J.C., Blanco M.G, & Matos J. (2018). Regulated crossing-over requires inactivation of Yen1/GEN1 resolvase during meiotic prophase I. Developmental cell, 45(6), 785-800.e6.

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