Recombinant sars cov 2 s1 protein

Manufactured by Creative Diagnostics

Recombinant SARS-CoV-2-S1 protein is a laboratory product that contains the S1 subunit of the spike protein of the SARS-CoV-2 virus. The S1 subunit is a key component of the virus's envelope and plays a crucial role in the virus's ability to bind to and enter human cells. This recombinant protein is produced using genetic engineering techniques and can be used in various research and diagnostic applications.

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Lab products found in correlation

Cd27 apc, by BioLegend (2 mentions) Cd19 bv786, by BD (2 mentions) Cruzfluor647, by Santa Cruz Biotechnology (2 mentions) Cd38 fitc, by BD (2 mentions) Cd19 pe, by BioLegend (2 mentions) 7 aad, by Thermo Fisher Scientific (2 mentions) Cd38 pe cy7, by BioLegend (2 mentions) Cd27 pe, by BD (2 mentions)

2 protocols using recombinant sars cov 2 s1 protein

SARS-CoV-2 Antibody Profiling with Flow Cytometry

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Recombinant SARS-CoV-2-S1 protein produced in HEK cells (Creative Diagnostics, DAGC091) was covalently labeled using CruzFluor647 (Santa Cruz Biotechnology, sc-362620) according to the manufacturer’s instructions.
Using fluorescence-activated cell sorting we sorted viable single cells from freshly isolated peripheral blood mononuclear cells (PBMCs as 7AAD⁻CD19⁺CD27⁺CD38⁺ antibody-secreting cells (ASCs) or SARS-CoV2-S1-enriched 7AAD⁻CD19⁺CD27⁺ memory B cells (MBCs) into 96-well PCR plates. Staining was performed on ice for 25 minutes in PBS with 2 % FCS using the following antibodies: 7-AAD 1:400 (Thermo Fisher Scientific), CD19-BV786 1:20 (clone SJ25C1, BD Biosciences, 563326), CD27-PE 1:5 (clone M-T271, BD Biosciences, 555441), CD38-FITC 1:5 (clone HIT2, BD Biosciences, 560982), and SARS-CoV-S1-CF647 at 1 μg/ml for patients CV07, CV38, CV23, CV24, CV 38, CV48, CV-X1, CV-X2 and CV01 (second time point, fig. S1). The first patients (CV01 (first time point), CV03, and CV05) were stained with a divergent set of antibodies: CD19-PE 1:50 (clone HIB19, BioLegend, 302207), CD38-PEcy7 1:50 (clone HIT2, BioLegend, 303505), CD27-APC 1:50 (clone O323, BioLegend, 302809) and DAPI as viability dye.

Kreye J., Reincke S.M., Kornau H.C., Sánchez-Sendin E., Max Corman V., Liu H., Yuan M., Wu N.C., Zhu X., Lee C.C., Trimpert J., Höltje M., Dietert K., Stöffler L., von Wardenburg N., van Hoof S., Homeyer M.A., Hoffmann J., Abdelgawad A., Gruber A.D., Bertzbach L.D., Vladimirova D., Li L.Y., Barthel P.C., Skriner K., Hocke A.C., Hippenstiel S., Witzenrath M., Suttorp N., Kurth F., Franke C., Endres M., Schmitz D., Jeworowski L.M., Richter A., Schmidt M.L., Schwarz T., Müller M.A., Drosten C., Wendisch D., Sander L.E., Osterrieder N., Wilson I.A, & Prüss H. (2020). A SARS-CoV-2 neutralizing antibody protects from lung pathology in a COVID-19 hamster model. bioRxiv.

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Isolation and Identification of SARS-CoV-2 Antibody-Secreting Cells

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Recombinant SARS-CoV-2-S1 protein produced in HEK cells (Creative Diagnostics, DAGC091) was covalently labeled using CruzFluor647 (Santa Cruz Biotechnology, sc-362620) according to the manufacturer’s instructions.
Using fluorescence-activated cell sorting we sorted viable single cells from freshly isolated peripheral blood mononuclear cells (PBMCs as 7AAD^-CD19⁺CD27⁺CD38⁺ antibody-secreting cells (ASCs) or SARS-CoV2-S1-enriched 7AAD^-CD19⁺CD27⁺ memory B cells (MBCs) into 96-well PCR plates. Staining was performed on ice for 25 minutes in PBS with 2% FCS using the following antibodies: 7-AAD 1:400 (Thermo Fisher Scientific), CD19-BV786 1:20 (clone SJ25C1, BD Biosciences, 563326), CD27-PE 1:5 (clone M-T271, BD Biosciences, 555441), CD38-FITC 1:5 (clone HIT2, BD Biosciences, 560982), and SARS-CoV-S1-CF647 at 1 μg/ml for patients CV07, CV38, CV23, CV24, CV 38, CV48, CV-X1, CV-X2 and CV01 (second time point, fig. S1). The first patients (CV01 (first time point), CV03, and CV05) were stained with a divergent set of antibodies: CD19-PE 1:50 (clone HIB19, BioLegend, 302207), CD38-PEcy7 1:50 (clone HIT2, BioLegend, 303505), CD27-APC 1:50 (clone O323, BioLegend, 302809) and DAPI as viability dye.

Kreye J., Reincke S.M., Kornau H.C., Sánchez-Sendin E., Corman V.M., Liu H., Yuan M., Wu N.C., Zhu X., Lee C.C., Trimpert J., Höltje M., Dietert K., Stöffler L., von Wardenburg N., van Hoof S., Homeyer M.A., Hoffmann J., Abdelgawad A., Gruber A.D., Bertzbach L.D., Vladimirova D., Li L.Y., Barthel P.C., Skriner K., Hocke A.C., Hippenstiel S., Witzenrath M., Suttorp N., Kurth F., Franke C., Endres M., Schmitz D., Jeworowski L.M., Richter A., Schmidt M.L., Schwarz T., Müller M.A., Drosten C., Wendisch D., Sander L.E., Osterrieder N., Wilson I.A, & Prüss H. (2020). A Therapeutic Non-self-reactive SARS-CoV-2 Antibody Protects from Lung Pathology in a COVID-19 Hamster Model. Cell, 183(4), 1058-1069.e19.

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