Anti mouse igg h l alexa fluor 594

S2R+ cells were plated in 24-well plates in serum-free media on poly-l-lysine coated coverslips, and were treated with 1 ug dsRNA for 50 minutes, after which complete medium was added. Four days later, cells were stressed by replacing the media with fresh media containing 0.2 mM sodium arsensite (Sigma-Aldrich, St. Louis, MO). Two hours after the initiation of the sodium arsenite treatment, cells were fixed in 4% paraformaldehyde in phosphate-buffered saline (PBS). The rest of the immunofluorescence protocol was identical to that with the HEK cells. Primary antibodies used: anti-dFMR1 (5A11, 1:200, DSHB). Secondary antibodies used: anti-Mouse IgG (H+L) Alexa Fluor 594 (#A-11005, 1:1000, Invitrogen, Carlsbad, CA). Images were acquired with Leica DMI6000 inverted fluorescent microscope with an oil immersion X100 objective and 1.6 magnification factor. Quantification of stress granules was performed with CellProfiler.