Histone h3 d1h2 xp rabbit mab

TOPK and β-actin were purchased from Santa Cruz Technology, Inc (Santa Cruz, CA, USA). Phospho-Src (Tyr416) (D49G4) Rabbit mAb, Src (32G6) Rabbit mAb, Histone H3 (D1H2) XP Rabbit mAb, phospho-Histone H3 (Ser10) (D2C8) XP Rabbit mAb, and HA-Tag Rabbit polyclonal antibody were purchased from Cell Signaling Technology, Inc (Boston, MA, USA). Ubiquitin Rabbit polyclonal antibody was purchased from Proteintech Group, Inc (Chicago, USA). His-Tag Mouse monoclonal antibody (4E6) and HA-Tag Mouse monoclonal antibody (1B10) were obtained from Pregene, Inc (Beijing, China). HRP-labeled Goat anti Mouse IgG (H+L) and Goat anti Rabbit IgG (H+L) were purchased from EarthOx, LLC (San Francisco, CA, USA). Donkey anti-Rabbit IgG (H+L) Alexa Fluor^® 546 Red and Donkey anti-Mouse IgG (H+L) Alexa Fluor^® 488 Green were purchased from Invitrogen, Inc (Carlsbad, CA, USA). Phospho-TOPK at Y74 or Y272 antibodies were prepared by Abgent, Inc (Suzhou, China). All antibodies were used following the instructions of the respective manufacturers. The small hairpin RNA constructs against Src (5′-GACAGACCTGTCCTTCAAGAA-3′) used in this study was from the BioMedical Genomics Center at the University of Minnesota. Dasatinib was purchased from Selleckchem, Inc (Houston, TX, USA).